Novel diagnostic and therapeutic method using genomic instability of breast cancer.

• Project/Area Number: 24791382
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: National Hospital Organization, Kyushu Cancer Center (2013); Nagasaki University (2012)
• Principal Investigator: OIKAWA Masahiro 独立行政法人国立病院機構(九州がんセンター臨床研究センター), その他部局等, 乳腺科フェロー (90612416)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ゲノム不安定性 / 乳癌 / アレイCGH / 染色体構造変化 / 腫瘍内不均一性 / breast cancer / genome instability / array CGH / FFPE / 乳腺腫瘍 / 診断

Research Abstract

High risk patients among ER-positive/HER2-negative breast cancer can be stratified by intra-tumoral heterogeneity as a surrogate marker for genomic instability. The double-stranded DNA ratio of tumor DNA predicted the performance of DNA from FFPE samples on the aCGH analysis. Quality control by this metrics can utilize valuable FFPE archival tissue on aCGH analysis. our novel diagnostic method, which targets genomic instability, can clearly distinguish cancers from benign tumors of breast intracystic lesions with minimum invasion, thereby avoiding the need for surgical excisional biopsy. Novel therapeutic strategy targeting genomic instability in breast cancer, which is dual administration of Olaparib and Gimeracil, showed growth inhibition of breast cancer cell line with high genomic instability.

