Establishment of diagnosis based on molecular targeting imaging of cardiovascular diseases by scFv antibody against Tenascin-C
| Project/Area Number |
24791291
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Radiation science
|
|---|
| Research Institution | Mie University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
|---|
| Keywords | ScFv型抗体 / テネイシン-C / 分子イメージング / 虚血性心疾患 / ScFv / 心筋炎 / イメージング / 分子標的 / SPECT / アイソトープ標識 |
|---|
| Outline of Final Research Achievements |
Tenascin-C (TN-C) is expressed transiently inflammatory regions in various cardiovascular diseases. We investigated the possibility that specific antibody against TN-C is useful for detecting cardiovascular diseases as a molecular diagnostic reagent. Heavy chain variable region (VH) and light chain variable region (VL) genes of anti-TN-C antibody were obtained from total RNA in hybridoma, and were performed overlap-extended PCR. Expression vector inserted VH-linker-VL gene (scFv) was used in E. coli, in vitro cell free (RTS wheat germ) and mammalian cell line (HEK293) expression systems. As a result, the antibody expression was highly effective in HEK293 cell line system. As soon as it’s ready, we are planning to experiments of antibody accumulation in foci of myocarditis model mice.
|
Report
(4 results)
Research Products
(27 results)
-
-
-
-
-
-
-
-
[Presentation] A Functional Role of Tenascin-C in Mouse hypertensive heart.2014
Author(s)
Shimojo, N., Hashizume, R., Kanayama, K., Suzuki, Y., Hara, M., Nishioka, T., Yoshida, T., Imanaka-Yoshida, K.
Organizer
American Heart Association Scientific Sessions 2014
Place of Presentation
シカゴ、アメリカ
Year and Date
2014-11-15 – 2014-11-19
Related Report
-
-
-
[Presentation] KNOCKOUT OF TENASCIN-C REDUCES CARDIOVASCULAR FIBROSIS BY ACTIVATION OF MACROPHAGES VIA INTEGRIN αVβ3/NF-κB IN MOUSE HYPERTENSIVE HEART.2014
Author(s)
Shimojo, N., Hashizume, R., Kanayama, K., Suzuki, Y., Hara, M., Nishioka, T., Yoshida, T., Imanaka-Yoshida, K.
Organizer
The 16th International SHR Symposium
Place of Presentation
イタリア、ローマ
Year and Date
2014-06-18
Related Report
-
[Presentation] Tenascin-C may accelerate cardiac fibrosis by activating macrophages via integrin αVβ3 /NFκb/IL-6 axis2014
Author(s)
Shimojo, N., Hashizume, R., Kanayama, K., Suzuki, Y., Hara, M., Nishioka, T., Yoshida, T., Imanaka-Yoshida, K.
Organizer
The European and the International Societies of Hypertension 2014 Athens, Greece
Place of Presentation
ギリシャ、アテネ
Year and Date
2014-06-13 – 2014-06-16
Related Report
-
[Presentation] Tenascin-C accelerates cardiac fibrosis by activating macrophages via integrin αVβ3/NFκb/IL-6 axis.2014
Author(s)
Shimojo, N., Hashizume, R., Kanayama, K., Suzuki, Y., Hara, M., Nishioka, T., Yoshida, T., Imanaka-Yoshida, K.
Organizer
22nd International Symposium on Molecular Cell Biology of MACROPHAGES 2014
Place of Presentation
神戸商工会議所
Year and Date
2014-06-02 – 2014-06-03
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
