Optimization of chimeric antigen receptor-gene modified T lymphocyte generation
Project/Area Number |
24790969
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Hematology
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Research Institution | Nagoya University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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Keywords | キメラ抗原レセプター / 細胞療法 / 遺伝子導入T細胞 / 免疫療法 / リンパ性白血病 / 悪性リンパ腫 / 遺伝子導入T細胞療法 / CD8陽性Tリンパ球 / Tリンパ球 / CD8陽性 |
Outline of Final Research Achievements |
We aimed to optimize the method for generation of chimeric antigen receptor-gene modified T lymphocyte (CD19CAR-T). For the generation of allogeneic CD19CAR-T lymphocytes, we analyzed the method for better generation of virus-specific/ CD19CAR-gene modified T lymphocytes. We tested the possible combination of cytokine combination and concentration. As a result, the IL-2 and IL-21 combination was better than other conditions. For the optimization of autologous CD19CAR-T lymphocytes, we developed drug-inducible expression system of CD19CAR, and demonstrated that we could successfully control CD19CAR expression under drug administration.
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Report
(4 results)
Research Products
(35 results)
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[Journal Article] Target antigen density governs the efficacy of anti-CD20-CD28-CD3 ζ chimeric antigen receptor-modified effector CD8+ T cells.2015
Author(s)
Watanabe K, Terakura S, Martens AC, van Meerten T, Uchiyama S, Imai M, Sakemura R, Goto T, Hanajiri R, Imahashi N, Shimada K, Tomita A, Kiyoi H, Nishida T, Naoe T, Murata M.
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Journal Title
J Immunol.
Volume: 194(3)
Issue: 3
Pages: 911-20
DOI
Related Report
Peer Reviewed
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[Journal Article] Simple and Efficient Generation of Virus-specific T Cells for Adoptive Therapy Using Anti-4-1BB Antibody2015
Author(s)
Nobuhiko Imahashi, Tetsuya Nishida, Tatsunori Goto, Seitaro Terakura, Keisuke Watanabe, Ryo Hanajiri, Reona Sakemura, Misa Imai, Hitoshi Kiyoi, Tomoki Naoe, and Makoto Murata
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Journal Title
Journal of Immunotherapy
Volume: 38(2)
Issue: 2
Pages: 62-70
DOI
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Chimerism status after unrelated donor bone marrow transplantation with fludarabine-melphalan conditioning is affected by the melphalan dose and is predictive of relapse.2015
Author(s)
Imahashi N, Ohashi H, Terakura S, Miyao K, Sakemura R, Kato T, Sawa M, Yokohata E, Kurahashi S, Ozawa Y, Nishida T, Kiyoi H, Watamoto K, Kohno A, Kasai M, Kato C, Iida H, Naoe T, Miyamura K, Murata M
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Journal Title
Annals of Hematology
Volume: -
Related Report
Peer Reviewed
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[Journal Article] Dexamethasone palmitate ameliorates macrophages-rich graft-versus-host disease by inhibiting macrophage functions2014
Author(s)
Satoshi Nishiwaki, Takayuki Nakayama, Makoto Murata, Tetsuya Nishida, Seitaro Terakura, Shigeki Saito, Tomonori Kato, Hiroki Mizuno, Nobuhiko Imahashi, Aika Seto, Yukiyasu Ozawa, Koichi Miyamura, Masafumi Ito, Kyosuke Takeshita, Hidefumi Kato, Shinya Toyokuni, Keisuke Nagao, Ryuzo Ueda, and Tomoki Naoe
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Journal Title
PLOS one
Volume: 9(5)
Issue: 5
Pages: e96252-e96252
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Comparison of cord blood transplantation with unrelated bone marrow transplantation in patients older than fifty years.2014
Author(s)
Tanaka M, Miyamura K, Terakura S, Imai K, Uchida N, Ago H, Sakura T, Eto T, Ohashi K, Fukuda T, Taniguchi S, Mori S, Nagamura-Inoue T, Atsuta Y, Okamoto S.
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Journal Title
Biol Blood Marrow Transplant.
Volume: 21
Issue: 3
Pages: 517-525
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of a novel HLA-A^*24:02-restricted adenovirus serotype 11-specific CD8+ T-cell epitope for adoptive immunotherapy2013
Author(s)
Imahashi N, Nishida T, Ito Y, Kawada J, Nakazawa Y, Toji S, Suzuki S, Terakura S, Kato T, Murata M, Naoe T.
