| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
NUP98 is a component of the nuclear pore complex (NPC). The NUP98 gene is rearranged with several partner genes in acute myeloid leukemia and myelodysplastic syndromes and NUP98-related leukemia is known to have a poor prognosis. Although the NUP98 gene has the several fusion partners, the role of the NUP98 moiety of NUP98 fusion proteins is not well investigated. To investigate NUP98-fusion-interacting proteins,, we purified the protein complexes formed with NUP98-HOXA9. Mass spectrometry analysis identified that one metabolic regulator protein interacts with NUP98-HOXA9. The colony formation of the NUP98-HOXA9-immortalized mouse myeloid stem/progenitor cells was inhibited by shRNA and inhibitor for this metabolic protein. The inhibition was specific for NUP98 fusion-transformed cells. These results suggest that the metabolic protein can be a therapeutic target for NUP98 fusion-mediated leukemia.
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