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Development of therapeutic RNA interference of human melanoma for the purpose of database design

Research Project

Project/Area Number 24791168
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Dermatology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

NAKAI Noriaki  京都府立医科大学, 医学(系)研究科(研究院), 講師 (80453108)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywordsメラノーマ / 分子治療 / Mitf / ヒト / 遺伝子治療 / 悪性黒色腫 / 治療 / siRNA
Outline of Final Research Achievements

We synthesized 6 types of CRISPR/Cas9-Mitf corresponding to the Mitf sequence which is involved in melanin synthesis as well as differentiation of melanocyte and melanoma, and transfected them into human melanoma cell lines. CRISPR/Cas9-Mitf-4-mediated knockout of Mitf suppressed viability of HMV-II melanoma by 13% in comparison with control HMV-II melanoma. Low expression of Mitf mRNA in control amelanotic human melanoma SK-Mel 28 and in control melanotic human melanoma G361, and high expression of Mitf mRNA in control melanotic human melanoma HMV-II were confirmed. CRISPR/Cas9-mediated knockout of Mitf might suppress growth of certain types of human melanoma. While Mitf mRNA expression levels in human melanoma might not be associated with melanin production ability.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (40 results)

All 2015 2014 2013 2012

All Journal Article (27 results) (of which Peer Reviewed: 27 results,  Open Access: 9 results) Presentation (12 results) Book (1 results)

  • [Journal Article] Acute generalized exanthematous pustulosis caused by dihydrocodeine phosphate in a psoriasis vulgaris patient with a heterozygous IL36RN mutation2015

    • Author(s)
      Nakai N, Sugiura K, Akiyama M, Katoh N
    • Journal Title

      JAMA Dermatol

      Volume: 151 Issue: 3 Pages: 311-315

    • DOI

      10.1001/jamadermatol.2014.3002

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Severe adult atopic dermatitis successfully treated with concurrent Unseiin and standard treatment2015

    • Author(s)
      Nakai N, Katoh N.
    • Journal Title

      Traditional & Kampo Medicine

      Volume: 2 Issue: 1 Pages: 23-6

    • DOI

      10.1002/tkm2.1011

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Anti-tumor effect of Japanese herbal medicine Ninjinto in mouse melanoma: Efficacy of preventive treatment2015

    • Author(s)
      Nakai N, Kishida T, Katoh N
    • Journal Title

      Traditional & Kampo Medicine

      Volume: 2 Issue: 1 Pages: 14-22

    • DOI

      10.1002/tkm2.1010

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Atypical case of juvenile spring eruption with photosensitivity to a single dose of ultraviolet A irradiation.2014

    • Author(s)
      Ohshita A, Nakai N, Katoh N, Konishi K.
    • Journal Title

      J Dermatol.

      Volume: 41 Issue: 4 Pages: 356-7

    • DOI

      10.1111/1346-8138.12421

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A case of cutaneous squamous cell carcinoma arising in an actinic keratosis on the face in a centenarian woman successfully treated with surgery.2014

    • Author(s)
      Yamazato S, Nakai N, Katoh N.
    • Journal Title

      Indian J Dermatol.

      Volume: 59 Issue: 3 Pages: 304-6

    • DOI

      10.4103/0019-5154.131425

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Nodular primary localized cutaneous amyloidosis in a patient with pulmonary sarcoidosis.2014

    • Author(s)
      Nakai N, Ozawa A, Katoh N.
    • Journal Title

      Indian J Dermatol.

      Volume: 59 Issue: 3 Pages: 307-8

    • DOI

      10.4103/0019-5154.131430

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Maculopapular-Type Drug Eruption Caused by Sitagliptin Phosphate Hydrate: A Case Report and Mini-Review of the Published Work2014

    • Author(s)
      Nakai N, Katoh N.
    • Journal Title

      Allergology International

      Volume: 63 Issue: 3 Pages: 489-491

    • DOI

      10.2332/allergolint.13-LE-0669

    • NAID

      130004687701

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Paraneoplastic dermatomyositis associated with gallbladder carcinoma: a case report and mini-review of the published work.2014

    • Author(s)
      Sawada T, Nakai N, Masuda K, Katoh N.
    • Journal Title

      Indian J Dermatol.

      Volume: 59 Issue: 6 Pages: 615-6

    • DOI

      10.4103/0019-5154.143543

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Multiple fungal infection in a patient on chronic low dose corticotherapy.2014

    • Author(s)
      Yamazato S, Nakai N, Katoh N.
    • Journal Title

      Indian J Dermatol.

      Volume: 59 Issue: 6 Pages: 636-636

    • DOI

      10.4103/0019-5154.143605

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mycosis fungoides palmaris et plantaris successfully treated with radiotherapy: case report and mini-review of the published work.2014

    • Author(s)
      Nakai N, Hagura A, Yamazato S, Katoh N.
    • Journal Title

      J Dermatol.

      Volume: 41 Issue: 1 Pages: 63-7

    • DOI

      10.1111/1346-8138.12308

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Skin tag of the nipple with blister formation: two case reports.2013

    • Author(s)
      Nakai N, Itoh R, Katoh N.
    • Journal Title

      J Dermatol.

      Volume: 40 Issue: 11 Pages: 946-7

    • DOI

      10.1111/1346-8138.12292

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A maculopapular-type eruption associated with deferasirox administration.2013

    • Author(s)
      Ohshita A, Nakai N, Katoh N, Konishi K.
    • Journal Title

      J Am Acad Dermatol.

      Volume: 69 Issue: 5 Pages: e265-e267

    • DOI

      10.1016/j.jaad.2013.07.015

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Maculopapular-Type Drug Eruption Caused by Coughcode<sup> &reg;</sup>-N Combination Tablets2013

    • Author(s)
      Yokose C, Nakai N, Katoh N.
    • Journal Title

      Allergology International

      Volume: 62 Issue: 4 Pages: 519-521

    • DOI

      10.2332/allergolint.13-LE-0558

    • NAID

      130004873084

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Correlation between Soluble Interleukin-2 Receptor Levels and Modified Rodnan Total Skin Thickness Scores in a Patient with Generalized Morphea: A Case Report2013

    • Author(s)
      Nakai N, Hotta E, Asai J, Katoh N.
    • Journal Title

      Allergology International

      Volume: 62 Issue: 3 Pages: 391-393

    • DOI

      10.2332/allergolint.13-LE-0554

    • NAID

      130004477194

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Skin infiltration of nodal peripheral t-cell lymphoma-not otherwise specified identified by skin biopsy of faint eruptions.2013

    • Author(s)
      Isohisa T, Nakai N, Kishimoto M, Katoh N.
    • Journal Title

      Indian J Dermatol.

      Volume: 58 Issue: 3 Pages: 247-247

    • DOI

      10.4103/0019-5154.110899

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A case of sarcoidosis in a patient with systemic sclerosis.2013

    • Author(s)
      Isohisa T, Nakai N, Hanada K, Takenaka H, Katoh N.
    • Journal Title

      Indian J Dermatol.

      Volume: 58 Issue: 2 Pages: 165-165

    • DOI

      10.4103/0019-5154.108119

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Angiokeratoma corporis diffusum in the absence of metabolic disorders: a case report and mini-review of the published work.2013

    • Author(s)
      Yasuike R, Nakai N, Komori S, Katoh N.
    • Journal Title

      J Dermatol.

      Volume: 40 Issue: 8 Pages: 668-9

    • DOI

      10.1111/1346-8138.12187

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Positive Skin Prick Test to Cefcapene Pivoxil Hydrochloride Hydrate: A Case Report2013

    • Author(s)
      Yamazato S, Nakai N, Katoh N.
    • Journal Title

      Allergology International

      Volume: 62 Issue: 2 Pages: 263-264

    • DOI

      10.2332/allergolint.12-LE-0524

    • NAID

      130004873066

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Allergic contact dermatitis due to epsilon-aminocaproic acid: a case report and mini-review of the literature.2013

    • Author(s)
      Yamamoto Y, Wada M, Nakai N, Katoh N.
    • Journal Title

      J Dermatol

      Volume: 40 Issue: 4 Pages: 301-303

    • DOI

      10.1111/1346-8138.12092

    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fixed Drug Eruption Caused by Fluconazole: A Case Report and Mini-Review of the Literature2013

    • Author(s)
      Nakai N, Katoh N.
    • Journal Title

      Allergology International

      Volume: 62 Issue: 1 Pages: 139-141

    • DOI

      10.2332/allergolint.12-LE-0464

    • NAID

      130004477164

    • ISSN
      1323-8930, 1440-1592
    • Related Report
      2013 Research-status Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A case of angiolymphoid hyperplasia with eosinophilia (ALHE) treated successfully with imiquimod.2013

    • Author(s)
      Isohisa T, Masuda K, Nakai A, Katoh N.
    • Journal Title

      Int J Dermatol

      Volume: 印刷中 Issue: 1 Pages: e43-e44

    • DOI

      10.1111/j.1365-4632.2011.05284.x

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] A rare case of basal cell carcinoma arising in a nevus sebaceous on the upper arm.2012

    • Author(s)
      Arakawa Y, Nakai A, Katoh N.
    • Journal Title

      J Dermatol

      Volume: 39 Issue: 11 Pages: 931-932

    • DOI

      10.1111/j.1346-8138.2011.01488.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Immunohistological analysis of in-transit metastasis in a patient with advanced melanoma treated with combination therapy of cytosine guanine dinucleotide oligodeoxynucleotide, dacarbazine and beta-interferon: A case report.2012

    • Author(s)
      Akiko Ozawa, Tomoko Nomiyama, 他5名
    • Journal Title

      The Journal of Dermatology

      Volume: 39 Issue: 12 Pages: 1035-1037

    • DOI

      10.1111/j.1346-8138.2012.01517.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Value of lymphocyte transformation test in the diagnosis of erythema multiforme-type drug eruption due to antiepileptic drug lamotrigine: three cases reports.2012

    • Author(s)
      Nakai N, Katoh N.
    • Journal Title

      J Dermatol

      Volume: 39 Issue: 12 Pages: 1083-1084

    • DOI

      10.1111/j.1346-8138.2012.01601.x

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] A case of advanced cutaneous squamous cell carcinoma of the lower eyelid that was successfully treated with tegafur-gimeracil-oteracil potassium monotherapy.2012

    • Author(s)
      Yamamoto Y, Nakai N, Wakabayashi Y, Okuzawa Y, Asai J, Takenaka H, Katoh N.
    • Journal Title

      J Am Acad Dermatol

      Volume: 67 Issue: 5 Pages: e233-e234

    • DOI

      10.1016/j.jaad.2012.04.038

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Superficial acral fibromyxoma of the great toe: case report and mini-review of the literature.2012

    • Author(s)
      Wakabayashi Y, Nakai N, Takenaka H, Katoh N.
    • Journal Title

      Acta Dermatovenerol Croat.

      Volume: 20 Pages: 263-6

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 著明な血小板減少を伴ったマムシ咬傷の1例2012

    • Author(s)
      奥沢康太郎 中井章淳 加藤則人 末廣晃宏
    • Journal Title

      臨床皮膚科

      Volume: 66 Pages: 737-40

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 両手に多発したReticulohistiocytic Granulomaの1例.2015

    • Author(s)
      上田有希子、中井章淳、加藤則人.
    • Organizer
      第78回日本皮膚科学会東京支部学術大会.
    • Place of Presentation
      東京
    • Year and Date
      2015-02-21 – 2015-02-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] ワンピースに使用された染料による接触皮膚炎の1例.2014

    • Author(s)
      堀田恵理、峠岡理沙、中井章淳、益田浩司、佐々木和実、加藤則人.
    • Organizer
      第44回日本皮膚アレルギー・接触皮膚炎学会総会学術大会.
    • Place of Presentation
      仙台
    • Year and Date
      2014-11-21 – 2014-11-23
    • Related Report
      2014 Annual Research Report
  • [Presentation] 皮膚がん患者に対するMohs ペーストの有用性と適応:自験3例と文献的考察2014

    • Author(s)
      中井章淳,大下彰史,中川有夏,澤田貴裕,田嶋佐妃,奥沢康太郎,加藤則人
    • Organizer
      第137回日本皮膚科学会宮崎地方会
    • Place of Presentation
      宮崎市
    • Related Report
      2013 Research-status Report
  • [Presentation] Mycosis fungoides palmaris et plantaris の1 例.2013

    • Author(s)
      中井章淳,山里志穗,羽倉麻美,加藤則人.
    • Organizer
      第112回日本皮膚科学会総会.
    • Place of Presentation
      横浜市
    • Related Report
      2013 Research-status Report
  • [Presentation] In vitro and in vivo evaluation of the antitumor effect of herbal medicine Ninjin-to in mouse melanoma.2013

    • Author(s)
      Noriaki Nakai, Norito Katoh.
    • Organizer
      9th Asian Dermatological Congress
    • Place of Presentation
      Hong Kong
    • Related Report
      2013 Research-status Report
  • [Presentation] 皮膚クリプトコッカス症の1例2013

    • Author(s)
      山里志穂、横瀬千美、安池理紗、若林祐輔、田嶋佐妃、中井章淳、加藤則人
    • Organizer
      日本皮膚科学会第429回京滋地方会.
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] ラモトリギンによる薬疹―DLSTの有用性―自験3例と本邦報告例の検討.2012

    • Author(s)
      中井章淳、加藤則人.
    • Organizer
      第24回日本アレルギー学会春季臨床大会.
    • Place of Presentation
      大阪
    • Related Report
      2012 Research-status Report
  • [Presentation] Usefulness of lymphocyte transformation test for the analysis of adverse reactions to antiepileptic drug lamotrigine: three cases of erythema multiforme-type drug eruption and review of the literature in Japan.2012

    • Author(s)
      Nakai N, Katoh N.
    • Organizer
      The Second Eastern Asia Dermatology Congress.
    • Place of Presentation
      China
    • Related Report
      2012 Research-status Report
  • [Presentation] 標準治療と温清飲の併用が早期寛解導入に有効と考えられた重症成人型アトピー性皮膚炎の1例.2012

    • Author(s)
      中井章淳.
    • Organizer
      第63回日本東洋医学会学術総会.
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒアレインによる接触皮膚炎の1例.2012

    • Author(s)
      山本祐理子、和田 誠、中井章淳、加藤則人.
    • Organizer
      日本皮膚科学会第425回京滋地方会.
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] 非定型疹を呈した成人Still病の2例.2012

    • Author(s)
      堀田恵理、小森敏史、中井章淳、加藤則人.
    • Organizer
      日本皮膚科学会第427回京滋地方会.
    • Place of Presentation
      滋賀
    • Related Report
      2012 Research-status Report
  • [Presentation] 催涙スプレーによる刺激性接触皮膚炎:呼吸・脈拍に異常をきたした1例.2012

    • Author(s)
      山里志穂、中井章淳、加藤則人.
    • Organizer
      日本皮膚科学会第428回京滋地方会.
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Book] エキスパートが答える!アトピー性皮膚炎Q&A 55.加藤則人(編)2014

    • Author(s)
      中井章淳(分担執筆)
    • Total Pages
      2
    • Publisher
      診断と治療社
    • Related Report
      2014 Annual Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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