A development of easy-to-use method for diagnosis of scabies by DNA amplification and identification of drug-tolerant scabies for antiscabietic drug

• Project/Area Number: 24791131
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Hirosaki University
• Principal Investigator: MINAKAWA Satoko 弘前大学, 医学部附属病院, 医員 (20436033)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 疥癬 / ヒゼンダニ / 遺伝子 / LAMP法

Research Abstract

We have to remember the scabies as highly infectious disease. The purpose of this study is a development of easy-to-use method for diagnosis of scabies by DNA amplification. The DNA extracted from scabies's bodies and eggs was used as a template. We researched by DNA sequencing of the polymerase chain reaction (PCR) products and Loop-Mediated Isothermal Amplification(LAMP). We examined the patients with highly pruritic red papules, their family and all healthcare professionals in charge of the patients after physical examination.

Research Products

• [Journal Article] Mutational analysis on 16 Japanese population cases with epidermolysis bullosasimplex (2013)
  • Author(s): Minakawa S, Nakano H, Nakajima K, Matsuzaki Y, Takiyoshi N, Akasaka E,Rokunohe D, Sawamura D
  • Journal Title: J Dermatol Sci Volume: 72(pii: S0923-1811(13)00275-2) Pages: 330-332
  • Peer Reviewed
• [Journal Article] Tegafur/gimeracil/oteracil (TS-1) induced StevenseJohnson syndrome: Case report. (2013)
  • Author(s): Minakawa S, Matsuzaki Y, Nakajima K, Nakano H, Sawamura D
  • Journal Title: Dermatol Sinica Volume: 31 Issue: 3 Pages: 154-156
  • DOI: 10.1016/j.dsi.2012.12.005
  • Peer Reviewed
• [Journal Article] EPR spectroscopic investigation ofpsoriatic finger nails (2013)
  • Author(s): Nakagawa K, Minakawa S, Sawamura D
  • Journal Title: Skin Res Technol Volume: 19(4) Issue: 4 Pages: 450-453
  • DOI: 10.1111/srt.12068
  • Peer Reviewed
• [Journal Article] Mutational analysis on 16 Japanese population cases with epidermolysis bullosasimplex (2013)
  • Author(s): Minakawa S, Nakano H, Nakajima K, Matsuzaki Y, Takiyoshi N, Akasaka E, Rokunohe D, Sawamura D
  • Journal Title: J Dermatol Sci Volume: 72(3) Issue: 3 Pages: 330-332
  • DOI: 10.1016/j.jdermsci.2013.08.001
  • Peer Reviewed
• [Journal Article] Q-switched ruby laser therapy and long-term follow-up evaluation of small to medium-sized congenital melanocyticnaevi (2012)
  • Author(s): Minakawa S, Takeda H, Korekawa A, Kaneko T, Urushidate, Sawamura D
  • Journal Title: Clin Exp Dermatol Volume: 37 Issue: 4 Pages: 438-40
  • DOI: 10.1111/j.1365-2230.2011.04226.x
  • Peer Reviewed
• [Journal Article] Acantholysis caused repeated hemorrhagic bullae in a case of acantholytic acanthoma. (2012)
  • Author(s): Minakawa S, Matsuzaki Y, Nakano H, Sawamura D, Elenitsas R.
  • Journal Title: J Dermatol. Volume: 39 Issue: 12 Pages: 1107-1108
  • DOI: 10.1111/j.1346-8138.2012.01564.x
  • Peer Reviewed
• [Journal Article] A Case of Erythema Nodosum Associated with Subareolar Abscess. (2012)
  • Author(s): Minakawa S
  • Journal Title: Journal of Cosmetics, Dermatological Sciences and Applications Volume: 2 Issue: 04 Pages: 263-264
  • DOI: 10.4236/jcdsa.2012.24049
  • Peer Reviewed
• [Journal Article] Histologic features of nail cosmetics (2012)
  • Author(s): Anolik RB, Elenitsas R, Minakawa S, Nguyen J, Rubin AI
