| Project/Area Number |
24790999
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
膠原病・アレルギー・感染症内科学
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
KAWAKAMI Astushi 長崎大学, 大学院・医歯薬学総合研究科展開医療科学講座(第一内科), 教授 (90325639)
NAKAMURA Hideki 長崎大学, 病院第一内科, 講師 (10437832)
IWAMOTO Naoki 長崎大学, 病院第一内科, 助教 (80437897)
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| Co-Investigator(Renkei-kenkyūsha) |
KOGA Tomohiro 長崎大学, 病院第一内科, 助教 (90537284)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 全身性エリテマトーデス / ループス腎炎 / ポドサイト / CaMKIV / CD86 / 国際情報交換、アメリカ |
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| Research Abstract |
T cell from patients with systemic lupus erythematosus display increased expression of calcium/calmodulin kinase IV (CaMKIV). CaMKIV expression was found increased in podocytes of Lupus nephritis(LN) kidney biopsy specimens. The levels of nephrin and CaMKIV correlated in an inverse manner. Interestingly, culture of AB8/13 podocytes in the presence of IgG from LN sera led to 1.5-fold increase in the expression of CaMKIV mRNA. Gene array analysis revealed that the expression of genes related to the cell activation including CaMKIV increased significantly (P<0.05) and the regulation of neural precursor cell proliferation decreased significantly (P<0.01) in podocytes treated with IgG from LN patients. Our data demonstrate increased expression of CaMKIV in LN podocytes which may represent the result of exposure to IgG. We suspect that increased CaMKIV expression may contribute to the inability of podocytes to maintain the integrity of glomerular basement membrane in LN patients.
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