| Project/Area Number |
24791076
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatrics
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| Research Institution | Kagoshima University |
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Principal Investigator |
IRIE Rie 鹿児島大学, 医歯学域医学系, 助教 (90381178)
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| Research Collaborator |
Kosai Kenichiro 鹿児島大学, 医歯学域医学系 運動機能修復学講座 遺伝子治療・再生医学 分野, 教授 (90258418)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 脳・神経 / 脳・神経疾患 / 細胞・組織 / 生理活性 / 解剖学 / 超微形態学 / 脳神経疾患 / Rett / MeCP2 / 超微形態 |
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| Outline of Final Research Achievements |
The form change of a certain organ was observed in the MeCP2 gene deficiency mouse as a model mouse of Rett syndrome (RTT).I guessed that bioactive substance secretion abnormality was a cause.In this study,I tried the in vitro production using the cultured cell of the organ.I let I introduced the lentiviral vector which carried shRNA into a target cell, and the knockdown of the MeCP2 gene succeed. I performed the analysis of the property and the function of the MeCP2 knockdown cells and was able to duplicate bioactive secretion abnormality seen in in vivo.
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