Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Research Abstract |
We detected LYVE-1 cleavage quantified by generating a fusion protein conjugating alkaline phosphatase (AP) to the N-terminal region of LYVE-1. This fusion protein was expressed in LECs by using an adenovirus vector. After IL-1Beta stimulation, AP activity was detected in the culture supernatant,indicating that LYVE-1 was shed into the supernatant. Furthermore, we elucidated IL-1Beta sheds LYVE-1 in LECs via the activation of MAPKs and ADAMs.
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