Induction of LYVE-1 shedding in cultured human lymphatic endothelial cells
| Project/Area Number |
24791160
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Ehime University |
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Principal Investigator |
OKAZAKI Hidenori 愛媛大学, 医学(系)研究科(研究院), 助教 (90622382)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | リンパ管内皮細胞 / ectodomain shedding / I型膜タンパク / LYVE-1 |
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| Research Abstract |
We detected LYVE-1 cleavage quantified by generating a fusion protein conjugating alkaline phosphatase (AP) to the N-terminal region of LYVE-1. This fusion protein was expressed in LECs by using an adenovirus vector. After IL-1Beta stimulation, AP activity was detected in the culture supernatant,indicating that LYVE-1 was shed into the supernatant. Furthermore, we elucidated IL-1Beta sheds LYVE-1 in LECs via the activation of MAPKs and ADAMs.
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Report
(3 results)
Research Products
(7 results)
