The identification of HLA class II DR associated molecules contributing to Accommodation

• Project/Area Number: 24791371
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Nagoya University
• Principal Investigator: IWASAKI Kenta 名古屋大学, 医学(系)研究科(研究院), 寄附講座講師 (10508881)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 免疫順応 / シグナル伝達 / 抗体関連型拒絶反応 / HLA抗体関連型拒絶反応 / 移植 / HLA

Research Abstract

Expression of HLA-DR on endothelial cells was elicited by IFNg treatment. HLA class I expression was also increased about 4-5 fold. When treated with IFNg, anti-HLA class I and DR antibody binding to endothelial cells did not show any cytoprotective effect against complement-mediated cytotoxicity (CDC) and activated AKT signal, while pre-incubation with anti-A antibody habe resistance to CDC. Anti-HLA class II DR IgG antibody purified from patient sera decreased CD59 expression.

Research Products

• [Journal Article] Influence of olive-derived hydroxytyrosol on the toll-like receptor 4-dependent inflammatory response of mouse peritoneal macrophages (2014)
  • Author(s): Takeda Y, Bui VN, Iwasaki K, Kobayashi T, Ogawa H, Imai K
  • Journal Title: Biochem Biophys Res Commun Volume: 18;446(4) Pages: 1225-30
• [Journal Article] Comparative analysis of drug action on B-cell proliferation and differentiation for mycophenolic acid, everolimus, and prednisolone (2014)
  • Author(s): Haneda M, Owaki M, Kuzuya T, Iwasaki K, Miwa Y, Kobayashi T
  • Journal Title: Transplantation Volume: 27;97(4) Pages: 405-12
• [Journal Article] Comparative analysis of drug action on B-cell proliferation and differentiation for mycophenolic acid, everolimus, and prednisolone. (2014)
  • Author(s): Haneda M, Owaki M, Kuzuya T, Iwasaki K, Miwa Y, Kobayashi T.
  • Journal Title: Transplantation Volume: 97(4) Pages: 405-12
  • Peer Reviewed
• [Journal Article] AMP-activated protein kinase as a promoting factor, but complement and thrombin as limiting factors for acquisition of cytoprotection : implications for induction of accommodation (2013)
  • Author(s): Iwasaki K, et al
  • Journal Title: Transplant International Volume: 26(11) Pages: 1138-48
• [Journal Article] Role of AMP-Activated Protein Kinase in Ferritin H Gene Expression by Resveratrol in Human T Cells (2013)
  • Author(s): Iwasaki K, et al
  • Journal Title: Biochemistry Volume: 30;52(30) Pages: 5075-83
• [Journal Article] Transcriptional regulation of the human ferritin gene by coordinated regulation of Nrf2 and protein arginine methyltransferase PRMT1 andPRMT4 (2013)
  • Author(s): Huang BW, Ray PD, Iwasaki K, , Tsuji Y
  • Journal Title: FASEB J Volume: 27(9) Pages: 3763-74
• [Journal Article] Phosphorylation of 4E-BP1 predicts sensitivity to everolimus in gastric cancer cells (2013)
  • Author(s): Nishi T, Iwasaki K, , et al
  • Journal Title: Cancer Lett Volume: 1;331(2) Pages: 220-9
• [Journal Article] Phosphorylation of 4E-BP1 predicts sensitivity to everolimus in gastric cancer cells. (2013)
