Analysis of human HSCs-supporting mechanisms of human bone marrow-derived mesenchymal stromal cells and development of a Human HSC expansion system
Project/Area Number |
24790984
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Hematology
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Research Institution | Kansai Medical University |
Principal Investigator |
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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Keywords | 造血幹細胞 / 間葉系幹細胞 / 骨髄ニッチ / ニッチ |
Research Abstract |
I developed about 500 hybridoma clones which produce the monoclonal antibody (mAb) against human cord blood-derived 18Lin-CD34+/- fractions. In this fraction, human primitive hematopoietic stem cells (HSCs) are highly concentrated. Then, I performed screening of neutralization antibodies against the human HSC supportive activities of human bone marrow-derived mesenchymal stem cells (MSCs) using this antibody library. For this propose, these developed mAbs were added into coculture of MSC and 18Lin-CD34+/- cells. In the result, I found the antibody which inhibited (approximately 20%) the HSC supportive ability of MSCs from this antibody library. This screening is currently in progress.
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Report
(3 results)
Research Products
(16 results)
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[Presentation] Functional Significance of MPL Expression in the Human Primitive Hematopoietic Stem Cell Compartment2012
Author(s)
Takahashi Masaya, Matsuoka Yoshikazu, Iwaki Ryuji, Nakatsuka Ryusuke, Fujioka Tatsuya, Kohno Hirao, Matsui Kazuo, Asano Hiroaki, Sasaki Yutaka, Kaneko Kazunari, Sonoda Yoshiaki
Organizer
2012 ASH Annual Meeting
Place of Presentation
Georgia World Congress Center(アメリカ)
Related Report
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