Radiosensitivity test during low dose rate irradiation in normal and repair deficient cells
| Project/Area Number |
24791338
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | Keio University |
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Principal Investigator |
TANAKA Tomoki 慶應義塾大学, 医学部, 助教 (80594598)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 低線量被曝 / 放射線感受性 / 染色体解析 / 低線量率 / 低線量照射 |
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| Research Abstract |
We exposed normal and ataxia telangiectasia cells to 0.5 Gy per day to gamma ray. Cells were maintained under Go during irradiation. The dose was 1 to 3 Gy and chromosomal aberrations using fusion premature chromosomal condensation technique were analyzed. Giemsa staining showed that 1 Gy induces around 0.36 fragments in normal cells and around 1.35 in AT cells, indicating that AT cells rejoin breaks less effectively than normal cells. We also analyzed chromosomal exchange after exposure to 3 Gy using FISH techniques. AT cells have more misrejoined breaks. When cells irradiated with 3 Gy were subcultured and G2 chromosomal aberrations were analyzed using calyculin-A induced PCC technique, unrejoined breaks decreased and misrejoined breaks increased in both cell lines, suggesting that AT cells begin to rejoin breaks when a certain number of beaks are accumulated and an increased number of exchanges were observed in G0 AT cells, which is similar situation after high-dose-rate irradiation
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Report
(3 results)
Research Products
(4 results)
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[Presentation] 前立腺癌に対するIMRT の初期経験2013
Author(s)
田中智樹, 栗林 徹, 池本孝司, 三嶽秀介, 小切孝洋, 金田朋也, 小池直義, 花田剛士, 茂松直之
Organizer
第443回 日本医学放射線学会 関東地方会
Place of Presentation
東京
Year and Date
2013-06-01
Related Report
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