| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Research Abstract |
SPACIA1 is a novel gene associated with abnormal synovial proliferation which is one of the major characteristics of rheumatoid arthritis (RA). In this study, we aimed to a precise understanding of the molecular mechanisms behind this process. We analyzed constituents of purified SPACIA1 protein complex using shotgun proteomics. We identified CDK6, one of functional genes at G1 phase, which gene expression is regulated by SPACIA1 in mRNA turnover. CDK6 knockdown by its siRNA inhibited the proliferation of cultured RA synovial fibroblasts. These results suggested that CDK6 is functionally involved in RA synoviocyte proliferation. Specific inhibition of CDK6 may be a useful therapeutic strategy for RA.
|
