| Project/Area Number |
24791020
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Infectious disease medicine
|
|---|
| Research Institution | Nagoya University |
|---|
Principal Investigator |
KIMURA Kouji 名古屋大学, 医学(系)研究科(研究院), 講師 (50425675)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Project Status |
Completed (Fiscal Year 2013)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Keywords | Klebsiella oxytoca / ベータラクタマーゼ阻害剤耐性 / ベータラクタマーゼRbiA / 等電点電気泳動法 / MLST / β-lactamase / RbiA |
|---|
| Research Abstract |
We analyzed clinical isolates of beta-lactamase inhibitor-resistant Klebsiella oxytoca, which suspected to spread nosocomially. The pulsed-field gel electrophoresis (PFGE) analysis showed that these clinical isolates have identical genetical background. The electrophoresis of isoelectric point suggested that the clinical isolate produced one kind of beta-lactamase. Moreover, we revealed that these clinical isolates produced a beta-lactamase RbiA, which we reported previously. We generated the E. coli producing a beta-lactamase RbiA by transformation. The E. coli showed beta-lactamase inhibitor-resistance, suggesting that producing a beta-lactamase RbiA is the major cause of beta-lactamase inhibitor-resistance in the clinical isolates of beta-lactamase inhibitor-resistant Klebsiella oxytoca.
|