Research Products

• [Journal Article] A novel diagnostic method targeting genomic instability in intracystic tumors of the breast (2014)
  • Author(s): Oikawa M, Yano H, Matsumoto M, Otsubo R, Shibata K, Hayashi T, Abe K, Kinoshita N, Yoshiura KI, Nagayasu T
  • Journal Title: Breast Cancer Volume: (Epub ahead of print)
  • NAID: 120006986911
  • Peer Reviewed
• [Journal Article] ホルモン療法における新しい展開 (2014)
  • Author(s): 及川将弘, 大野真司
  • Journal Title: Pharma Medica Volume: 32(5) Pages: 7-11
• [Journal Article] Novel diagnostic procedure for determining metastasis to sentinel lymph nodes in breast cancer using a semi-dry dot-blot (SDB) method (2014)
  • Author(s): Otsubo R, Oikawa M, Hirakawa H, Shibata K, Abe K, Hayashi T, Kinoshita N, Shigematsu K, Hatachi T, Yano H, Matsumoto M, Takagi K, Tsuchiya T, Tomoshige K, Nakashima M, Taniguchi H, Omagari T, Itoyanagi N, Nagayasu T
  • Journal Title: IJC Volume: 134(4) Pages: 905-912
  • Peer Reviewed
• [Journal Article] A novel diagnostic method targeting genomic instability in intracystic tumors of the breast. (2014)
  • Author(s): Oikawa M, Yano H, Matsumoto M, Otsubo R, Shibata K, Hayashi T, Abe K, Kinoshita N, Yoshiura KI, Nagayasu T.
  • Journal Title: Breast Cancer Volume: Jan Issue: 5 Pages: 529-535
  • DOI: 10.1007/s12282-013-0516-9
  • NAID: 120006986911
  • Peer Reviewed
• [Journal Article] Acase of immediate reconstruction using autologous free dermal fat graft after breast conservative surgery (2013)
  • Author(s): Matsumot A, Yano H, Matsumoto M, Oikawa M, Kinoshita N, Abe K, Satoh N, Anami M, Hayashi T, Nagayasu T
  • Journal Title: Nagasaki Medical Journal Volume: 88(2) Pages: 153-157
  • Peer Reviewed
• [Journal Article] A predictive factor of microarray comparative genomic hybridization analysis quality for formalin-fixed paraffin-embedded archival tissue (2013)
  • Author(s): Nakao K, Oikawa M, Arai J, Mussazhanova Z, Kondo H, Shichijo K, Nakashima M, Hayashi T, Yoshiura K, Hatachi T, Nagayasu T
  • Journal Title: Diagn Mol Pathol Volume: 22(3) Pages: 174-180
  • Peer Reviewed
• [Journal Article] Novel diagnostic procedure for determining metastasis to sentinel lymph nodes in breast cancer using a semi-dry dot-blot method. (2013)
  • Author(s): Otsubo R, Oikawa M, Hirakawa H, Shibata K, Abe K, Hayashi T, Kinoshita N, Shigematsu K, Hatachi T, Yano H, Matsumoto M, Takagi K, Tsuchiya T, Tomoshige K, Nakashima M, Taniguchi H, Omagari T, Itoyanagi N, Nagayasu T
  • Journal Title: International Journal of Cancer Volume: 134(4) Issue: 4 Pages: 905-912
  • DOI: 10.1002/ijc.28408
  • Peer Reviewed
• [Journal Article] A predictive factor of the quality of microarray comparative genomic hybridization analysis for formalin-fixed paraffin-embedded archival tissue. (2013)
  • Author(s): Nakao K, Oikawa M, Arai J, Mussazhanova Z, Kondo H, Shichijo K, Nakashima M, Hayashi T, Yoshiura K, Hatachi T, Nagayasu T
  • Journal Title: Diagnostic Molecular Pathology Volume: 22 Issue: 3 Pages: 174-180
  • DOI: 10.1097/pdm.0b013e31828191de
  • NAID: 120006986787
  • Peer Reviewed
• [Journal Article] A predictive factor of microarray comparative genomic hybridization analysis quality for formalin-fixed paraffin-embedded archival tissue. (2013)
  • Author(s): Kenjiro Nakao, Masahiro Oikawa (Corresponding auther), et.al.
  • Journal Title: Diagnostic Molecular Pathology Volume: なし
  • Peer Reviewed
• [Journal Article] Clinical and molecular analysis of synchronous double lung cancers (2012)
  • Author(s): Arai J, Tsuchiya T, Oikawa M, Mochinaga K, Hayashi T, Yoshiura K, Tsukamoto K, Yamasaki N, Matsumoto K, Miyazaki T, Nagayasu T
  • Journal Title: Lung Cancer Volume: 77(2) Pages: 281-7
  • NAID: 120006985469
  • Peer Reviewed
• [Presentation] The impact of intra-tumoral heterogeneity on the prognosis of ER-positive/HER2-negative breast cancer (2014)
  • Author(s): Oikawa M, Ishida M, Nakamura Y, Nishimura S, Koga C, Saruwatari A, Igawa A, Akiyoshi S, Koi Y, Ohno S
