Immunological monitoring after lung transplantation by measurement of serum human leukocyte antigen specific IgM

Research Project

Project/Area Number	24791377
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	General surgery
Research Institution	Okayama University
Principal Investigator	MIYOSHI Kentaroh 岡山大学, 大学病院, 助教 (50534773)
Project Period (FY)	2012-04-01 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2014: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywords	急性拒絶反応 / 肺移植 / 液性免疫 / 細胞性免疫 / モニタリング / 移植免疫 / 抗HLA抗体価 / IgM / 抗HLA抗体 / 免疫学的活動性
Outline of Final Research Achievements	This study aimed to examine that HLA-specific IgM in lung transplant recipient’s sera can be a reasonable marker for immunological activity of acute rejection. As a methodology for HLA-IgM detection, the Flow PRA method was employed and reasonable detectability was confirmed. HLA-IgM value at the time of development of acute rejection was significantly higher than that of immunologically stable condition. Furthermore, the serum HLA-IgM value was unaffected by nonspecific inflammatory response caused by acute stress of common thoracic surgery. This study indicates that monitoring HLA-IgM can contribute to predicting the development of acute lung transplant rejection and determining optimal immunosuppressive management.