  • Journal Title: Am J Dermatopathol Volume: 34 Issue: 4 Pages: 412-5
  • DOI: 10.1097/dad.0b013e3182377005
  • Peer Reviewed
• [Journal Article] Spectroscopic Evidence of Abnormal sSructure of Psoriasis Vulgaris Stratum Corneum (2012)
  • Author(s): K. Nakagawa, S. Minakawa, and D. Sawamura
  • Journal Title: Journal of Dermatological Science Volume: 65(3) Issue: 3 Pages: 222-224
  • DOI: 10.1016/j.jdermsci.2012.01.002
  • Peer Reviewed
• [Journal Article] Angiolymphoid hyperplasia with eosinophilia in infancy. (2012)
  • Author(s): Korekawa A
  • Journal Title: J Dermatol. Volume: 39 Issue: 12 Pages: 1052-1054
  • DOI: 10.1111/j.1346-8138.2012.01578.x
  • Peer Reviewed
• [Presentation] 即時型インスリンアレルギーと考えられた1例 (2013)
  • Author(s): 皆川智子,金子高英,中野創,澤村大輔,森山貴子,森本武史
  • Organizer: 日本皮膚科学会青森地方会第364回例会
  • Place of Presentation: 弘前大学医学部コミュニケーションセンター(弘前市)
  • Year and Date: 2013-11-24
• [Presentation] 単純型表皮水疱症の1例 (2013)
  • Author(s): 皆川智子,中島康爾,中野創,澤村大輔,千葉純子
  • Organizer: 日本皮膚科学会青森地方会第363回例会
  • Place of Presentation: 八戸パークホテル(八戸市)
  • Year and Date: 2013-08-25
• [Presentation] 家族性良性慢世天疱瘡の1例 (2013)
  • Author(s): 皆川智子,松﨑康司,金子高英,中野創,澤村大輔,三橋善比古
  • Organizer: 第40回皮膚かたち研究会
  • Place of Presentation: 石垣記念ホール(東京)
  • Year and Date: 2013-07-28
• [Presentation] Stevens -Johnson syndrome associated with TS-1 (2013)
  • Author(s): Minakawa S, Matsuzaki Y, Nakajima K, Nakano H, Sawamura D
  • Organizer: 71th AAD Annual Meeting
  • Place of Presentation: Florida(America)
  • Year and Date: 2013-03-02
• [Presentation] 単純型表皮水疱症 16例の遺伝子診断 (2013)
  • Author(s): 皆川智子,中野 創,中島康爾,澤村大輔,池田志斈,玉井克人,三橋善比古
  • Organizer: 第77回日本皮膚科学会東部支部学術大会
  • Place of Presentation: 大宮ソニックシティ(さいたま市)
• [Presentation] 即時型インスリンアレルギーと考えられた1例 (2013)
  • Author(s): 皆川智子、金子高英、中野 創、澤村大輔、森山貴子、森本武史
  • Organizer: 日本皮膚科学会青森地方会第364回例会
  • Place of Presentation: 弘前大学医学部コミュニケーションセンター
• [Presentation] 単純型表皮水疱症16例の遺伝子診断.第77回日本皮膚科学会東部支部学術大会、2013.9.21-22.大宮ソニックシティ（さいたま市） (2013)
  • Author(s): 皆川智子、中野 創、中島康爾、澤村大輔、池田志斈、玉井克人、三橋善比古
  • Organizer: 第77回日本皮膚科学会東部支部学術大会
  • Place of Presentation: 大宮ソニックシティ（さいたま市）
• [Presentation] 単純型表皮水疱症の 1例. (2013)
  • Author(s): 皆川智子、中島康爾 、中野 創、千葉純子 、澤村大輔
  • Organizer: 第363回日本皮膚科学会青森地方会
  • Place of Presentation: 八戸パークホテル
• [Presentation] 家族性良性慢世天疱瘡の1例. (2013)
  • Author(s): 皆川智子, 松﨑康司, 金子高英, 中野 創, 澤村大輔, 三橋善比古
  • Organizer: 第40 回皮膚かたち研究会
  • Place of Presentation: 石垣記念ホール（東京）
• [Presentation] Stevens - Johnson syndrome associated with TS-1 (2013)
  • Author(s): Minakawa S
  • Organizer: 71th AAD Annual Meeting
  • Place of Presentation: Miami Beach, Fla.(アメリカ）
• [Presentation] ウステキヌマブで改善した乾癬の2例 (2012)
  • Author(s): 皆川智子,金子高英,松崎康司,中野創,澤村大輔,木村淳也
  • Organizer: 日本皮膚科学会青森地方会第359回例会
  • Place of Presentation: ホテルニューキャッスル(弘前市)
  • Year and Date: 2012-09-02
• [Presentation] 有棘細胞癌,Bowen病,日光角化症を伴った眼皮膚白皮症の1例 (2012)
  • Author(s): 皆川智子,金子高英,松﨑康司,中野創,澤村大輔,三上誠,鈴木民夫,三橋善比古
  • Organizer: 第39回皮膚かたち研究学会
  • Place of Presentation: 東京慈恵会医科大学(東京)
  • Year and Date: 2012-07-29
• [Presentation] 有棘細胞癌、Bowen病、日光角化症を伴った眼皮膚白皮症の1例
  • Author(s): 皆川智子
  • Organizer: 第39回皮膚かたち研究学会
  • Place of Presentation: 東京慈恵会医科大学（東京都）
• [Presentation] ウステキヌマブで改善した乾癬の2例
  • Author(s): 皆川智子
  • Organizer: 日本皮膚科学会青森地方会第359回例会
  • Place of Presentation: ホテル青森（青森県）