• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The role of megakaryocytic signals in Evi1 leukemia

Research Project

Project/Area Number 24790961
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Hematology
Research InstitutionThe University of Tokyo

Principal Investigator

OKOCHI Naoko  東京大学, 医学部附属病院, 助教 (00568412)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsEvi1 / leukemia / model mouse / CD41 / cMpl / TPO
Research Abstract

Ecotropic viral integration site 1 (Evi1), located on chromosome 3q26, is an independent negative prognostic indicator of survival in acute myeloid leukemia (AML). Thrombocytosis is a characteristic feature in AML patients with 3q26 abnormality, and the platelet function related genes, such as CD41 (integrin aIIb) and cMpl, positively correlates with Evi1 expression in AML patients. Previously, we succeeded in making a mouse model of Evi1-related leukemia. Sub-population of this Evi1 leukemia mice cells expressed both CD41 and cMpl. The CD41+ fraction of Evi1 leukemia mice more efficiently induced secondary leukemia than the CD41- counterpart in a serial BMT assay. The CD41+ cells predominantly expressed cMpl, and shRNA-mediated knockdown of cMpl delayed the progression of Evi1 leukemia in vivo, suggesting that THPO/cMpl signaling is involved in enhancing the growth and survival of Evi1 leukemia cells.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (18 results)

All 2014 2013 2012 Other

All Journal Article (12 results) (of which Peer Reviewed: 2 results) Presentation (4 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Single-cell gene expression analysis reveals clonal architecture of blast-phase chronic myeloid leukaemia2014

    • Author(s)
      Nukina A, Kagoya Y, Watanabe-Okochi N, Arai S, Ueda K, Yoshimi A, Nannya Y, Kurokawa M
    • Journal Title

      Br J Haematol

      Volume: (Epub ahead of print)

    • Related Report
      2013 Final Research Report
  • [Journal Article] The role of alloantibodies against human platelet antigen-15 in multiply platelet transfused patients2014

    • Author(s)
      Matsuhashi M, Tsuno NH, Sone S, Mishima Y, Nagura Y, Watanabe-Okochi N, Ikeda T, Kashiwase K, Fukuda S, Iriyama T, Hyodo H, Yamashita T, Kamei Y, Arai S, Minami M, Fujii T, Kurokawa M, Tozuka M, Takahashi K, Santoso S
    • Journal Title

      Transfusion

      Volume: 54(4) Pages: 1093-9

    • Related Report
      2013 Final Research Report
  • [Journal Article] Safety and efficacy of preoperative autologous blood donation for high-risk pregnant women : experience of a large university hospital in Japan2014

    • Author(s)
      Yamamoto Y, Yamashita T, Tsuno NH, Nagamatsu T, Okochi N, Hyodo H, Ikeda T, Kawabata M, Kamei Y, Nagura Y, Sone S, Fujii T, Takahashi K, Kozuma S
    • Journal Title

      J Obstet Gynaecol Res

      Volume: 40 Pages: 1308-16

    • Related Report
      2013 Final Research Report
  • [Journal Article] The shortest isoform of C/EBPβ, Liver inhibitory protein (LIP), collaborates with Evi1 to induce AML in a mouse BMT model2013

    • Author(s)
      Watanabe-Okochi N, Yoshimi A, Sato T, Ikeda T, Kumano K, Taoka K, Satoh Y, Shinohara A, Tsuruta T, Masuda A, Yokota H, Yatomi Y, Takahashi K, Kitaura J, Kitamura T, Kurokawa M
    • Journal Title

      Blood

      Volume: 121(20) Pages: 4142-55

    • NAID

      10031196292

    • Related Report
      2013 Final Research Report
  • [Journal Article] Single-cell gene expression analysis reveals clonal architecture of blast-phase chronic myeloid leukaemia.2013

    • Author(s)
      Nukina A, Kagoya Y, Watanabe-Okochi N, Arai S, Ueda K, Yoshimi A, Nannya Y, and Kurokawa M.
    • Journal Title

      Br J Haematol

      Volume: In Press Issue: 3 Pages: 580-91

    • DOI

      10.1111/bjh.12726

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prediction model for CD34 positive cell yield in peripheral blood stem cell collection on the fourth day after G-CSF administration in healthy donors2013

    • Author(s)
      Yoshizato T, Watanabe-Okochi N, Nannya Y, Ichikawa M, Takahashi T, Sato T, Masuda A, Yatomi Y, Tsuno NH, Kurokawa M, Takahashi K
    • Journal Title

