| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Research Abstract |
Serum expression of miRNAs selected by miRNA array was measured by quantitative RT-PCR in 68 patients with esophageal cancer who received cisplatin-based chemotherapy to examine the relationship between miRNA expression and response to chemotherapy. In vitro assays were conducted using esophageal cancer cells and normal fibroblasts (NOF) to investigate the mechanism of miRNA-induced chemoresistance. 18 miRNAs were different between responders and non-responders by miRNA array. Of these, high expression levels of miR-X correlated with poor response to chemotherapy. Although transfection of miR-X to cancer cells had no significant impact on chemosensitivity, esophageal cancer cells cultured in supernatant of miR-X-transfected NOF showed reduced chemosensitivity. miR-X-transfected NOF showed a-SMA expression. Our results indicated that miR-X is involved in resistance to chemotherapy in esophageal cancer, through transformation of NOF into cancer-associated fibroblasts.
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