  • Author(s): Nishi T, Iwasaki K, Ohashi N, Tanaka C, Kobayashi D, Nakayama G, Koike M, Fujiwara M, Kobayashi T, Kodera Y.
  • Journal Title: Cancer Lett. Volume: 331(2) Pages: 220-9
  • Peer Reviewed
• [Journal Article] Transcriptional regulation of the human ferritin gene by coordinated regulation of Nrf2 and protein arginine methyltransferases PRMT1 and PRMT4. (2013)
  • Author(s): Huang BW, Ray PD, Iwasaki K, Tsuji Y.
  • Journal Title: FASEB J. Volume: 27(9) Pages: 3763-74
  • Peer Reviewed
• [Journal Article] Role of AMP-activated protein kinase in ferritin H gene expression by resveratrol in human T cells. (2013)
  • Author(s): Iwasaki K, Ray PD, Huang BW, Sakamoto K, Kobayashi T, Tsuji Y.
  • Journal Title: Biochemistry Volume: 52(30) Pages: 5075-83
  • Peer Reviewed
• [Presentation] Impliaction of complement regulatory proteins and AMP-regulated kinase for graft accommodation (2013)
  • Author(s): Kenta IWASAKI, Jing PING, Yuko MIWA, Masataka HANEDA, Akira ONISHI, Haruko OGAWA, Fumiaki NAKASHIMA, Takaaki KOBAYASHI
  • Organizer: 第12回国際異種移植学会
  • Place of Presentation: 大阪・大阪国際会議場
  • Year and Date: 2013-11-10
• [Presentation] 抗HLA-DR 抗体は補体制御因子の発現を減少させ, 補体障害を増強させる (2013)
  • Author(s): 岩崎研太, 荊萍, 中島文明, 三輪祐子, 羽根田正隆, 小林孝彰
  • Organizer: 第21回組織適合性学会
  • Place of Presentation: 福島・コラッセ福島
  • Year and Date: 2013-09-14
• [Presentation] シグナル伝達と移植 : 抗体関連型拒絶反応の克服をめざして (2013)
  • Author(s): 岩崎研太, 稲永由紀子, 中島文明, 三輪祐子, 羽根田正隆, 渡井至彦, 片山昭男, 打田和治, 小林孝彰
  • Organizer: 第49回移植学会
  • Place of Presentation: 京都・京都国際会議場
  • Year and Date: 2013-09-05
• [Presentation] Analysis of miRNAs in Peripheral Blood Mononuclear Cells : Potential value of miR-182 as a biomarker of chronic antibody mediated rejection (2013)
  • Author(s): Kenta IWASAKI, Jing PING, Takayuki YAMAMOTO, Yuko MIWA, Masataka HANEDA, Yoshihiko WATARAI, Akio KATAYAMA, Kazuharu UCHIDA and Takaaki KOBAYASHI
  • Organizer: 2013 アメリカ移植学会
  • Place of Presentation: シアトル・シアトルコンベンショナルセンター
  • Year and Date: 2013-05-20
• [Presentation] AMPK Activator Promotes, but Complement and Thrombin Inhibit Anti-A/B Antibody-Induced Accommodation in Endothelial Cells. (2013)
  • Author(s): Kenta Iwasaki, Yuko Miwa, Masataka Haneda, Takafumi Kuzuya, Haruko Ogawa, Akira Onishi, Takaaki Kobayashi.
  • Organizer: アメリカ移植学会
  • Place of Presentation: Seattle, USA
• [Presentation] HLA-DR抗体接着はCD59の発現を減少させる。 (2013)
  • Author(s): 岩﨑研太、荊萍、中島文明、三輪祐子、羽根田正隆、小林孝彰
  • Organizer: 移植学会
  • Place of Presentation: 国立京都国際会館（京都市）
• [Presentation] Implication of complement regulatory proteins and AMP-regulated kinase for graft accommodation (2013)
  • Author(s): Kenta Iwasaki, Jing Ping, Yuko Miwa, Masataka Haneda, Akira Onishi, Haruko Ogawa, Fumiaki Nakashima, Takaaki Kobayashi
  • Organizer: 国際異種移植学会
  • Place of Presentation: 大阪国際会議場（大阪市）
• [Presentation] Accommodation（免疫順応）に関わるシグナル伝達経路の解明 (2012)
  • Author(s): 岩崎研太
  • Organizer: 移植学会
  • Place of Presentation: ウインク愛知（名古屋）