  • Organizer: ASCO2014
  • Place of Presentation: シカゴ, 米国
• [Presentation] ER陽性/HER2 陰性乳癌におけるHER2 scoreの再評価 (2014)
  • Author(s): 及川将弘, 石田真弓, 中村吉昭, 西村純子, 古閑知奈美, 猿渡彰洋, 井川明子, 秋吉清百合, 厚井裕三子, 大野真司
  • Organizer: 第22回日本乳癌学会学術総会
  • Place of Presentation: 大阪
• [Presentation] ER陽性/HER2 陰性乳癌におけるIntra-tumoral heterogeneity が予後に与える影響の解析 (2014)
  • Author(s): 及川将弘, 石田真弓, 中村吉昭, 西村純子, 古閑知奈美, 猿渡彰洋, 井川明子, 秋吉清百合, 厚井裕三子, 大野真司
  • Organizer: 第114回日本外科学会定期学術集会
  • Place of Presentation: 京都
• [Presentation] The impact of intra-tumoral heterogeneity on the prognosis of ER-positive /HER2-negative breast cancer. (2014)
  • Author(s): Masahiro Oikawa, Mayumi Ishida, Yoshiaki Nakamura, Sumiko Nishimura, Chinami Koga, Akihiro Saruwatari, Akiko Igawa, Sayuri Akiyoshi, Yumiko Koi, Shinji Ohno
  • Organizer: ASCO2014
  • Place of Presentation: シカゴ、米国
• [Presentation] ER陽性/HER2陰性乳癌におけるHER2 scoreの再評価 (2014)
  • Author(s): 及川将弘、石田真弓、中村吉昭、西村純子、古閑知奈美、猿渡彰洋、井川明子、秋吉清百合、厚井裕三子、大野真司
  • Organizer: 第22回日本乳癌学会学術総会
  • Place of Presentation: 大阪
• [Presentation] ER陽性/HER2陰性乳癌におけるIntra-tumoral heterogeneity が予後に与える影響の解析 (2014)
  • Author(s): 及川将弘、石田真弓、中村吉昭、西村純子、古閑知奈美、猿渡彰洋、井川明子、秋吉清百合、厚井裕三子、大野真司
  • Organizer: 第114回日本外科学会定期学術集会
  • Place of Presentation: 京都
• [Presentation] A novel diagnostic method targeting genomic instability in breast intracystic tumors (2013)
  • Author(s): Yano H, Oikawa M, Matsumoto M, Otsubo R, Shibata K, Hayashi T, Abe K, Kinoshita N, Nagayasu T
  • Organizer: SABCS2013
  • Place of Presentation: サンアントニオ, 米国
• [Presentation] 両悪性困難な乳腺腫瘍に対するFNAC-aCGH 法の有用性の検討 (2013)
  • Author(s): 及川将弘, 矢野洋, 松本恵, 大坪竜太, 林徳真吉, 安倍邦子, 木下直江, 永安武
  • Organizer: 第21回日本乳癌学会学術総会
  • Place of Presentation: 浜松
• [Presentation] 乳腺嚢胞内腫瘍に対する, ゲノム不安定性を指標にした良悪性鑑別法の検討 (2013)
  • Author(s): 及川将弘, 矢野洋, 松本恵, 大坪竜太, 林徳真吉, 安倍邦子, 木下直江, 黒木祥司, 及川達司, 永安武
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] A novel diagnostic method targeting genomic instability in breast intracystic tumors. (2013)
  • Author(s): H.Yano, M.Oikawa, M.Matsumoto, R.Otsubo, K.Shibata, T.Hayashi, K.Abe, N.Kinoshita, T. Nagayasu.
  • Organizer: SABCS2013
  • Place of Presentation: サンアントニオ、米国
• [Presentation] 良悪性診断困難な乳腺腫瘍に対するFNAC-aCGH法の有用性の検討 (2013)
  • Author(s): 及川将弘、矢野洋、松本恵、大坪竜太、林徳真吉、安倍邦子、木下直江、永安武
  • Organizer: 第21回日本乳癌学会学術総会
  • Place of Presentation: 浜松
• [Presentation] 乳腺嚢胞内腫瘍に対する、ゲノム不安定性を指標にした良悪性鑑別法の検討 (2013)
  • Author(s): 及川将弘、矢野洋、松本恵、大坪竜太、林徳真吉、安倍邦子、木下直江、黒木祥司、及川達司、永安武
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡
• [Presentation] Utilization of formalin-fixed paraffin-embedded archival tissue for cytogenetic analysis of microarray-comparative genomic hybridization (2012)
  • Author(s): Oikawa M, Arai J, Kondo H, Nakashima M, Hayashi T, Yoshiura KI, Yano H, Nagayasu T
  • Organizer: ESMO2012
  • Place of Presentation: ウィーン, オーストリア
• [Presentation] 乳癌FFPE 標本を用いたアレイCGH 解析の精度管理 (2012)
  • Author(s): 及川将弘, 中尾健次郎, 柴田健一郎, 松本恵, 矢野洋, 木下直江, 安倍邦子, 林徳真吉, 蔵重智美, 三浦史郎, 中島正洋, 永安武
  • Organizer: 第20回日本乳癌学会学術総会
  • Place of Presentation: 熊本
• [Presentation] HR 陽性・HER2 陰性乳癌におけるKi67 の検討 (2012)
  • Author(s): 中尾健次郎, 及川将弘, 柴田健一郎, 松本恵, 林徳真吉, 永安武
  • Organizer: 第20回日本乳癌学会学術総会
  • Place of Presentation: 熊本
• [Presentation] Utilization of formalin-fixed paraffin-embedded archival tissue for cytogenetic analysis of microarray-comparative genomic hybridization. (2012)
  • Author(s): Oikawa M, Arai J, Kondo H, Nakashima M, Hayashi T, Yoshiura K I, Yano H, Nagayasu T.
  • Organizer: EUROPEAN SOCIETY FOR MEDICAL ONCOLOGY, 2012 Congress
  • Place of Presentation: Viena
• [Presentation] 乳癌FFPE標本を用いたアレイCGH解析の精度管理 (2012)
  • Author(s): 及川 将弘(長崎大学 大学院腫瘍外科), 中尾 健次郎, 柴田 健一郎, 松本 恵, 矢野 洋, 木下 直江, 安倍 邦子, 林 徳真吉, 蔵重 智美, 三浦 史郎, 中島 正洋, 永安 武
  • Organizer: 第20回日本乳癌学会総会
  • Place of Presentation: 熊本市
• [Presentation] HR陽性・HER2陰性乳癌におけるKi67の検討 (2012)
  • Author(s): 中尾 健次郎及川 将弘, 柴田 健一郎, 松本 恵, 林 徳真吉, 永安 武
  • Organizer: 第20回日本乳癌学会総会
  • Place of Presentation: 熊本市