Grant-in-Aid for Young Scientists (B)
Genome-wide DNA methylation profiling (485,764 CpG sites) of peripheral leukocytes was conducted in the first set of samples (24 medication-free patients with SCZ and 23 non-psychiatric controls) using Infinium HumanMethylation450 Beadchips. Second, a monozygotic twin study was performed using three pairs of monozygotic twins that were discordant for SCZ. Finally, the data from these two independent cohorts were compared.A total of 234 differentially methylated CpG sites that were common between these two cohorts were identified. Of the 234 CpG sites, 153 sites (65.4%) were located in the CGIs and in the regions flanking CGIs (CGI: 40.6%; CGI shore: 13.2%; CGI shelf: 11.5%). Of the 95 differently methylated CpG sites in CGIs, most of them were located in the promoter regions (promoter: 75.8%; gene body: 14 .7%; 3'UTR: 2.1%). Aberrant DNA methylation in SCZ was identified at numerous loci across the whole genome in peripheral leukocytes.
Clin Psychopharmacol Neurosci.
Psychiatry Clin Neurosci
J Hum Genet
American Journal of Medical Genetics Part B: Neuropsychiatric Genetics
Attribution of KAKENHI