Influence of RORgammat expression in CD4+ Tcell induction

• Project/Area Number: 24791134
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: University of Tsukuba
• Principal Investigator: Fujisawa Yasuhiro 筑波大学, 医学医療系, 講師 (70550193)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2015: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: Th17細胞 / RORgammat / 接触皮膚炎 / RORγt / Th17 / Neuropilin-1 / Treg

Outline of Final Research Achievements

Mice transplanted with bone marrow stem cells transduced with RORgammat which controls intreleukin-17 producing T cells (Th17) showed reduced contact hypersensitivity reaction. Regulatory T cells (treg) might have some role in this phenotype and indeed, the number of Treg was increased in these mice. On the other hand, these mice developed vitiligo, alopecia, and rectal prolapse after long-term breeding. These phenotypes are considered to be an autoimmune disease and conflict to the result of contact hypersensitivity. Thus, next we attempted to make neuropilin-1 transgenic mice to investigate correlation between Treg activity and these phenotypes. However, we could not get litter mate.