Molecular roles of xCT and XB130 in esophageal squamous cell carcinoma
Project/Area Number |
24791440
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Digestive surgery
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Research Institution | Kyoto Prefectural University of Medicine |
Principal Investigator |
SHIOZAKI Atsushi 京都府立医科大学, 医学(系)研究科(研究院), 助教 (40568086)
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Project Period (FY) |
2012-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2012: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 食道癌 / xCT / XB130 / 消化器外科学 / 食道外科学 / アダプター蛋白 / トランスポーター / 細胞周期 |
Research Abstract |
The aims of the present study were to determine the roles of xCT, cysteine/glutamate transporter in esophageal squamous cell carcinoma (ESCC) cells, its clinical significance and interaction with XB130, adaptor protein. Knocking-down (KD) of xCT decreased XB130 expression, and KD of XB130 increased xCT expression in ESCC cells. Immunoprecipitation revealed no interaction between xCT and XB130. In TE13 and KYSE170 cells, KD of xCT inhibited G1-S phase progression. Microarray analysis identified 1652 genes whose expression levels in TE13 cells were altered by the KD of xCT. Pathway analysis showed that the top-ranked pathway was the G1/S checkpoint regulation. Immunohistochemical staining showed that the xCT positivity rate was positively correlated with the Ki-67 labeling index. Further, its expression was an independent prognostic factor. These observations suggest that the expression of xCT in ESCC cells might affect the cell cycle and impact on the prognosis of ESCC patients.
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Report
(3 results)
Research Products
(111 results)
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[Journal Article] Role of the Na+/K+/2Cl- cotransporter NKCC1 in cell cycle progression in human esophageal squamous cell carcinoma.2014
Author(s)
Shiozaki A, Nako Y, Ichikawa D, Konishi H, Komatsu S, Kubota T, Fujiwara H, Okamoto K, Kishimoto M, Marunaka Y, Otsuji E.
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Journal Title
World J Gastroenterol
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] Middle and lower esophagectomy preceded by hand-assisted laparoscopic transhiatal approach for distal esophageal cancer.2014
Author(s)
Shiozaki A, Fujiwara H, Konishi H, Morimura R, Komatsu S, Murayama Y, Kuriu Y, Ikoma H, Kubota T, Nakanishi M, Ichikawa D, Okamoto K, Sakakura C, Otsuji E.
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Journal Title
Molecular and clinical oncology.
Volume: 2
Issue: 1
Pages: 31-37
DOI
Related Report
Peer Reviewed
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[Journal Article] Esophagectomy with gastric tube reconstruction for patients who previously underwent free jejunal transfer.2014
Author(s)
Shiozaki A, Fujiwara H, Konishi H, Ishimoto T, Morimura R, Murayama Y, Komatsu S, Kuriu Y, Ikoma H, Kubota T, Nakanishi M, Ichikawa D, Okamoto K, Sakakura C, Otsuji E.
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Journal Title
Esophagus.
Volume: 印刷中
Issue: 3
Pages: 267-271
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] xCT, component of cysteine/glutamate transporter, as an independent prognostic factor in human esophageal squamous cell carcinoma.2013
Author(s)
Shiozaki A, Iitaka D, Ichikawa D, Nakashima S, Fujiwara H, Okamoto K, Kubota T, Komatsu S, Kosuga T, Takeshita H, Shimizu H, Nako Y, Sasagawa H, Kishimoto M, Otsuji E.
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Journal Title
J Gastroenterol
Volume: 印刷中
Issue: 5
Pages: 853-863
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Prognostic Significance of p21 Expression in Patients with Esophageal Squamous Cell Carcinoma.2013
Author(s)
Shiozaki A, Nakashima S, Ichikawa D, Fujiwara H, Konishi H, Komatsu S, Kubota T, Okamoto K, Iitaka D, Shimizu H, Nako Y, Takemoto K, Kishimoto M, Otsuji E.
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Journal Title
Anitcancer Research
Volume: 33
Pages: 4329-4335
Related Report
Peer Reviewed
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[Journal Article] Anit-phosphohistone H3 as an independent prognostic factor in human esophageal squamous cell carcinoma.2013
Author(s)
Nakashima S, Shiozaki A, Ichikawa D, Komatsu S, Konishi H, Iitaka D, Kubota T, Fujiwara H, Okamoto K, Kishimoto M, Otsuji E.
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Journal Title
Anticancer Research
Volume: 33
Pages: 461-468
Related Report
Peer Reviewed
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[Journal Article] E2F5 as an independent prognostic factor in esophageal squamous cell carcinoma.2013
Author(s)
Ishimoto T, Shiozaki A, Ichikawa D, Fujiwara H, Konishi H, Komatsu S, Kubota T, Okamoto K, Nakashima S, Shimizu H, Nako Y, Kishimoto M, Otsuji E.
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Journal Title
Anticancer Research
Volume: 33
Pages: 5415-5420
Related Report
Peer Reviewed
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[Journal Article] Novel technique for dissection of subcarinal and main bronchial lymph nodes using a laparoscopic transhiatal approach for esophageal cancer.2013
Author(s)
Shiozaki A, Fujiwara H, Konishi H, Morimura R, Murayama Y, Komatsu S, Kuriu Y, Ikoma H, Kubota T, Nakanishi M, Ichikawa D, Okamoto K, Sakakura C, Otsuji E.
