Development of highly sensitive and rapid ELISA using luminometer and its application to detect autoantibodies in dermatomyositis
| Project/Area Number |
24791150
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Nagoya University |
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Principal Investigator |
SAWADA Masaki 名古屋大学, 医学部附属病院, 助教 (80467315)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 皮膚筋炎 / 自己抗体 / ELISA / リコンビナントタンパク |
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| Research Abstract |
Although recently identified dermatomyositis-associated autoantibodies are very useful in clinics,they can be identified by a very minor population of laboratory. Moreover, immunoprecipitation methods used in the laboratories cannot produce results in a short time. We have developed technically easy experimental methods using in vitro translation and transcription system to detect autoantibodies; the preparation of plasmids harboring cDNA in interest->expression of biotinylated recombinant protein by a commercial kit->captured ELISA with streptavidin-coated plates. This is an epoch-making method which can be constructed for approximate one week after getting a cDNA inserted into a vector with T7 promotor.
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Report
(3 results)
Research Products
(23 results)
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[Journal Article] Clinicopathologic analysis of 66 Japanese thin melanomas with metastasis of sentinel or regional lymph node2013
Author(s)
Mori M, Sugiura M, Kono M, Matsumoto T, Sawada M, Yokota K, Yasue S, Shibata S, Sakakibara A, Nakamura S, Tomita Y, Akiyama M
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Journal Title
J Cutan Pathol
Volume: 40
Pages: 1027-34
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[Journal Article] Clinico-pathologic Analysis of 66 Japanese Thin Melanomas with Metastasis of Sentinel or Regional Lymph Node.2013
Author(s)
Mori M, Sugiura M, Kono M, Matsumoto T, Sawada M, Yokota K, Yasue S, Shibata S, Sakakibara A, Nakamura S, Tomita Y, Akiyama M.
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Journal Title
J Cutan Pathol
Volume: 40
Issue: 12
Pages: 1027-34
DOI
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Peer Reviewed
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[Presentation] メルケル細胞癌の3例2012
Author(s)
澤田 昌樹, 杉浦 一充, 横田 憲二, 松本 高明, 武市 拓也, 長坂 徹郎, 片野晴隆, 秋山 真志
Organizer
第111回日本皮膚科学会総会
Place of Presentation
京都, 京都府
Year and Date
2012-06-02
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