Project/Area Number |
24790968
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Hematology
|
Research Institution | Kanazawa University |
Principal Investigator |
|
Project Period (FY) |
2012-04-01 – 2014-03-31
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
|
Keywords | Loss of heterogeneity / Aplastic anemia / Human Leukocyte Antigen / 病態検査学 / 血液内科学 / 免疫学 / 再生不良性貧血 / uniparental disomy / HLA |
Research Abstract |
The percentage of 6pLOH was different among hematopoiesis stages in human bone marrow, and the progenitor cells showed much lower percentage of 6pLOH than that of MEP and GMP. I found approximately 30 times lower expression of CXCL12 which promotes the cell growth and proliferation cytokine than that of normal fraction with microarray analysis. As a result, I demonstrated the percentage of HLA deficient was significantly decreased, since the cell cycle was not promoted in the 6pLOH of CMP fraction with low CXCL12 and CXCR4 expression.
|