Bystander response in chondrosarcoma cells
| Project/Area Number |
24791354
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Radiation science
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| Research Institution | National Institute of Radiological Sciences |
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Principal Investigator |
WAKATSUKI Masaru 独立行政法人放射線医学総合研究所, 重粒子医科学センター, 医長 (40399449)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | バイスタンダー効果 / 炭素イオン線 / 重粒子線 / 軟骨肉腫 / LET / 国際情報交換 |
|---|
| Research Abstract |
The bystander effect, whereby cells that are not traversed by ionizing radiation exhibit various responses when in proximity to irradiated cells, is well documented in the field of radiation biology. In the present work, we investigated the bystander response induced by ionizing radiation in chondrosarcoma cells.
Human chondrosarcoma cells (HTB94) and human skin fibroblasts (AGO1522) were irradiated with carbon irradiation of varied Linear energy transfer (LET) using a transwell insert co-culture system that precludes physical contact between targeted and bystander cells. We investigated the bystander signaling between mixed cultures of tumor cells and normal human skin fibroblasts (AGO1522). We analyzed DNA damage by using micronucleus formation assays.
Bystander AG01522 fibroblasts show increases above the levels of MN in control cells after co-culture with tumor cells. However, there were no differences between high and low LET.
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Report
(3 results)
Research Products
(22 results)
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[Journal Article] Clinical outcomes of carbon ion radiotherapy for locally advanced adenocarcinoma of the uterine cervix in phase I / II clinical trial (9704)2014
Author(s)
Masaru Wakatsuki, Shingo Kato, Tatsuya Ohno, Kumiko Karasawa, Tomoaki Tamaki, Ken Ando, Hiroki Kiyohara, Hirohiko Tsujii, Takashi Nakano, Tadashi Kamada, Makio Shozu,
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Journal Title
Cancer
Volume: Epub ahead of print
Issue: 11
Pages: 1663-1669
DOI
Related Report
Peer Reviewed
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[Journal Article] Dose-escalation study of carbon ion radiotherapy for locally advanced squamous cell carcinoma of the uterine cervix (9902).2014
Author(s)
Masaru Wakatsuki, Shingo Kato, Tatsuya Ohno, Kumiko Karasawa, Ken Ando, Hiroki Kiyohara, Hirohiko Tsujii, Takashi Nakano, Tadashi Kamada, Makio Shozu,
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Journal Title
Gynecologic oncology
Volume: 132
Issue: 1
Pages: 87-92
DOI
Related Report
Peer Reviewed
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[Journal Article] Health-related quality of life after carbon-ion radiotherapy for prostate cancer: A 3-year prospective study.2014
Author(s)
Katoh H, Tsuji H, Ishikawa H, Kamada T, Wakatsuki M, Hirasawa N, Suzuki H, Akakura K, Nakano T, Shimazaki J, Tsujii H.
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Journal Title
Int J Urol.
Volume: 21
Issue: 4
Pages: 370-5
DOI
Related Report
Peer Reviewed
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[Presentation] Carbon ion Radiotherapy for locally advanced adenocarcinoma of the uterinecervix2013
Author(s)
Masaru Wakatuki, Shingo Kato, Tatsuya Ohno, Kumiko Karasawa, Ken Ando, Hiroki Kiyohara, Daisuke Irie, Takashi Nakano, Tadashi Kamada, Makio Syozu
Organizer
HITSRS2013
Place of Presentation
The Keiyo Bank Culture Plaza, Chiba
Related Report
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