Investigation of biomarkers that enable early diagnosis of graft-versus-host disease

• Project/Area Number: 24790960
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Hematology
• Research Institution: University of Tsukuba
• Principal Investigator: KURITA Naoki 筑波大学, 医学医療系, 講師 (30555561)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 移植片対宿主病 / Notchシグナル / 移植片対腫瘍効果 / 同種造血幹細胞移植 / Notch / NK細胞 / 臍帯血移植 / 再発 / GVHD

Research Abstract

Graft-versus-host disease (GVHD) is a fatal complication of allogeneic stem-cell transplantation (allo-SCT). GVHD-model mice showed that Notch signaling of lymphocytes exacerbates GVHD. Therefore we conducted comprehensive analysis of Notch expression on lymphocytes after allo-SCT by using human samples. As a result, Notch1 was expressed only on NK cells and the expression was significantly higher in recipients of cord-blood transplantation. Not GVHD exacerbation, but reduction of relapse was seen in the recipients whose NK cells expressed Notch1 strongly. This result showed the possibility that enhancing graft-versus-tumor effect without worsening GVHD by manipulating Notch signal.

Research Products

• [Presentation] 同種造血幹細胞移植後の末梢血リンパ球における, Notch 分子の発現解析 (2014)
  • Author(s): 栗田尚樹
  • Organizer: 第36回日本造血幹細胞移植学会総会
  • Place of Presentation: 宜野湾
• [Presentation] 同種造血幹細胞移植後の末梢血リンパ球における，Notch分子の発現解析 (2014)
  • Author(s): 栗田尚樹、桃林篤、関正則、横山泰久、坂田（柳元）麻実子、小原直、長谷川雄一、千葉滋
  • Organizer: 第36回日本造血細胞移植学会総会
  • Place of Presentation: 沖縄コンベンションセンター
• [Presentation] Comprehensive analysis of Notch expression on lymphocytes after allogeneic stem cell transplantation (2013)
  • Author(s): 栗田尚樹
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌
• [Presentation] Comprehensive analysis of Notch expression on lymphocytes after allogeneic stem cell transplantation (2013)
  • Author(s): Naoki Kurita, Atsushi Momobayashi, Masanori Seki, Yasuhisa Yokoyama, Mamiko Sakata-Yanagimoto, Naoshi Obara, Yasushi Okoshi, Kazumi Suzukawa, Yuichi Hasegawa, Hideo Yagita, Shigeru Chiba
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: さっぽろ芸文館