The effect of aberrant expression of kallikrein 7 induced by Th2 cytokines on epidermal barrier

• Project/Area Number: 24791157
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Okayama University
• Principal Investigator: MORIZANE Shin 岡山大学, 大学病院, 助教 (80423333)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: アトピー性皮膚炎 / セリンプロテアーゼ / カリクレイン / Tｈ２サイトカイン

Research Abstract

TNF-alpha and IL-17A significantly up-regulated the expression of LEKTI, SLPI, and elafin in cultured normal human epidermal keratinocytes. Immunohistochemical analysis confirmed LEKTI expression in atopic dermatitis lesions was almost same as in normal skin, but it was significantly higher in psoriasis lesions than in atopic dermatitis lesions.
Topical application of serine protease inhibitors such as benzamidine did not significantly improve the skin lesions in a mouse model of Th2-dependent dermatitis.

Research Products

• [Journal Article] Th2 サイトカインはアトピー性皮膚炎患者におけるカリクレイン7 の発現と機能を増強する (2013)
  • Author(s): 森実真
  • Journal Title: 岡山医学会雑誌(0030-1558) Volume: 125巻3号 Pages: 217-220
  • NAID: 130004505789
• [Journal Article] 【アトピー性皮膚炎の発症とバリア機能】 皮膚の炎症と表皮バリア機能(解説/特集) (2013)
  • Author(s): 森実真
  • Journal Title: アレルギーの臨床(0285-6379) Volume: 33巻2号 Pages: 142-146
• [Journal Article] 【アトピー性皮膚炎の発症とバリア機能】 皮膚の炎症と表皮バリア機能(解説/特集) (2013)
  • Author(s): 森実真
  • Journal Title: アレルギーの臨床 Volume: 33 Pages: 142-146
• [Journal Article] 皮膚の炎症と表皮バリア機能 (2013)
  • Author(s): 森実真
  • Journal Title: アレルギーの臨床 Volume: 33 Pages: 142-146
• [Journal Article] TH2 cytokines increase kallikrein 7 expression and function in patients with atopic dermatitis (2012)
  • Author(s): Morizane S, Yamasaki K, Kajita A, Ikeda K, Zhan M, Aoyama Y, Gallo RL, Iwatsuki K
  • Journal Title: J Allergy Clin Immunol Volume: 130(1) Pages: 259-61
  • NAID: 120005357035
  • Peer Reviewed
• [Journal Article] IL-4 and IL-12 Polymorphisms are Associated with Response to Suplatast Tosilate, a Th2 Cytokine Inhibitor Inhibitor, in Patients with Atopic Dermatitis (2012)
  • Author(s): H Nagase, Y Nakachi, K Ishida, M Kiniwa, S Takeuchi, I Katayama, Y Matsumoto, F Furukawa, S Morizane, S Kaneko, Y Tokura, M Takenaka, Y Hatano and Y Miyachi
  • Journal Title: The Open Dermatology Journal Volume: 6 Pages: 42-50
  • Peer Reviewed
• [Journal Article] TH2 cytokines increase kallikrein 7 expression and function in patients with atopic dermatitis (2012)
  • Author(s): Morizane, S., Yamasaki, K., Kajita, A., Ikeda, K., Zhan, M., Aoyama, Y., Gallo, R. L., and Iwatsuki, K.
  • Journal Title: J Allergy Clin Immunol Volume: 130 Issue: 1 Pages: 259-261
  • DOI: 10.1016/j.jaci.2012.03.006
  • NAID: 130004505789
  • Peer Reviewed
• [Presentation] アトピー性皮膚炎の治療を考える~TSLP の発現に与える影響~ (2013)
  • Author(s): 森実真
  • Organizer: 第10回小児皮膚・疾患フォーラム
  • Place of Presentation: 岡山
  • Year and Date: 2013-06-26
• [Presentation] Th2 サイトカインはアトピー性皮膚炎患者におけるカリクレイン7 の発現と機能を増強する (2013)
  • Author(s): 森実真
  • Organizer: 第112回岡山医学会総会
  • Place of Presentation: 岡山
  • Year and Date: 2013-06-01
  • Invited
• [Presentation] Inflammatory cytokines regulate expression of lympho-epithelial Kazal-type-related inhibitor in epidermal keratinocytes (2013)
  • Author(s): S. Morizane, K. Iwatsuki
  • Organizer: 5th International Symposium on Kallikreins and Kallikrein-Related Peptidases
  • Place of Presentation: トロント, カナダ
• [Presentation] Inflammatory Cytokines Regulates Expression of Lympho-Epithelial Kazal-type-related Inhibitor in Epidermal Keratinocytes (2013)
  • Author(s): Shin Morizane, Keiji Iwatsuki
  • Organizer: 5th International Symposium on Kallikreins and Kallikrein-Related Peptidases
  • Place of Presentation: トロント，カナダ
• [Presentation] Clinical treatments for atopic dermatitis modulate thymic stromal lympopoietin expression in keratinocytes (2012)
  • Author(s): K Ikeda, S Morizane, Y Aoyama, K Iwatsuki
  • Organizer: The 37th Annual Meeting of the Japanese Society for Investigative Dermatology
  • Place of Presentation: Okinawa, Japan
• [Presentation] Th2サイトカインはアトピー性皮膚炎患者における組織カリクレイン７の発現および機能を増強する
  • Author(s): 森実真
  • Organizer: 第１１２回岡山医学会総会
  • Place of Presentation: 岡山
  • Invited