| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2014: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Outline of Final Research Achievements |
An increase in regulatory T cells (Tregs) is observed in tumor microenvironments. Since it was suggested that Tregs showed a lower sensitivity toward oxidative stress in comparison with conventional T cells (Tcon), we investigated the H(2)O(2) production and apoptosis of Tregs in esophageal cancer tissues, employing flow cytometric analysis and immunohistochemical analysis. The increased tumor-infiltrating Tregs coexisted with elevated H(2)O(2) production according to disease progression. The grade of apoptosis in Tregs was less pronounced than that in Tcon, and there was a positive correlation between H(2)O(2) production and the grade of apoptosis in Tcon, while there was no correlation between H(2)O(2) production and the grade of apoptosis in Tregs. Moreover, Tregs were less sensitive to H(2)O(2)-induced apoptosis compared with Tcon in vitro. As conclusions, the increased prevalence of tumor-infiltrating Tregs closely related to their lower sensitivity to H(2)O(2)-induced apoptosis.
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