Induction of LYVE-1 shedding in cultured human lymphatic endothelial cells

• Project/Area Number: 24791160
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Dermatology
• Research Institution: Ehime University
• Principal Investigator: OKAZAKI Hidenori 愛媛大学, 医学(系)研究科(研究院), 助教 (90622382)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: リンパ管内皮細胞 / ectodomain shedding / I型膜タンパク / LYVE-1

Research Abstract

We detected LYVE-1 cleavage quantified by generating a fusion protein conjugating alkaline phosphatase (AP) to the N-terminal region of LYVE-1. This fusion protein was expressed in LECs by using an adenovirus vector. After IL-1Beta stimulation, AP activity was detected in the culture supernatant,indicating that LYVE-1 was shed into the supernatant. Furthermore, we elucidated IL-1Beta sheds LYVE-1 in LECs via the activation of MAPKs and ADAMs.

Research Products

• [Journal Article] Eccrine sweat contains IL- 1α, IL-1β and IL-31 and activates epidermal keratinocytes as a danger signal (2013)
  • Author(s): Dai X, Okazaki H, Hanakawa Y, Murakami M, Tohyama M, Shirakata Y, Sayama K
  • Journal Title: PLoS One Volume: 8
  • Peer Reviewed
• [Journal Article] Eccrine sweat contains IL-1α, IL-1β and IL-31 and activates epidermal keratinocytes as a danger signal. (2013)
  • Author(s): Dai X, Okazaki H, Hanakawa Y, Murakami M, Tohyama M, Shirakata Y, Sayama K.
  • Journal Title: PLoS One. Volume: ８ Issue: 7 Pages: 1-8
  • DOI: 10.1371/journal.pone.0067666
  • Peer Reviewed
• [Journal Article] Interactions between myofibroblast differentiation and epidermogenesis in constructing human living skin equivalents (2012)
  • Author(s): Yang L, Hashimoto K, Tohyama M, Okazaki H, Dai X, Hanakawa Y, Sayama K, Shirakata Y
  • Journal Title: J Dermatol Sci Volume: 65 Pages: 50-7
  • Peer Reviewed
• [Journal Article] Targeted overexpression of Angptl6/angiopoietinrelated growth factor in the skin promotes angiogenesis and lymphatic vessel enlargement in response to ultraviolet B (2012)
  • Author(s): Okazaki H, Hirakawa S, Shudou M, Nakaoka Y, Shirakata Y, Miyata K, Oike Y, Hashimoto K, Sayama K
  • Journal Title: J Dermatol Volume: 39 Pages: 366-74
  • Peer Reviewed
• [Presentation] Autophagy is an innate immune system of epidermal keratinocytes (2012)
  • Author(s): Okazaki H
  • Organizer: SID
  • Place of Presentation: North Carolina
• [Presentation] Intracellular dsRNA activates inflammasome in the keratinocytes resulting in the release of IL-18 (2012)
  • Author(s): Dai X, Okazaki H
  • Organizer: SID
  • Place of Presentation: North Carolina
• [Presentation] Autophagy is induced by differenciation in epidermal keratinocytes (2012)
  • Author(s): Dai X, Okazaki H
  • Organizer: 42nd ESDR
  • Place of Presentation: Venice