Differentiated embryonic chondrocyte expressed gene regulates carcinogenesis and apoptotic pathway of squamous cell carinoma.

• Project/Area Number: 24791265
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Radiation science
• Research Institution: Hirosaki University
• Principal Investigator: SEINO Hiroko 弘前大学, 医学(系)研究科(研究院), 助手 (30598727)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
• Keywords: 時計遺伝子 / DEC / 扁平上皮癌 / 食道癌 / 治療 / 癌 / 概日リズム / アポトーシス

Outline of Final Research Achievements

Differentiated embryonic chondrocyte expressed gene 1 (DEC1; BHLHE40/Stra13/Sharp2) and differentiated embryonic chondrocyte expressed gene 2 (DEC2; BHLHE41/Sharp1) are basic helix-loop-helix (bHLH) transcriptional factors that are involved in the regulation of cell differentiation, circadian rhythms, response to hypoxia and carcinogenesis. DEC1 is overexpressed in various tumor regions. High levels of DEC1 mRNA are also detected in an array of cancer cell lines. We reported that DEC1 has pro-apoptotic effects, whereas DEC2 has no apoptotic effect on human esophageal cancer TE10 cells. DEC2 has anti-apoptotic effects, whereas DEC1 has no apoptotic effect on human oral cancer HSC-3 cells. DEC1 and DEC2 have no apoptotic effect in TE5 and CA9-22. These different apoptotic effects of DEC1 and DEC2 may depend on the differences of cell lines and/or gene expression. However, the mechanism of DEC in tumor apoptotic pathway is poorly understood, so further studies are needed.

Research Products

• [Journal Article] <b>Basic helix-loop-helix transcription factor DEC1 regulates the cisplatin-induced apoptotic pathway of human esophageal cancer </b><b>cells </b> (2015)
  • Author(s): Seino H
  • Journal Title: Biomedical Research Volume: 36 Issue: 2 Pages: 89-96
  • DOI: 10.2220/biomedres.36.89
  • NAID: 130005065284
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Involvement of c-Myc in the proliferation of MCF-7 human breast cancer cells induced by bHLH transcription factor DEC2. (2015)
  • Author(s): Wu Y
  • Journal Title: Int J Mol Med. Volume: 35 Issue: 3 Pages: 815-20
  • DOI: 10.3892/ijmm.2014.2042
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Molecular pathology and carcinogenesis of biliary cancer (2015)
  • Author(s): Kijima H
  • Journal Title: Nihon Rinsho. Volume: 73 Pages: 449-53
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Incidental prostate 18F-FDG uptake without calcification indicates the possibility of prostate cancer. (2014)
  • Author(s): Seino H, Ono S, Miura H, Morohashi S, Wu Y, Tsushima F, Takai Y, Kijima H.
  • Journal Title: Oncol Rep. Volume: 31 Issue: 4 Pages: 1517-1522
  • DOI: 10.3892/or.2014.3011
  • Peer Reviewed / Open Access
• [Journal Article] Pathological characteristics of early to advanced gallbladder carcinoma and extrahepatic cholangiocarcinoma. (2014)
  • Author(s): Kijima H, Wu Y, Yosizawa T, Suzuki T, Tsugeno Y, Haga T, Seino H, Morohashi S, Hakamada K.
  • Journal Title: J Hepatobiliary Pancreat Sci. Volume: 21 Issue: 7 Pages: 453-8
  • DOI: 10.1002/jhbp.72
  • Peer Reviewed / Open Access
• [Journal Article] The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer (2012)
  • Author(s): Wu, Y., Kawamoto, T., Noshiro, M., et al.(他10名)
  • Journal Title: Int J Oncol Volume: 41 Issue: 4 Pages: 1337-1346
  • DOI: 10.3892/ijo.2012.1559
  • Peer Reviewed
• [Journal Article] BHLH transcription factor DEC2 regulates pro-apoptotic factor Bim in human oral cancer HSC-3 cells. (2012)
  • Author(s): Wu Y, Sato F, Bhawal UK, Kawamoto T, Fujimoto K, Noshiro M, Seino H, Morohashi S, Kato Y, Kijima H.
  • Journal Title: Biomed Res. Volume: 33 Pages: 75-82
  • NAID: 130004470811
  • Peer Reviewed
• [Journal Article] Smad3 and Snail show circadian expression in human gingival fibroblasts, human mesenchymal stem cell, and in mouse liver. (2012)
  • Author(s): Sato F, Sato H, Jin D, Bhawal UK, Wu Y, Noshiro M, Kawamoto T, Fujimoto K, Seino H, Morohashi S, Kato Y, Kijima H.
  • Journal Title: BiochemBiophys Res Commun. Volume: 419 Pages: 441-446
  • Peer Reviewed
• [Presentation] BHLH transcription factor DEC2 inhibits TGF-β-induced tumor progression in BxPC-3 cells (2013)
  • Author(s): Yunyan Wu, Hiroko Seino, Takahiro Suzuki, Satoko Morohashi, Tadashi Yoshizawa, Yukio Kato, Hiroshi Kijima.
  • Organizer: 第102回病理学会総会
  • Place of Presentation: 北海道札幌市
• [Presentation] Basic helix-loop-helix transcription factor DEC2 negatively regulates pro-apoptotic factor Bim in cisplatin-treated human oral cancer cells (2012)
  • Author(s): Yunyan Wu, Hiroko Seino, Satoko Morohashi, Tadashi Yoshizawa, Yukio Kato, Hiroshi Kijima.
  • Organizer: 第9回日本病理学会カンファレンス
  • Place of Presentation: 山口県山口市
• [Presentation] PERIOD is involved in the proliferation of human pancreatic MIA-PaCa2 cancer cells. (2012)
  • Author(s): Takahiro Suzuki, Yunyan Wu, Hiroko Seino, Satoko Morohashi, Toshihiro Haga, Yukio Kato, Hiroshi Kijima.
  • Organizer: 第9回日本病理学会カンファレンス
  • Place of Presentation: 山口県山口市