| Project/Area Number |
24790972
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Hematology
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| Research Institution | Osaka University |
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Principal Investigator |
TOKUNAGA MASAHIRO 大阪大学, 医学(系)研究科(研究院), 助教 (90597410)
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| Research Collaborator |
HORIE Kyoji 奈良県立医科大学, 医学部, 教授 (30333446)
YAMANISHI Ayako 大阪大学, 医学系研究科, 特任研究員
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 血球分化 / ES細胞 / スクリーニング / ノックアウトマウス / 新規遺伝子 |
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| Research Abstract |
In this study, to screen for a mutant whose hematopoietic differentiation is disturbed, we co-cultured several homozygous mutant ES clones with OP9, a stromal cell line supporting hematopoietic differentiation. As a result, we found out that in one mutant clone, this differentiation was severely disturbed. The responsible gene of this mutant is a novel, uncharacterized gene (unpublished data). Then, we generated a knockout mouse of this gene. Intriguingly, null mutant mice were embryonic lethal by E6.5, demonstrating that this gene plays essential roles in early development. Further investigation of this gene may give a new insight in hematopoietic development.
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