      Int J Hematol

      Volume: 98(1) Pages: 56-65

    • Related Report
      2013 Final Research Report
  • [Journal Article] A role for RUNX1 in hematopoiesis and myeloid leukemia2013

    • Author(s)
      Ichikawa M, Yoshimi A, Nakagawa M, Nishimoto N, Watanabe-Okochi N, Kurokawa M
    • Journal Title

      Int J Hematol

      Volume: 97(Review) Pages: 726-34

    • Related Report
      2013 Final Research Report
  • [Journal Article] The current status of autologous blood transfusion in Japan - The importance of pre-deposit autologous blood donation program and the needs to achieve patient blood management2013

    • Author(s)
      Tsuno NH, Nagura Y, Kawabata M, Matsuhashi M, Sone S, Ikeda T, Okochi N, Takahashi K
    • Journal Title

      Transfus Apher Sci

      Volume: 49(3) Pages: 673-80

    • Related Report
      2013 Final Research Report
  • [Journal Article] The frequencies of SLC44A2 alleles among the Japanese population2013

    • Author(s)
      Matsuhashi M, Tsuno NH, Ikeda T, Mishima Y, Watanabe-Okochi N, Santoso S, Tozuka M, Takahashi K
    • Journal Title

      Tissue Antigens

      Volume: 81(4) Pages: 227-8

    • Related Report
      2013 Final Research Report
  • [Journal Article] The shortest isoform of C/EBPβ, Liver inhibitory protein (LIP), collaborates with Evi1 to induce AML in a mouse BMT model2013

    • Author(s)
      Watanabe-Okochi N, Yoshimi A, Sato T, Ikeda T, Kumano K, Taoka K, Satoh Y, Shinohara A, Tsuruta T, Masuda A, Yokota H, Yatomi Y, Takahashi K, Kitaura J, Kitamura T, Kurokawa M
    • Journal Title

      Rinsho Ketsueki

      Volume: 54(8) Pages: 749-58

    • NAID

      10031196292

    • Related Report
      2013 Final Research Report
  • [Journal Article] The shortest isoform of C/EBPβ, Liver inhibitory protein (LIP) , collaborates with Evi1 to induce AML in a mouse BMT model2013

    • Author(s)
      Okochi, N., Yoshimi, A., Sato, T., Ikeda, T., Kumano, K., Taoka, K., Sato, Y., Shinohara, A., Tsuruta, A., Masuda, A., Yokota, H., Yatomi, Y., Takahashi, K., Kitaura, J., Kitamura, T. and Kurokawa, M
    • Journal Title

      Blood

      Volume: 121 Issue: 20 Pages: 4142-4155

    • DOI

      10.1182/blood-2011-07-368654

    • NAID

      10031196292

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 輸血検査2013

    • Author(s)
      三島由布子、大河内直子
    • Journal Title

      臨床検査

      Volume: 1 Pages: 34-40

    • Related Report
      2012 Research-status Report
  • [Presentation] Evi1-induced leukemic cells express CD41 known as a megakaryocytic marker in a mouse BMT bone marrow transplantation model2012

    • Author(s)
      大河内直子
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Year and Date
      2012-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Evi1-induced leukemic cells express CD41 known as a megakaryocytic marker in a mouse BMT model2012

    • Author(s)
      大河内直子
    • Organizer
      第74回日本血液学会学術集会
    • Place of Presentation
      京都
    • Year and Date
      2012-10-19
    • Related Report
      2013 Final Research Report
  • [Presentation] Evi1-induced leukemic cells express CD41 known as a megakaryocytic marker in a mouse BMT model.2012

    • Author(s)
      大河内直子
    • Organizer
      第74回日本血液学会学術集会
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] Evi1-induced leukemic cells express CD41 known as a megakaryocytic marker in a mouse BMT bone marrow transplantation model.2012

    • Author(s)
      大河内直子
    • Organizer
      第35回日本分子生物学会年会
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
  • [Book] 輸血合併症 : 今日の治療と看護 改訂第3版2013

    • Author(s)
      大河内直子, 高橋孝喜
    • Related Report
      2013 Final Research Report
  • [Remarks] 東京大学医学部附属病院 血液・腫瘍内科 論文リスト

    • URL

      http://www.u-tokyo-hemat.com/research_ronbun.html

    • Related Report
      2012 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi