| Project/Area Number |
24791178
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Dermatology
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
ITO Tomonobu 東京医科大学, 医学部, 講師 (70398767)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | IL-33 / MHC class II / CD11ｃ / PU.1 / 肥満細胞 / 線維芽細胞（NIH3T3 / サイトカイン・ケモカイン / 転写因子PU.1 |
|---|
| Research Abstract |
We reported that over-expression of PU.1, a myeloid- and lymphoid-specific transcription factor belonging to the Ets family, induces monocyte-specific gene expression in mast cells. In order to identify the factor mediating differentiation into CD11c positive mast cells, we focused our research on fibroblasts co-existing within the dermal structure to determine whether the growth factors secreted by these fibroblasts could induce differentiation into dendritic cell-like cells. Mast cells co-cultured with fibroblasts did fail to express monocyte-specific genes. However, the addition of IL-33 to the co-culture induced MHC class II expression in the mast cells. In conclusion, IL-33 induced MHC class II expression via PU.1.
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