Novel development in viral therapy targeting pancreatic cancer microenvironment – viral spread and transduction efficiency in pancreatic stroma

• Project/Area Number: 24791432
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Digestive surgery
• Research Institution: Kyushu University
• Principal Investigator: YASUI Takaharu 九州大学, 医学(系)研究科(研究院), 共同研究員 (60611283)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: hypoxia / stellate cells / 膵癌微小環境 / 膵星細胞 / 抗線維化薬 / 低酸素微小管環境 / 癌間質相互作用 / CTGF

Research Abstract

The achievements in the present study are as follows. (1) Pancreatic stellate cells cultured in hypoxic condition promote invasive ability of pancreatic cancer cells. (2) The invasive ability of pancreatic cancer cells in hypoxic condition is promoted by connective tissue growth factor (CTGF) secreted by pancreatic stellate cells. (3) Lower expression of CD90 is associated with good prognosis in surgically resected pancreatic cancer patients. (4) The migratory and invasive ability of pancreatic cancer cells are increased when co-cultured with CD146 knockdown pancreatic stellate cells.

Research Products

• [Journal Article] Pirfenidone inhibits desmoplasia of pancreatic cancer by regulating stellate cells. (2013)
  • Author(s): Shingo Kozono, Kenoki Ohuchida, Daiki Eguchi, Naoki Ikenaga, Kenji Fujiwara, Ming Zhao, Lin Cui, Koji Shindo, Kazuhiro Mizumoto, and Masao Tanaka
  • Journal Title: Cancer Research Volume: 73 Issue: 7 Pages: 2345-2356
  • DOI: 10.1158/0008-5472.can-12-3180
  • Peer Reviewed
• [Journal Article] Hypoxia enhances the interaction between pancreatic stellate cells and cancer cells via increased secretion of connective tissue growth factor. (2013)
  • Author(s): Eguchi D
  • Journal Title: Journal of Surgical Research Volume: 181 Pages: 225-233
  • Peer Reviewed
• [Journal Article] Different incretin responses after pancreatoduodenectomy and distal pancreatectomy (2012)
  • Author(s): Mori Y, Ohtsuka T, Tsutsumi K, Yasui T, Ueda J, Takahata S, Nakamura M, Tanaka M
  • Journal Title: Pancreas Volume: 41(3) Issue: 3 Pages: 455-60
  • DOI: 10.1097/mpa.0b013e3182319d7c
• [Journal Article] Analysis of lymph node metastasis in pancreatic neuroendocrine tumors (PNETs) based on the tumor size and hormonal production (2012)
  • Author(s): Tsutsumi K, Ohtsuka T, Mori Y, Fujino M, Yasui T, Aishima S, Takahata S, Nakamura M, Ito T, Tanaka M
  • Journal Title: J Gastroenterol Volume: 47(6) Issue: 6 Pages: 678-85
  • DOI: 10.1007/s00535-012-0540-0
  • NAID: 10030802703
• [Journal Article] Is cholecystectomy necessary after endoscopic treatment of bile duct stones in patients older than 80 years of age? (2012)
  • Author(s): Yasui T, Takahata S, Kono H, Nagayoshi Y, Mori Y, Tsutsumi K, Sadakari Y, Ohtsuka T, Nakamura M, Tanaka M
  • Journal Title: J Gastroenterol Volume: 47(1) Issue: 1 Pages: 65-70
  • DOI: 10.1007/s00535-011-0461-3
  • NAID: 10030295747
• [Presentation] The development of pancreatic cancer therapy based on regulating pancreatic cancer desmoplasia by targeting pancreatic Stellate Cells
  • Author(s): Shingo Kozono
  • Organizer: International Pancreatic Research Forum 2013
  • Place of Presentation: Sendai
  • Invited
• [Presentation] Regulation of Pancreatic Stellate cells for Treatment of Pancreatic cancer
  • Author(s): Kenoki Ohuchida
  • Organizer: The 6th International Conference and Commemorative Lecture of Pin-Wen Lin of Pancreatic Cancer
  • Place of Presentation: Taiwan
  • Invited
• [Presentation] 膵癌間質におけるCD90とα-SMA発現の意義
  • Author(s): 佐田政史
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡市
• [Presentation] Clinical Significance of Stromal CD90 and alpha-SMA Expression in Pancreatic Cancer
  • Author(s): Masafumi Sada
  • Organizer: American Pancreatic Association, 44th Annual Meeting
  • Place of Presentation: Miami, USA
• [Presentation] The investigation of CD146 in pancreatic cancer associated fibroblast
  • Author(s): 趙茗
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡市
• [Presentation] 膵星細胞は低酸素下でCTGFの分泌を介して膵癌の浸潤に寄与する
  • Author(s): 江口大樹
  • Organizer: 第112回日本外科学会定期学術集会
  • Place of Presentation: 千葉
• [Presentation] Pancreatic cancer cells in hypoxia enhance the motility of pancreatic stellate cells via increased secretion of vascular endothelial growth factor
  • Author(s): Eguchi D
  • Organizer: 2012 Joint Meeting of APA and IAP
  • Place of Presentation: Miami, USA