• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Elucidation of lumican and development of the new therapeutic drug for gastrointestinal neuroendocrine carcinoma

Research Project

Project/Area Number 24791450
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Digestive surgery
Research InstitutionNippon Medical School

Principal Investigator

SHINJI Seiichi  日本医科大学, 医学部, 助教 (80409193)

Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2014: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywords消化管神経内分泌癌 / がん幹細胞 / 細胞外基質 / lumican / 消化管神経内分泌腫瘍 / 癌幹細胞
Outline of Final Research Achievements

We hypothesized that NEC contains a subpopulation of cancer stem cells (CSCs), we examined expression and role of lumican and CSC marker in gastrointestinal neuroendocrine carcinoma (NEC). Cytoplasm and tumor stroma were positively stained for lumican by immunohistochemcal analysis. We used established cell line (NENMS-1) derived from ascending colon NEC cancer patient. Src,CD133 and lumican mRNA were over expressed in sphere than wild by quantitative RT-PCR analyses. Lumican-overexpressing NENMS-1 was reduced cell growth, sphere formation and cell migration compared with wild and Control-GFP. CD133 and lumican mRNA expression were high in positive compartmentation by aldefluor assay. From the above, it was suggested lumican may become a CSC marker for gastrointestinal NEC.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (3 results)

All 2014 Other

All Presentation (3 results)

  • [Presentation] 消化管神経内分泌癌におけるがん幹細胞マーカーの同定とlumicanの役割2014

    • Author(s)
      進士誠一
    • Organizer
      JDDW2014
    • Place of Presentation
      神戸
    • Year and Date
      2014-10-23 – 2014-10-26
    • Related Report
      2014 Annual Research Report
  • [Presentation] 消化管神経内分泌癌培養細胞の樹立とがん幹細胞マーカーの同定2014

    • Author(s)
      進士誠一
    • Organizer
      第114回日本外科学会定期学術集会
    • Place of Presentation
      京都
    • Year and Date
      2014-04-03 – 2014-04-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] 大腸神経内分泌癌におけるがん幹細胞マーカー

    • Author(s)
      進士誠一
    • Organizer
      第80回大腸癌研究会
    • Place of Presentation
      東京
    • Related Report
      2013 Research-status Report

URL: 

Published: 2013-05-31   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi