Role of phospholipase C epsilon in Ultaviolet induced skin carcinogenesis, skin inflamation, cataract, and psoraiasis

• Project/Area Number: 24791155
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Kobe University
• Principal Investigator: SAKAGUCHI Masanobu 神戸大学, 医学部附属病院, 助教 (30622881)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: PLCε / 皮膚炎症 / 皮膚発癌 / 乾癬 / ホスホリパーゼＣε / 紫外線 / アポトーシス / 白内障

Outline of Final Research Achievements

In order to examine the role of phospholipase C epsilon (PLCε) in the skin, we created transgenic mice overexpressing PLCε in epidermal keratinocytes(K5-PLCε-TG mouse). The K5-PLCε-TG mice spontaneously developed skin inflammation as characterized by formation of adherent silvery scales. Histologically, acanthuses, neutrophil infiltration in the epidermis, and STAT3 activation in keratinocytes were observed.

Research Products

• [Journal Article] Diffuse plane normolipemic xanthoma associated with chronic myelomonocytic leukemia-1. (2014)
  • Author(s): Oka, M., Okamura, A., Kawano, S., Fukumoto, T., Sakaguchi, M., Nishigori, C.
  • Journal Title: Eur. J. Dermatol. Volume: 24 Issue: 1 Pages: 112-113
  • DOI: 10.1684/ejd.2013.2256
  • Peer Reviewed
• [Journal Article] Three Cases of Linear IgA/IgG Bullous Dermatosis Showing IgA and IgG Reactivity With Multiple Antigens, Particularly Laminin-332 (2013)
  • Author(s): Sakaguchi M, Bito T, Oda Y, Kikusawa A, Nishigori C, Munetsugu T, Yokozeki H, Itotani Y, Niguma T, Tsuruta D, Tateishi C, Ishii N, Koga H, Hashimoto T
  • Journal Title: JAMA Dermatol Volume: 149(11) Issue: 11 Pages: 1308-1313
  • DOI: 10.1001/jamadermatol.2013.5691
  • Peer Reviewed
• [Journal Article] Role and Regulation of STAT3Phosphorylation at Ser727in Melanocytes and Melanoma Cells (2012)
  • Author(s): Sakaguchi, M., Oka, M., Iwasaki, T., Fukami, Y., Nishigori, C.
  • Journal Title: J Invest Dermatol Volume: (掲載確定) Issue: 7 Pages: 1877-1885
  • DOI: 10.1038/jid.2012.45
  • Peer Reviewed
• [Journal Article] Peristomal skin ulcer with intestinal metaplasia. (2012)
  • Author(s): Ono, R., Oka, M., Sakaguchi, M.,Nishioka, E., Kawakami, F., Nagano, T., Kunisada, M., Nishigori, C.
  • Journal Title: Br. J. Dermatol. Volume: 167 Issue: 1 Pages: 204-206
  • DOI: 10.1111/j.1365-2133.2012.10819.x
  • Peer Reviewed
• [Presentation] 外陰部病変および外陰外病変を有し、かつ、口腔粘膜に扁平苔癬を合併した硬化性萎縮性苔癬の1例 (2014)
  • Author(s): 南 知里、福本 毅、坂口 正展、竹内 聖二、福永 淳、岡 昌宏、高橋 佑輔、古森 孝英、植田 透子、錦織 千佳子
  • Organizer: 日本皮膚科学会中部支部学術大会
  • Place of Presentation: 大阪
  • Year and Date: 2014-10-25 – 2014-10-26
• [Presentation] Ultraviolet B-Induced Up-Regulation Of MCL-1L Mediated By The MEK-ERK1/2-STAT3 Signaling Pathway has a protective role in Apoptosis in Melanocytes (2014)
  • Author(s): Takeshi Fukumoto, Masahiro Oka, Masanobu Sakaguchi, Tetsushi Iwasaki, Takanori Hashimoto, Youbin Moon, Yasuo Fukami, Chikako Nishigori
  • Organizer: XVII International pigment cell conference
  • Place of Presentation: Singapore
  • Year and Date: 2014-09-04 – 2014-09-07
• [Presentation] Role and regulation of STAT3 phosphorylation at Ser727 in melanocytes and melanoma cells. (2012)
  • Author(s): 坂口正展、岡昌宏、福本毅、岩崎哲史、深見泰夫、錦織千佳子
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ホテルロイトン札幌
• [Presentation] Role and regulation of STAT3 phosphorylation at Ser727 in melanocytes and melanoma cells. (2012)
  • Author(s): Sakaguchi, M., Oka, M., Iwasaki, T., Fukami, Y., Nishigori, C.
  • Organizer: The 37th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: ロワジールホテル＆スパタワー那覇