| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Outline of Final Research Achievements |
We synthesized 6 types of CRISPR/Cas9-Mitf corresponding to the Mitf sequence which is involved in melanin synthesis as well as differentiation of melanocyte and melanoma, and transfected them into human melanoma cell lines. CRISPR/Cas9-Mitf-4-mediated knockout of Mitf suppressed viability of HMV-II melanoma by 13% in comparison with control HMV-II melanoma. Low expression of Mitf mRNA in control amelanotic human melanoma SK-Mel 28 and in control melanotic human melanoma G361, and high expression of Mitf mRNA in control melanotic human melanoma HMV-II were confirmed. CRISPR/Cas9-mediated knockout of Mitf might suppress growth of certain types of human melanoma. While Mitf mRNA expression levels in human melanoma might not be associated with melanin production ability.
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