Subtype-specific metabolic characteristics and tailor-made treatment strategy in breast cancer by metabolome analysis

• Project/Area Number: 24791394
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General surgery
• Research Institution: Keio University
• Principal Investigator: MATSUMOTO Akiko 慶應義塾大学, 医学部, 助教 (70573418)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 乳癌 / メタボローム解析 / MALDI-MS / MALDI-IMS / サブタイプ

Research Abstract

Currently treatments are decided based on subtypes in breast cancer, however, metabolic alterations of subtypes remain to be elucidated.
Here, we analyzed subtype-specific metabolic alterations by using clinical specimens and cell lines of breast cancer. The most different metabolic characteristics were observed between luminal and triple negative type when we analyzed aspiration biopsy specimens by CE-MS. We also prepared tumor tissue which was obtained from transplanting cell lines of human breast cancer into immunodeficient mouse. As a result, we revealed distinctive metabolic patterns according to subtypes when we use MALDI-IMS.
Our results provided novel insights into tailor-made treatment for breast cancer using metabolome analysis by the leading edge of measurement technology.