| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2014: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Outline of Final Research Achievements |
Based on the research implementation plan, I analyzed phosphorylation of dopamine- and cAMP-regulated phosphoprotein of 32 KDa (DARPP-32) which expressed especially in post synaptic cells. I treated mouse striatal slices for cannabinoid receptor agonist stimulation. Experiments were examined by western blotting methods using phospho specific antibody of DARPP-32 threonine 34 residue (Thr 34). The result is that DARPP-32 Thr 34 increase phosphorylation level by cannabinoid receptor agonist expectedly. I completed to generate cell culture for the expression of cannabinoid receptor. However, interaction between glutamate signaling to cannnbinoid is future plan of this work.
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