Induction of LYVE-1 shedding in cultured human lymphatic endothelial cells
| Project/Area Number | 24791160 |
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Dermatology
|
|---|
| Research Institution | Ehime University |
|---|
Principal Investigator |
OKAZAKI Hidenori 愛媛大学, 医学(系)研究科(研究院), 助教 (90622382)
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31(Estimated)
|
| Project Status |
Completed(Fiscal Year 2013)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost : ¥3,200,000、Indirect Cost : ¥960,000)
Fiscal Year 2013 : ¥1,820,000 (Direct Cost : ¥1,400,000、Indirect Cost : ¥420,000)
Fiscal Year 2012 : ¥2,340,000 (Direct Cost : ¥1,800,000、Indirect Cost : ¥540,000)
|
|---|
| Keywords | リンパ管内皮細胞 / ectodomain shedding / I型膜タンパク / LYVE-1 |
|---|
| Research Abstract |
We detected LYVE-1 cleavage quantified by generating a fusion protein conjugating alkaline phosphatase (AP) to the N-terminal region of LYVE-1. This fusion protein was expressed in LECs by using an adenovirus vector. After IL-1Beta stimulation, AP activity was detected in the culture supernatant,indicating that LYVE-1 was shed into the supernatant. Furthermore, we elucidated IL-1Beta sheds LYVE-1 in LECs via the activation of MAPKs and ADAMs.
|
Report
(3results)
Research Products
(7results)
