Analysis of chronic allergic inflammation using peripheral blood dendritic cells

• Project/Area Number: 24791040
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pediatrics
• Research Institution: Chiba University
• Principal Investigator: INOUE YUZABURO 千葉大学, 医学部附属病院, 助教 (00456063)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: アレルギー / 樹状細胞 / インフラマソーム / TSLP

Research Abstract

We analyzed differences of inflammasome activation or functions in myeloid dendritic cells (mDC) and plasmacytoid dendritic cells (pDC) in peripheral blood between allergic children and healthy controls. We found that LPS-induced caspase-1 activation was associated with decrease of TSLP receptor expression on mDC and increase of TSLP receptor on pDC, suggesting that increase of inflammasome activation might be associated with less Th2 induction and/or more Treg induction. In the patients who received rush oral immunotherapy for milk allergy, pDC was increased in peripheral blood, suggesting that pDCs might play a roll in the mechanisms of tolerance of food allergy.

Research Products

• [Presentation] Oral Immunotherapy for Milk Allergy Increases the Frequency of CD4+NKG2D+ T Cells, CD4+CD25-CD127- T Cells and Peripherally derived Tregs in Peripheral Blood (2013)
  • Author(s): Yuzaburo INOUE, et al
  • Organizer: Asia Pacific Congress of Allergy, Asthma and Clinical Immunology
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Changes in frequency of various regulatory T cells during rush oral immunotherapy for food allergy (2013)
  • Author(s): Yuzaburo INOUE, et al
  • Organizer: EAACI-WAO Congress 2013
  • Place of Presentation: Milan, Italy
• [Presentation] 食物アレルギーに対する急速経口免疫療法による種々の末梢血制御性T細胞の変化 (2013)
  • Author(s): 井上 祐三朗, 他
  • Organizer: 第25回日本アレルギー学会春季臨床大会
  • Place of Presentation: 横浜
• [Presentation] 気管支喘息の発症と増悪におけるウイルス感染の役割と治療 乳幼児喘息とウイルス感染 (2013)
  • Author(s): 井上 祐三朗
  • Organizer: 第25回日本アレルギー学会春季臨床大会
  • Place of Presentation: 横浜
• [Presentation] 気管支喘息の発症と増悪におけるウイルス感染の役割と治療 乳幼児喘息とウイルス感染 (2013)
  • Author(s): 井上祐三朗
  • Organizer: 第25回日本アレルギー学会春季臨床大会
  • Place of Presentation: 横浜
  • Invited
• [Presentation] Oral Immunotherapy for Milk Allergy Increases the Frequency of CD4+NKG2D+ T Cells, CD4+CD25-CD127- T Cells and Peripherally derived Tregs in Peripheral Blood (2013)
  • Author(s): Inoue Y, Chiba K, Kato I, Morita Y, Nakano T, Yamaide A, Suzuki S, Arima T, Tomiita M, Hoshioka A, Shimojo N, Kohno Y
  • Organizer: Asia Pacific Congress of Asthma, Allergy, and Clinical Immunology 2013
  • Place of Presentation: Taipei, Taiwan
• [Presentation] Relationship between thymic stromal lymphopoietin receptor expression on peripheral blood dendritic cells and caspase-1 activation in healthy children (2012)
  • Author(s): Yuzaburo INOUE, et al
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 大阪
• [Presentation] Association between regulation of thymic stromal lymphopoietin receptor expression on peripheral blood dendritic cells and LPS-induced inflammasome activation in healthy children (2012)
  • Author(s): Yuzaburo INOUE, et al
  • Organizer: 29th Symposium of the Collegium International Allergologicum
  • Place of Presentation: Jeju, Korea
• [Presentation] 全血LPS刺激による末梢血形質細胞様樹状細胞のThymic stromal lymphoprotein receptor 発現増加量は血清総IgEと逆相関する (2012)
  • Author(s): 井上 祐三朗, 他
  • Organizer: 第49回日本小児アレルギー学会
  • Place of Presentation: 大阪
• [Presentation] 全血LPS刺激による末梢血形質細胞様樹状細胞のThymic stromal lymphoprotein receptor発現増加量は血清総IgEと逆相関する (2012)
  • Author(s): 井上祐三朗 千葉浩輝 中野泰至 森田慶紀 有馬孝恭 下条直樹 河野陽一
  • Organizer: 第49回日本小児アレルギー学会
  • Place of Presentation: 大阪
• [Presentation] Association between regulation of thymic stromal lymphopoietin receptor expression on peripheral blood dendritic cells and LPS-induced inflammasome activation in healthy children (2012)
  • Author(s): Yuzaburo INOUE, Taiji NAKANO, Naoki SHIMOJO, Yoichi KOHNO
  • Organizer: 29th Symposium of the Collegium Internationale Allergologicum
  • Place of Presentation: Jeju, Korea
• [Presentation] Relationship between thymic stromal lymphopoietin receptor expression on peripheral blood dendritic cells and caspase-1 activation in healthy children (2012)
  • Author(s): Yuzaburo INOUE, Koki CHIBA, Taiji NAKANO, Yoshinori MORITA Takayasu Arima, Naoki SHIMOJO, Yoichi KOHNO
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 大阪