Characterization of hepatic cancer stem cells with billiary cell marker
| Project/Area Number |
24791409
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Digestive surgery
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| Research Institution | Kyoto University |
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Principal Investigator |
KODAMA Sota 京都大学, 医学(系)研究科(研究院), その他 (10583875)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 再生医学 / 癌幹細胞 / 肝臓癌 |
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| Research Abstract |
In this study, we aimed to determine if cytokeratin 19 (CK19), a hepatic progenitor cell marker, can used as a CSC marker in HCC.
First, we transfected the CK19 promoter-driven enhanced green fluorescence protein gene into human HCC cell lines. Then we isolated CK19+ cells using fluorescence-activated cell sorting (FACS). Furthermore, xenotransplantation into non-obese diabetic/severe combined immunodeficiency mice revealed that CK19+ cells could reproduce themselves, differentiate into CK19- cells, and generate larger tumors at a higher frequency in vivo. These results indicated that CK19+ HCC cells possess CSC properties. And, immunohistochemistry analyses of 166 consecutive human HCC specimens resected at Kyoto University showed that CK19 expression is an independent predictor of postoperative recurrence.
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Report
(3 results)
Research Products
(8 results)
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[Journal Article] Comparative study of transplantation of hepatocytes at various differentiation stages into mice with lethal liver damage2012
Author(s)
Ryo Kamimura, Takamichi Ishii, Naoya Sasaki, Masatoshi Kajiwara, Takafumi Machimoto, Miwa Saito, Kohno K, Hirofumi Suemori, Norio Nakatsuji, Iwao Ikai, Kentaro Yasuchik, Shinji Uemoto
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Journal Title
Cell Transplant
Volume: 21(11)
Issue: 11
Pages: 2351-62
DOI
Related Report
Peer Reviewed
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