Investigation of infection of Staphylococcus aureus controlled by network for stringent regulation
| Project/Area Number |
24791029
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Infectious disease medicine
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | MRSA / バンコマイシン耐性 / 黄色ブドウ球菌 / 院内感染症 / slow-VISA / VISA / rpoB / バンコマイシン耐性黄色ブドウ球菌 / ppGpp / 緊縮制御ネットワーク / 緊縮応答 / 薬剤耐性 / 病原性 |
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| Outline of Final Research Achievements |
The Vancomycin(VCM)-intermediate Staphylococcus aureus (VISA) strains generally grow slowly, and form colonies on VCM-containing agar-plates after 48 h of incubation in population analysis. However, we have found a curious group of VISA strains whose colonies appear only after 72 h incubation. We designated them ‘slow-VISA’(sVISA) . As compared to extant VISA strains, sVISA strains had prolonged doubling-times and unstable resistance and growth phenotype. Whole genome sequencing of a representative sVISA strain Mu3-6R-P, which was obtained from hVISA strain Mu3 by selection with 6 mg/L of VCM, revealed a single mutation (R512P), in the rpoB gene encoding beta subunit of RNA polymerase (RNAP), as the responsible mutation for the sVISA phenotype . The sVISA strain had VCM MIC of 16 mg/L. However, after two days of drug-free passage, it generated large colonies, which showed hVISA phenotype carrying mutations (R512L)
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] "Slow VISA," a novel phenotype of vancomycin resistance, found in vitro in heterogeneous vancomycin-intermediate Staphylococcus aureus strain Mu3.2014
Author(s)
Saito M, Katayama Y, Hishinuma T, Iwamoto A, Aiba Y, Kuwahara-Arai K, Cui L, Matsuo M, Aritaka N, Hiramatsu K
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Journal Title
Antimicrob Agents Chemother
Volume: 58
Issue: 9
Pages: 5024-5035
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] 'Slow VISA' (sVISA), a novel phenotype of vancomycin resistance, obtained in vitro from hVISA strain Mu3.2014
Author(s)
Michie Saito, Yuki Katayama, Tomomi Hishinuma, Akira Iwamoto, Yoshifumi Aiba, Kyoko Kuwahara-Arai, Longzhu Cui, Miki Matsuo, Nanae Aritaka and Keiichi Hiramatsu.
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Journal Title
Antimicrobial Agents and Chemotherapy
Volume: In press
Related Report
Peer Reviewed
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[Journal Article] Genomic Basis for methicillin resistance in Staphylococcus aureus.2013
Author(s)
Keiichi Hiramatsu, Teruyo Ito, Sae Tsubakishita, Takashi Sasaki, Fumihiko Takeuchi, Yuh Morimoto, Yuki Katayama, Miki Matsuo, Kyoko Kuwahara, Tomomi Hishinuma, and Tadashi Baba.
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Journal Title
Infection and chemotherapy
Volume: 45
Pages: 117-136
NAID
Related Report
Peer Reviewed
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[Journal Article] Genomic Basis for ß-lactam Resistance in Staphylococcus aureus2013
Author(s)
Keiichi Hiramatsu, Teruyo Ito, Sae Tsubakishita, Takashi Sasaki, Fumihiko Takeuchi, Yuh Morimoto, Yuki Katayama, Miki Matsuo, Kyoko Kuwahara, Tomomi Hishinuma, and Tadashi Baba
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Journal Title
Infection and Chemotherapy (Korea)
Volume: submitted
Related Report
Peer Reviewed
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[Presentation] A new phenotypic resistance to vancomycin, slow-VISA2014
Author(s)
Yuki Katayama, Tomomi Hishinuma, Michie Sato, Akira Iwamoto, Yoshifumi Aiba Takashi Sasaki and Keiichi Hiramatsu
Organizer
Meeting in laboratory of Professor Henry, F. Chambers
Place of Presentation
University of California San Francisco
Year and Date
2014-08-30
Related Report
Invited
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