Effect of leptin as proinflammatory cytokine

• Project/Area Number: 24791008
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: 膠原病・アレルギー・感染症内科学
• Research Institution: Toho University
• Principal Investigator: KUSUNOKI Natsuko 東邦大学, 医学部, 博士研究員 (10328924)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: レプチン / 関節リウマチ / 炎症性サイトカイン / IL-6 / 線維芽細胞 / リウマチ学 / 炎症 / 滑膜線維芽細胞

Research Abstract

The aim of this study was to determine the influence of leptin on the production of proinflammatory cytokines by rheumatoid synovial fibroblasts (RSF). We detected leptin receptor mRNAs in RSFs. Expression of IL-6 mRNA was enhanced in a concentration-dependent manner by addition of leptin to RSF. IL-6 secretion by RSF showed an increase after leptin stimulation. A JAK2 inhibitor decreased leptin-induced IL-6 production. Enhanced phosphorylation of STAT3 was observed in RSF after stimulation by leptin. Leptin may be one of the proinflammatory cytokines that up-regulates IL-6 production in RSF via activation of JAK2/STAT3. Leptin and JAK/STAT pathway may represent a new alternative therapeutic target in the treatment of rheumatoid arthritis.

Research Products

• [Journal Article] Leptin stimulates interleukin-6 production via janus kinase 2/signal transducer and activator of transcription 3 in rheumatoid synovial fibroblasts (2013)
  • Author(s): Muraoka S., Kusunoki N., Takahasi H., Tsuchiyaa K., Kawai S
  • Journal Title: Clin Exp Rheumatol Volume: 31(4) Pages: 589-595
  • URL: http://www.clinexprheumatol.org/pubmed/find-pii.asp?pii=23622344
  • Peer Reviewed
• [Journal Article] Leptin stimulates interleukin-6 production via janus kinase 2/signal transducer and activator of transcription 3 in rheumatoid synovial fibroblasts. (2013)
  • Author(s): Muraoka S, Kusunoki N, Takahashi H, Tsuchiya K, Kawai S.
  • Journal Title: Clin Exp Rheumatol. Volume: 31(4) Pages: 589-595
  • Peer Reviewed
• [Presentation] JAK2/STAT3 is a major pathway of leptin-induced interleukin-6 production by rheumatoid synovial fibroblasts (2013)
  • Author(s): Muraoka S., Kusunoki N., Shikano K., Kaburaki M., Kitahara K., Tanaka N., Kaneko K., Yamamoto T., Kusunoki Y., Takagi K., Hasunuma T., Endo H., Kawai S
  • Organizer: Annual European Congress of Rheumatology EULAR 2013
  • Place of Presentation: FERIA DE MADRID (Madrid, Spain)
  • Year and Date: 2013-06-15
• [Presentation] レプチンはJAK-STAT 経路を介して関節リウマチ患者由来滑膜線維芽細胞のIL-6 産生を増加させる (2012)
  • Author(s): 村岡成, 楠夏子, 鎗田利香, 勝呂徹, 川合眞一
  • Organizer: 第33回日本炎症・再生医学会
  • Place of Presentation: ホテル日航福岡(福岡, 日本)
  • Year and Date: 2012-07-06
• [Presentation] Leptin は滑膜細胞のIL-6 産生を増加させる (2012)
  • Author(s): 村岡成, 楠夏子, 鎗田利香, 鹿野孝太郎, 鏑木誠, 北原加奈子, 金子開知, 田中菜穂子, 山本竜大, 楠芳恵, 高木賢治, 遠藤平仁, 勝呂徹, 川合眞一
  • Organizer: 第56回日本リウマチ学会総会・学術集会・第21回国際リウマチシンポジウム
  • Place of Presentation: グランドプリンスホテル新高輪(東京, 日本)
  • Year and Date: 2012-04-26
• [Presentation] Leptinは滑膜細胞のIL-6産生を増加させる
  • Author(s): 村岡成, 楠夏子, 鎗田利香, 鹿野孝太郎, 鏑木誠, 北原加奈子, 金子開知, 田中菜穂子, 山本竜大, 楠芳恵, 高木賢治, 遠藤平仁, 勝呂徹, 川合眞一
  • Organizer: 第56回日本リウマチ学会総会・学術集会・第21回国際リウマチシンポジウム
  • Place of Presentation: グランドプリンスホテル新高輪(東京都)
• [Presentation] レプチンはJAK-STAT経路を介して関節リウマチ患者由来滑膜線維芽細胞のIL-6産生を増加させる
  • Author(s): 村岡成, 楠夏子, 鎗田利香, 勝呂徹, 川合眞一
  • Organizer: 第33回日本炎症・再生医学会
  • Place of Presentation: ホテル日航福岡(福岡県)
• [Presentation] JAK2/STAT3 IS A MAJOR PATHWAY OF LEPTIN-INDUCED INTERLEUKIN-6 PRODUCTION BY RHEUMATOID SYNOVIAL FIBROBLASTS
  • Author(s): S. Muraoka, N. Kusunoki, K. Shikano, M. Kaburaki, K. Kitahara, N. Tanaka, K. Kaneko, T. Yamamoto, Y. Kusunoki, K. Takagi, T. Hasunuma, H. Endo, S. Kawai
  • Organizer: Annual European Congress of Rheumatology EULAR 2013
  • Place of Presentation: FERIA DE MADRID (Madrid, Spain)
• [Remarks] 東邦大学教育・研究業績データベース
  • URL: http://gyoseki.toho-u.ac.jp/thuhp/KgApp