Effect of early life stress on BDNF-mediated lipid homeostasis

• Project/Area Number: 24791111
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Embryonic/Neonatal medicine
• Research Institution: Kagawa University
• Principal Investigator: SUZUKI Shingo 香川大学, 医学部, 助教 (50451430)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: BDNF / コレステロール / 母子分離 / パルミトイル酸 / 母子分離モデル / パルミトレイン酸 / 神経細胞 / 発達 / シナプス

Research Abstract

Brain-derived neurotrophic factor (BDNF) mediates neuronal cholesterol biosynthesis during synapse development. However the effect of early life stress on the BDNF-dependent lipid homeostasis in neurons is not well understood. Thus, we here investigated the crosstalk between stress and BDNF signaling. First, it was shown that cholesterol biosynthesis in neurons were not affected by the treatment with corticosterone while stress hormones were known to inhibit cholesterol biosynthetic pathway in non-neuronal cells. Second, we found that maternal deprivation reduced the expression of cholesterol biosynthetic enzymes. These results indicated a possibility that early life stress influenced BDNF-mediated cholesterol biosynthesis by decreasing their expression.

Research Products

• [Journal Article] Early postnatal repeated maternal deprivation causes a transient increase in OMpg and BDNF in rat cerebellum suggesting precocious myelination (2014)
  • Author(s): Miki T, Yokoyama T, Kusaka T, Suzuki S, Ohta KI, Warita K, Wang ZY, Ueki M, Sumitani K, Bellinger FP, Tamai M, Liu JQ, Yakura T, Takeuchi Y
  • Journal Title: J Neurol Sci Volume: 336(1-2) Pages: 62-7
  • Peer Reviewed
• [Journal Article] Short-term ethanol exposure causes imbalanced neurotrophic factor allocation in the basal forebrain cholinergic system : a novel insight into understanding the initial processes of alcohol addiction (2014)
  • Author(s): Miki T, Kusaka T, Yokoyama T, Ohta KI, Suzuki S, Warita K, Jamal M, Wang ZY, Ueki M, Liu JQ, Yakura T, Tamai M, Sumitani K, Hosomi N, Takeuchi Y
  • Journal Title: J Neural Transm Volume: 121(2) Pages: 201-10
  • Peer Reviewed
• [Journal Article] Early postnatal repeated maternal deprivation causes a transient increase in OMgp and BDNF in rat cerebellum suggesting precocious myelination (2014)
  • Author(s): Miki T, Yokoyama T, Kusaka T, Suzuki S, Ohta K, Warita K, Wang ZY, Sumitani K, Bellinger FP, Tamai M, Liu JQ, Yakura T, Takeuchi Y
  • Journal Title: J Neurol Sci, J Neurol Sci Volume: 336 Issue: 1-2 Pages: 62-7
  • DOI: 10.1016/j.jns.2013.10.007
  • Peer Reviewed / Open Access
• [Journal Article] Prolonged maternal separation disturbs the serotonergic system during early brain development (2014)
  • Author(s): Ohta KI, Miki T, Warita K, Suzuki S, Kusaka T, Yakura T, Liu JQ, Tamai M, Takeuchi Y
  • Journal Title: Int J Dev Neurosci Volume: 33 Issue: 1 Pages: 15-21
  • DOI: 10.1016/j.ijdevneu.2013.10.007
  • Peer Reviewed / Open Access
• [Journal Article] Early postnatal maternal separation causes alterations in the expression of β3-adrenergic receptor in rat adipose tissue suggesting long-term influence on obesity (2013)
  • Author(s): Miki T, Liu JQ, Ohta K, Suzuki S, Kusaka T, Warita K, Yokoyama T, Jamal M, Ueki M, Yakura T, Tamai M, Sumitani K, Hosomi N, Takeuchi Y
  • Journal Title: Biochem Biophys Res Commun Volume: 442(1-2) Pages: 68-71
  • Peer Reviewed
• [Journal Article] Prolonged maternal separation disturbs the serotonergic system during early brain development (2013)
  • Author(s): Ohta KI, Miki T, Warita K, Suzuki S, Kusaka T, Yakura T, Liu JQ, Tamai M, Takeuchi Y
  • Journal Title: Int J Dev Neurosci Volume: 33C Pages: 15-21
  • Peer Reviewed
• [Journal Article] Gene expression of epigenetic regulatory factors related to primary silencing mechanism is less susceptible to lower doses of bisphenol A in embryonic hypothalamic cells (2013)
  • Author(s): Warita K, Mitsuhashi T, Ohta K, Suzuki S, Hoshi N, Miki T, Takeuchi Y
  • Journal Title: J Toxicol Sci Volume: 38(2) Pages: 285-9
  • NAID: 10031151432
  • Peer Reviewed
• [Journal Article] Immunohistochemical analysis of steroidogenic acute regulatory protein (StAR) and StAR-binding protein (SBP) expressions in the testes of mice during fetal development (2013)
  • Author(s): Warita K, Mitsuhashi T, Fukui S, Ohta K, Suzuki S, Miki T, Takeuchi Y, Yokoyama T, Kitagawa H, Sugawara T, Hoshi N
  • Journal Title: Reprod Biol Volume: 13(1) Pages: 92-5
  • Peer Reviewed