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Journal Title
Molecular Immunolgy
Volume: 56
Issue: 4
Pages: 399-405
DOI
Related Report
Peer Reviewed
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[Journal Article] Phase II study of dose-modified busulfan by real-time targeting in allogeneic hematopoietic stem cell transplantation for myeloid malignancy.2012
Author(s)
Kuwatsuka Y, Kohno A, Terakura S, Saito S, Shimada K, Yasuda T, Inamoto Y, Miyamura K, Sawa M, Murata M, et al.
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Journal Title
Cancer Science
Volume: 103
Issue: 9
Pages: 1688-1694
DOI
Related Report
Peer Reviewed
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[Journal Article] Escape of leukemia blasts from HLA-specific CTL pressure in a recipient of HLA one locus-mismatched bone marrow transplantation.2012
Author(s)
Kato T, Terakura S, Murata M, Sugimoto K, Murase M, Iriyama C, Tomita A, Abe A, Suzuki M, Nishida T, Naoe T.
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Journal Title
Cellular Immunology
Volume: 276
Issue: 1-2
Pages: 75-82
DOI
Related Report
Peer Reviewed
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[Presentation] Comparison of outcomes of unrelated BM and CB transplants in young adult leukemia patients. An Updated Results of Japanese Registration Data2014
Author(s)
Terakura S, Atsuta Y, Tsukada N, Kobayashi T, Tanaka M, Kanda J, Ohashi K, Fukuda T, Uchida N, Takahashi S, Nagamura-Inoue T, Morishima Y, Miyamura K
Organizer
第56回米国血液学会
Place of Presentation
サンフランシスコ、米国
Year and Date
2014-12-06 – 2014-12-09
Related Report
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[Presentation] Cord blood allograft rejection mediated by coordination of cellular and humoral immunity
Author(s)
Ryo Hanajiri, Makoto Murata, Kyoko Sugimoto, Miho Murase, Haruhiko Ohashi, Tatsunori Goto, Keisuke Watanabe, Nobuhiko Imahashi, Seitaro Terakura, Tetsuya Nishida, Tomoki Naoe
Organizer
第75回日本血液学会
Place of Presentation
札幌芸文館 (札幌市)
Related Report
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[Presentation] Identification of a novel HLA-A*2402-restricted adenovirus serotype 11-specific CD8+ T-cell epitope
Author(s)
Nobuhiko Imahashi, Tetsuya Nishida, Yoshinori Ito, Jun-Ichi Kawada, Yozo Nakazawa, Shingo Toji, Susumu Suzuki, Seitaro Terakura, Tomonori kato, Makoto Murata, Tomoki Naoe
Organizer
第75回日本血液学会
Place of Presentation
札幌市教育文化会館 (札幌市)
Related Report
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[Presentation] Anti-CD20 Chimeric Antigen Receptor Transduced T Cells Can Recognize Very Low Antigen Expression: Determination Of The Lower Threshold Required To Activate The CAR-T Cells
Author(s)
Keisuke Watanabe, Seitaro Terakura, Tatsunori Goto, Ryo Hanajiri, Nobuhiko Imahashi, Kazuyuki Shimada, Tetsuya Nishida, Akihiro Tomita, Makoto Murata, Tomoki Naoe
Organizer
第55回米国血液学会総会
Place of Presentation
New Orleans, USA
Related Report
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[Presentation] Cord Blood Allograft Rejection Mediated By Coordinated Donor-Specific Cellular and Humoral Immune Processes
Author(s)
Ryo Hanajiri, Makoto Murata, Kyoko Sugimoto, Miho Murase, Haruhiko Ohashi, Tatsunori Goto, Keisuke Watanabe, Nobuhiko Imahashi, Seitaro Terakura, Tetsuya Nishida, Tomoki Naoe
Organizer
第55回米国血液学会総会
Place of Presentation
New Orleans, USA
Related Report
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[Presentation] Impact of Macrophage Activation on Delayed Engraftment Following Allogeneic Hematopoietic Stem Cell Transplantation: Mac Ratio, a New Predictive Index
Author(s)
Naomi Kawashima, Satoshi Nishiwaki, Seitaro Terakura, Sonoko Kamoshita, Kyoko Watakabe, Emi Yokohata, Daisuke Koyama, Naoko Shimizu, Shingo Kurahashi, Yukiyasu Ozawa, Masafumi Ito, Koichi Miyamura
Organizer
第55回米国血液学会総会
Place of Presentation
New Orleans, USA
Related Report
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