Fecal microflora composition change in neonates with intensive theraphy

Research Project

Project/Area Number	24791120
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Embryonic/Neonatal medicine
Research Institution	Kitasato University
Principal Investigator	ITO Takashi 北里大学, 生命科学研究所, 研究員 (90383629)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2014: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000) Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords	腸内細菌叢 / 新生児 / 早産児 / 腸内細菌 / NICU / 感染
Outline of Final Research Achievements	The composition of microflora may be influenced by various factors, such as nutrition and antibiotics treatment. The aim of this study is to understand the sequential change of microbial flora in the feces from preterm baby through 16S rRNA PCR assay. The fecal samples were obtained from 33 infants(31.6±0.5weeks gestation, 1398±83g birth body weight ). After bacterial DNA was extracted, subjected to PCR amplification. The result was that supplement of antibiotics did not influence the distribution of each bacterial species. In the group of probiotics treatment and the group of breast milk only, the colony of Bifidobacterium was found at very early postnatal day, and S.aureus was not colonized until the first week of life in the group of breast milk. In this study, probiotics and breast milk may work to compose the balanced microflora for preterm infants in NICU, long term observation is required to determine if this contribute to adverse outcome in future.