Study for the realization of tailor maid high precision radiotherapy for breast cancer based on molecular biology

• Project/Area Number: 24791259
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Hokkaido University
• Principal Investigator: Kinoshita Rumiko 北海道大学, 大学病院, 助教 (70507582)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000); Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 乳房温存療法 / GEP100 / 局所再発 / 陽子線治療 / 領域リンパ節照射 / AMAP1 / Afr6 / GEP10 / 乳癌 / 術後照射 / 分子生物学 / 放射線腫瘍学

Outline of Final Research Achievements

Two studies about breast conservation therapy (BCT) were performed.First study was performed to investigate whether the presence of the receptor tyrosine kinase-GEP100-Arf6-AMAP1 pathway correlate with local recurrence (LR) after BCT. Using 19 specimens with LR, immunohistochemical staining of EGFR, HER2, GEP100 and AMAP1 was performed. Strongly positive for both GEP100 and AMAP1 group shows a shorter time to LR after the completion of radiotherapy than the others.This study shows that co-overexpression of GEP100 and AMAP1 protein correlate with rapid LR after BCT. Next, dosimetric comparison study which compare spot scanning proton therapy (SSPT) to intensity modulated radiotherapy (IMRT). Three different planning target volume (PTV) which include affected breast with or without regional lymph node. Dose to PTVs and organ at risks (OARs; ipsilateral lung, heart and contralateral lung) were calculated and compared. The SSPT reduced the dose to OARs with adequate target coverage.

Research Products

• [Journal Article] Co-overexpression of GEP100 and AMAP1 Protein Correlates with Rapid Local Recurrence after Breast Conservative Therapy (2013)
  • Author(s): Kinoshita R, Nam JM, Ito YM, Hatanaka KC, Hashimoto A, Hanada H, Osuka Y, Hashimoto S, Onodera Y, Hosoda M, Onodera S, Shimizu S, Tanaka S, Shirato H, Tanino M, Sabe H,
  • Journal Title: PLoS One Volume: 8
  • NAID: 120005348196
  • Peer Reviewed
• [Presentation] Ａｎａｌｙｓｉｓ ｏｆ ｖｏｌｕｍｅｔｒｉｃ ｄｏｓｅ ｏｆ ｔｈｅ ｌｕｎｇ ａｎｄ ｔｈｅ ｈｅａｒｔ ｆｏｒ ｔａｎｇｅｎｔｉａｌ ｂｒｅａｓｔ ｉｒｒａｄｉａｔｉｏｎ (2016)
  • Author(s): Ｒｕｍｉｋｏ Ｋｉｎｏｓｈｉｔａ，Ｙｕｋｉｋｏ Ｎｉｓｈｉｋａｗａ， Ｋｅｎｔａｒｏ Ｎｉｓｈｉｏｋａ， Ｔａｋａｙｕｋｉ Ｈａｓｈｉｍｏｔｏ， Ｓｈｉｎｉｃｈｉ Ｓｈｉｍｉｚｕ， Ｒｙｕｓｕｋｅ Ｓｕｚｕｋｉ， Ｈｉｒｏｋｉ Ｓｈｉｒａｔｏ
  • Organizer: Ｔｈｅ ７５ｔｈ Ａｎｎｕａｌ ｍｅｅｔｉｎｇ ｏｆ ｔｈｅ Ｊａｐａｎ Ｒａｄｉｏｌｏｇｉｃａｌ Ｓｏｃｉｅｔｙ
  • Place of Presentation: Pacifico Yokohama (Ｙｏｋｏｈａｍａ，Ｋａｎａｇａｗａ)
  • Year and Date: 2016-04-14
• [Presentation] Ｉｓ ｓｐｏｔ ｓｃａｎｎｉｎｇ ｐｒｏｔｏｎ ｔｈｅｒａｐｙ ｆｏｌｌｏｗｉｎｇ ｂｒｅａｓｔ ｃｎｓｅｒｖａｔｉｏｎ ｓｕｒｇｅｒｙ ｂｅｎｅｆｉｃｉａｌ？ (2015)
  • Author(s): Ｒｕｍｉｋｏ Kinoshita, Takaaki Yoshimura, Shinishi Shimizu, Yukiko Nishikawa, Kentrao Nishioka,Ｔａｋａｙｕｋｉ Ｈａｓｈｉｍｏｔｏ， Ｔａｅｋｏ Ｍａｔｓｕｕｒａ， Ｓｅｉｓｈｉｎ Ｔａｋａｏ， Ｋｉｋｕｏ Ｕｍｅｇａｋｉ， Ｈｉｒｏｋｉ Ｓｈｉｒａｔｏ
  • Organizer: Ｔｈｅ 28th ａｎｎｕａｌ ｍｅｅｔｉｎｇ ｏｆ ｔｈｅ Ｊａｐａｎｅｓｅ Ｓｏｃｉｅｔｙ ｆｏr Ｒａｄｉａｔｉｏｎ Ｏｎｃｏｌｏｇｙ
  • Place of Presentation: Beisia Culture Hall (Ｍａｅｂａｓｈｉ，Ｇｕｎｍａ)
  • Year and Date: 2015-11-19
• [Presentation] Co-overexpression of GEP100 and AMAP1 Protein Correlates with Rapid Local Recurrence after Breast Conservative Therapy (2014)
  • Author(s): Rumiko Kinoshita
  • Organizer: 1st GI-CORE Medical Science and Engineering Symposium
  • Place of Presentation: Sapporo Park Hotel (Sapporo)
  • Invited
• [Presentation] Co-overexpression Of GEP100 With EGFR Correlates With Early Recurrence After Breast Conservation Therapy (BCT) (2012)
  • Author(s): Kinoshita R
  • Organizer: The 54 th annual meeting of the American Society of Therapeutic Radiology and Oncology
  • Place of Presentation: Boston Convention and Exhibition Center, Boston, USA