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Development of a novel system measuring a circadian rhythm of erythrocytes

Research Project

Project/Area Number 24791255
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Psychiatric science
Research InstitutionKeio University

Principal Investigator

OHSAWA Yosuke  慶應義塾大学, 医学部, 助教 (50528429)

Project Period (FY) 2012-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsペルオキシレドキンシン / 概日リズム / 赤血球 / BRET / ペルオキシレドキシン
Research Abstract

I developed a measuring system based on bioluminescence resonance energy transfer (BRET). The system can sequentially monitor the homodimer formation of Peroxyredoxin 2 (PRX2). The temporal resolution of the luminescent data was dramatically improved by measuring the PRX2 dimeric rhythm at 10-minute intervals, which enabled us to calculate the acrophase or free-running period from the luminescent data with high accuracy.
This research suggested that hydrogen peroxide acts as an entraining agent (Zeitgeber) on the oxidation-reduction rhythm of PRX2. The elucidation of the time-keeping mechanism of the redox PRX2 rhythm in erythrocytes which lack a cell nucleus and clock genes may contribute greatly to our understanding of how the humoral or neuronal circadian signals control the peripheral clocks, and how the internal desynchronization between peripheral tissues occurs.

Report

(3 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • Research Products

    (7 results)

All 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (5 results)

  • [Journal Article] In vitro circadian period is associated with circadian/sleep preference.2013

    • Author(s)
      Hida A, 他16名
    • Journal Title

      Scientific Reports

      Volume: 3 Issue: 1 Pages: 2074-2074

    • DOI

      10.1038/srep02074

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rhythmic expression of circadian clock genes in human leukocytes and beard hair follicle cells2012

    • Author(s)
      Watanabe M.
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 425(4) Issue: 4 Pages: 902-7

    • DOI

      10.1016/j.bbrc.2012.08.008

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] 概日リズム睡眠障害患者の体内時計機能障害2013

    • Author(s)
      肥田昌子, 北村真吾, 大澤要介, 中崎恭子, 元村裕貴, 綾部直子, 片寄泰子, 野崎健太郎, 榎本みのり, 加藤美恵, 小野浩子, 亀井雄一, 三島和夫
    • Organizer
      第128回日本薬理学会関東部会
    • Place of Presentation
      東京(新宿区)
    • Year and Date
      2013-07-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 概日リズム睡眠障害患者の体内時計機能障害2013

    • Author(s)
      肥田昌子、北村真吾、大澤要介、中崎恭子、元村裕貴、綾部直子、片寄泰子、野崎健太郎、榎本みのり、加藤美恵、小野浩子、亀井雄一、三島和夫
    • Organizer
      日本薬理学会関東部会
    • Place of Presentation
      早稲田大学国際会議場
    • Related Report
      2013 Annual Research Report
  • [Presentation] 末梢時計機能は個人の概日/睡眠特性を反映する2012

    • Author(s)
      肥田昌子, 北村真吾, 片寄泰子, 榎本みのり, 大澤要介, 渡邊真紀子, 野崎健太郎, 有竹清夏, 樋口重和, 加藤美恵, 守口善也, 亀井雄一, 池田政明, 三島和夫
    • Organizer
      Neuroscience2012
    • Place of Presentation
      愛知(名古屋市)
    • Related Report
      2013 Final Research Report
  • [Presentation] 末梢時計リズムは個人の睡眠特性・生物時計機能を反映する2012

    • Author(s)
      肥田昌子, 北村真吾, 大澤要介, 片寄泰子, 野崎健太郎, 榎本みのり, 有竹清夏, 樋口重和, 加藤美恵, 亀井雄一, 池田政明, 三島和夫
    • Organizer
      第19回日本時間生物学会学術大会
    • Place of Presentation
      北海道(札幌市)
    • Related Report
      2013 Final Research Report
  • [Presentation] 生体組織を利用したヒト生物時計機能評価-概日リズム睡眠障害患者への応用-2012

    • Author(s)
      肥田昌子, 北村真吾, 大澤要介, 榎本みのり, 片寄泰子, 野崎健太郎, 加藤美恵, 守口善也, 亀井雄一, 三島和夫
    • Organizer
      日本睡眠学会第37回定期学術集会
    • Place of Presentation
      神奈川(横浜市)
    • Related Report
      2013 Final Research Report

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Published: 2013-05-31   Modified: 2019-07-29  

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