| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Tenascin-C (TN-C) is expressed transiently inflammatory regions in various cardiovascular diseases. We investigated the possibility that specific antibody against TN-C is useful for detecting cardiovascular diseases as a molecular diagnostic reagent. Heavy chain variable region (VH) and light chain variable region (VL) genes of anti-TN-C antibody were obtained from total RNA in hybridoma, and were performed overlap-extended PCR. Expression vector inserted VH-linker-VL gene (scFv) was used in E. coli, in vitro cell free (RTS wheat germ) and mammalian cell line (HEK293) expression systems. As a result, the antibody expression was highly effective in HEK293 cell line system. As soon as it’s ready, we are planning to experiments of antibody accumulation in foci of myocarditis model mice.
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