| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Research Abstract |
Although recently identified dermatomyositis-associated autoantibodies are very useful in clinics,they can be identified by a very minor population of laboratory. Moreover, immunoprecipitation methods used in the laboratories cannot produce results in a short time. We have developed technically easy experimental methods using in vitro translation and transcription system to detect autoantibodies; the preparation of plasmids harboring cDNA in interest->expression of biotinylated recombinant protein by a commercial kit->captured ELISA with streptavidin-coated plates. This is an epoch-making method which can be constructed for approximate one week after getting a cDNA inserted into a vector with T7 promotor.
|