• [Journal Article] In vitro evaluation of gene expression changes for gonadotropin-releasing hormone 1, brain-derived neurotrophic factor, and neurotrophic tyrosine kinase, receptor, type 2, in response to bisphenol A treatment (2013)
  • Author(s): Warita K, Mitsuhashi T, Ohta KI, Suzuki S, Hoshi N, Miki T, Takeuchi Y
  • Journal Title: Congenit Anom (Kyoto) Volume: 53(1) Pages: 42-5
  • NAID: 50008814302
  • Peer Reviewed
• [Journal Article] Early postnatal maternal separation causes alterations in the expression ofβ3-adrenergic receptor in rat adipose tissue suggesting long-term influence on obesity (2013)
  • Author(s): Miki T, Liu JQ, Ohta K, Suzuki S, Kusaka T, Warita K, Yokoyama T, Jamal M, Ueki M, Yakura T, Tamai M, Sumitani K, Hosomi N, Takeuchi Y
  • Journal Title: Biochem Biophys Res Commun Volume: 442 Issue: 1-2 Pages: 68-71
  • DOI: 10.1016/j.bbrc.2013.11.005
  • Peer Reviewed
• [Journal Article] In vitro evaluation of gene expression changes for gonadotropin-releasing hormone 1, brain-derived neurotrophic factor, and neurotrophic tyrosine kinase, receptor, type 2, in response to bisphenol A treatment. (2013)
  • Author(s): Warita K, Mitsuhashi T, Ohta KI, Suzuki S, Hoshi N, Miki T, Takeuchi Y
  • Journal Title: Congenit Anom (Kyoto) Volume: 53(1) Pages: 42-5
  • NAID: 50008814302
  • Peer Reviewed
• [Journal Article] Elevated expression of brain-derived neurotrophic factor facilitates visual imprinting in chicks (2012)
  • Author(s): Suzuki K, Maekawa F, Suzuki S, Nakamori T, Sugiyama H, Kanamatsu T, Tanaka K, Ohki-Hamazaki H
  • Journal Title: J Neurochem Volume: 123(5) Pages: 800-10
  • Peer Reviewed
• [Journal Article] Morphological Evidence of an Altered Process of Synaptic Transcytosis in Adult Rats Exposed to Ethanol (2012)
  • Author(s): Takeuchi Y, Miki T, Liu JQ, Ohta KI, Warita K, Matsumoto Y, Suzuki S, Tamai M, Ameno K, Bedi KS, Yakura T
  • Journal Title: Alcohol Alcohol Volume: 47(6) Pages: 671-6
  • Peer Reviewed
• [Journal Article] BDNF-dependent accumulation of palmitoleic acid in CNS neurons (2012)
  • Author(s): Suzuki S, Hongli Q, Okada A, Kasama T, Ohta K, Warita K, Tanaka K, Miki T and Takeuchi Y
  • Journal Title: Cell Mol Neurobiol Volume: 32(8) Pages: 1367-73
  • Peer Reviewed
• [Journal Article] Microarray and gene ontology analyses reveal downregulation of DNA repair and apoptotic pathways in diethylstilbestrol-exposed testicular Leydig cells (2012)
  • Author(s): Warita K, Mitsuhashi T, Tabuchi Y, Ohta K, Suzuki S, Hoshi N, Miki T, Takeuchi Y
  • Journal Title: J Toxicol Sci Volume: 37(2) Pages: 287-95
  • NAID: 10030126622
  • Peer Reviewed
• [Journal Article] Neonatal repetitive maternal separation causes long-lasting alterations in various neurotrophic factor expression in the cerebral cortex of rats (2012)
  • Author(s): Lee KY, Miki T, Yokoyama T, Ueki M, Warita K, Suzuki S, Ohta KI, Wang ZY, Jamal M, Yakura T, Liu JQ, Hosomi N, Takeuchi Y
  • Journal Title: Life Sci. Life Sci Volume: 90(15-16) Pages: 578-84
  • Peer Reviewed
• [Journal Article] Morphological evidence of an altered process of (2012)
  • Author(s): Takeuchi Y, Miki T et al.
  • Journal Title: Alcohol Alcohol Volume: 47 Issue: 6 Pages: 671-6
  • DOI: 10.1093/alcalc/ags085
  • Peer Reviewed
• [Journal Article] BDNF-dependent accumulation of palmitoleic acid in CNS neurons. (2012)
  • Author(s): Suzuki S, Miki Tet al.
  • Journal Title: Cell Mol Neurobiol Volume: 32 Issue: 8 Pages: 1367-73
  • DOI: 10.1007/s10571-012-9863-x
  • Peer Reviewed
• [Journal Article] Neonatal repetitive maternal separation causes long-lasting alterations in various neurotrophic factor expression in the cerebral cortex of rats. (2012)
  • Author(s): Lee KY, Miki T et al.
  • Journal Title: Life Sci. Volume: 90 Issue: 15-16 Pages: 578-584
  • DOI: 10.1016/j.lfs.2012.01.021
  • Peer Reviewed
• [Presentation] ニューロンによるコレステロール合成とシナプス発達 (2013)
  • Author(s): 鈴木辰吾
  • Organizer: 香川脳神経化学研究会
  • Year and Date: 2013-01-18
• [Presentation] 神経回路の新しい可視化法の開発 (2013)
  • Author(s): 鈴木辰吾
  • Organizer: 包括的神経グリア研究会
  • Year and Date: 2013-01-12
• [Presentation] 「BDNFによるニューロンのコレステロール合成誘導とプレシナプス蛋白質のラフトへの移動 (2013)
  • Author(s): 鈴木辰吾
  • Organizer: 香川脳神経化学研究会
  • Place of Presentation: 高松
• [Presentation] BDNF-regulared cholesterol metabolism for synapse development (2012)
  • Author(s): Suzuki S
  • Organizer: The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry
• [Presentation] BDNF-regulared cholesterol metabolism for synapse development (2012)
  • Author(s): Shingo SUZUKI
  • Organizer: The 11th Biennial Meeting of the Asian Pacific Society for Neurochemistry
  • Place of Presentation: 神戸