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Journal Title
Anticancer Research
Volume: 33
Pages: 2577-2585
Related Report
Peer Reviewed
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[Journal Article] Anti-Phosphohistone H3 as an independent prognostic factor in human esophageal squamous cell carcinoma.2013
Author(s)
Nakashima S, Shiozaki A, Ichikawa D, Komatsu S, Konishi H, Iitaka D, Kubota T, Fujiwara H, Okamoto K, Kishimoto M, Otsuji E.
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Journal Title
Anticancer Res
Volume: 33
Pages: 461-467
Related Report
Peer Reviewed
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[Journal Article] Novel technique for dissection of subcarinal and main bronchus lymph nodes using a laparoscopic transhiatal approach for esophageal cancer2013
Author(s)
Shiozaki A, Fujiwara H, Konishi H, Morimura R, Murayama Y, Komatsu S, Kuriu Y, Ikoma H, Kubota T, Nakanishi M, Ichikawa D, Okamoto K, Sakakura C, and Otsuji E
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Journal Title
Anticancer Res
Volume: 印刷中
Related Report
Peer Reviewed
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[Journal Article] Hand-assisted laparoscopic transhiatal approach for mediastinal esophageal duplication cyst resection.2012
Author(s)
Shiozaki A, Fujiwara H, Murayama Y, Komatsu S, Kuriu Y, Ikoma H, Nakanishi M, Ichikawa D, Okamoto K, Ochiai T, Otsuji E.
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Journal Title
Esophagus
Volume: 9
Issue: 4
Pages: 247-251
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Ki-67 labeling index as an independent prognostic factor in human esophageal squamous cell carcinoma.2012
Author(s)
Sasagawa H, Shiozaki A, Iitaka D, Ichikawa D, Komatsu S, Fujiwara H, Okamoto K, Nakashima S, Kinoshita O, Murayama Y, Kuriu Y, Ikoma H, Nakanishi M, Ochiai T, Kokuba Y, Otsuji E.
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Journal Title
Esophagus
Volume: 9
Issue: 4
Pages: 195-202
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Blockade of Chloride Ion Transport Enhances the Cytocidal Effect of Hypotonic Solution in Gastric Cancer Cells2011
Author(s)
Iitaka D, Shiozaki A, Ichikawa D, Kosuga T, Komatsu S, Okamoto K, Fujiwara H, Ishii H, Nakahari T, Marunaka Y, Otsuji E
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Journal Title
J Surg Res
Volume: 176
Issue: 2
Pages: 524-34
DOI
Related Report
Peer Reviewed
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[Presentation] Roles of XB130, a novel adaptor protein, in esophageal squamous cell carcinoma2012
Author(s)
A. Shiozaki, T. Kosuga, H. Fujiwara, D. Ichikawa, S. Komatsu, K. Okamoto, Y. Murayama, Y. Kuriu, H. Ikoma, M. Nakanishi, T. Ochiai, Y. Kokuba, M. Liu, E. Otsuji
Organizer
13th World Congress of the International Society for Diseases of the Esophagus
Place of Presentation
Venice
Related Report
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[Presentation] Roles of xCT, cysteine/glutamate transporter, in esophageal squamous cell carcinoma2012
Author(s)
A. Shiozaki, D. Iitaka, H. Fujiwara, D. Ichikawa, S. Komatsu, K. Okamoto, Y. Murayama, Y. Kuriu, H. Ikoma, M. Nakanishi, T. Ochiai, Y. Kokuba, E. Otsuji
Organizer
13th World Congress of the International Society for Diseases of the Esophagus
Place of Presentation
Venice
Related Report
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[Presentation] Clinical significance of Ki-67 labeling index in patients with esophageal squamous cell carcinoma2012
Author(s)
A. Shiozaki, D. Iitaka, H. Fujiwara, D. Ichikawa, S. Komatsu, K. Okamoto, Y. Murayama, Y. Kuriu, H. Ikoma, M. Nakanishi, T. Ochiai, Y. Kokuba, E. Otsuji
Organizer
13th World Congress of the International Society for Diseases of the Esophagus
Place of Presentation
Venice
Related Report
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[Presentation] The role of Claudin-1 as a mediator of TNF-alpha associated with cell migration2012
Author(s)
Hiroki Shimizu, Atsushi Shiozaki, Daisuke Ichikawa, Hirotaka Konishi, Shuhei Komatsu, Takeshi Kubota, Hitoshi Fujiwara, Kazuma Okamoto, Toshiya Ochiai, Kazuyo Naito, Mingyao Liu, Eigo Otsuji
Organizer
日本癌学会学術総会
Place of Presentation
札幌
Related Report
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[Presentation] XB130 regulates expressions of tumor suppressor microRNAs in thyroid cancer cells.2012
Author(s)
Takeshita H, Shiozaki A, Ichikawa D, Komatsu S, Konishi H, Murayama Y, Kuriu Y, Ikoma H, Kubota T, Nakanishi M, Okamoto K, Fujiwara H, Ochiai T, Kokuba Y, Liu M, Otsuji E
Organizer
日本癌学会学術総会
Place of Presentation
札幌
Related Report
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