microRNAs biomarkers for non-small cell lung cancer

• Project/Area Number: 24791455
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Thoracic surgery
• Research Institution: The University of Tokyo
• Principal Investigator: SANO Atsushi 東京大学, 医学部附属病院, 登録研究員 (20569834)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 腫瘍外科学 / 肺癌 / マイクロRNA / エピジェネティクス / DNAメチル化 / ヒストン修飾 / ＤＮＡメチル化 / マイクロＲＮＡ

Outline of Final Research Achievements

By combining in silico and experimental approaches, we identified miR-34b and miR-126 to be silenced by DNA methylation in non-small cell lung cancer (NSCLC). The DNA methylation of miR-34b was significantly associated with lympatic invation in primary NSCLC. We also found that miR-139 was frequently suppressed by histone methylation of its host gene PDE2A in NSCLC. We also found that decreased miR-139 expression was significantly associated with distant lymph node metastasis and histological invasiveness in primary NSCLC. These results suggest that epigentic silencing of miRNAs may contribute to malignant phenotype of NSCLC.

Research Products

• [Journal Article] Alternative polyadenylation is associated with lower expression of PABPN1 and poor prognosis in non-small cell lung cancer. (2014)
  • Author(s): Ichinose J, Watanabe K, Sano A, Nagase T, Nakajima J, Fukayama M, Yatomi Y, Ohishi N, Takai D.
  • Journal Title: Cancer Sci Volume: 105 Issue: 9 Pages: 1135-1141
  • DOI: 10.1111/cas.12472
  • Peer Reviewed / Open Access
• [Journal Article] Virtual Bronchoscopy Using OsiriX. (2014)
  • Author(s): Sano A, Tsuchiya T.
  • Journal Title: J Bronchology Interv Pulmonol. Volume: 21(2) Issue: 2 Pages: 113-116
  • DOI: 10.1097/lbr.0000000000000051
  • Peer Reviewed
• [Journal Article] Outpatient Drainage Therapy with a Thoracic Vent for Traumatic Pneumothorax due to Bull Attack. (2014)
  • Author(s): Sano A, Tsuchiya T, Nagano M.
  • Journal Title: Korean J Thorac Cardiovasc Surg. Volume: 47(6) Issue: 6 Pages: 563-565
  • DOI: 10.5090/kjtcs.2014.47.6.563
  • Peer Reviewed
• [Journal Article] Recurrent Pneumothorax Related to Swyer-James Syndrome (2014)
  • Author(s): Sano A, Fukami T, Murakawa T, Nakajima J.
  • Journal Title: Annals of Thoracic and Cardiovascular Surgery Volume: 20 Issue: Supplement Pages: 539-541
  • DOI: 10.5761/atcs.cr.12.02102
  • NAID: 130003377823
  • ISSN: 1341-1098, 2186-1005
  • Peer Reviewed
• [Journal Article] Thymoma with ring calcification. (2014)
  • Author(s): Sano A, Kawashima M.
  • Journal Title: Ann Thorac Surg. Volume: 98(6) Issue: 6 Pages: 2202-2204
  • DOI: 10.1016/j.athoracsur.2014.01.084
  • Peer Reviewed
• [Journal Article] Sternal closure with absorbable pins and cords in general thoracic surgery. (2014)
  • Author(s): Kawashima M, Sano A, Tanaka M.
  • Journal Title: Surg Today.
  • Peer Reviewed
• [Journal Article] 左肺底区より出血した気管支動脈蔓状血管腫の1例. (2014)
  • Author(s): 佐野 厚，土屋 武弘，川島 光明，浅井 威信，田代 研.
  • Journal Title: 気管支学 Volume: 36(2) Pages: 138-141
  • NAID: 110009807526
  • Peer Reviewed
• [Journal Article] 後側方切開第10肋間開胸で切除した縦隔神経鞘腫． (2014)
  • Author(s): 佐野 厚，川島 光明．
  • Journal Title: 胸部外科 Volume: 67(6) Pages: 459-462
  • Peer Reviewed
• [Journal Article] 横隔膜交通症に対する胸腔鏡下手術の工夫． (2014)
  • Author(s): 土屋 武弘，佐野 厚，深見 武史，柳 麻衣, 吉浦 辰徳, 小林 竜．
  • Journal Title: 胸部外科 Volume: 67(11) Pages: 963-966
• [Journal Article] Outcomes of combined modality therapy for patients with stage III or IV mediastinal malignant germ cell tumors (2013)
  • Author(s): Hideki Kuwano
  • Journal Title: Surgery Today Volume: - Issue: 3 Pages: 499-504
  • DOI: 10.1007/s00595-013-0562-0
  • Peer Reviewed
• [Journal Article] Recurrent Pneumothorax Related to Swyer-James Syndrome. (2013)
  • Author(s): Sano A, Fukami T, Murakawa T, Nakajima J.
  • Journal Title: Annals of Thoracic and Cardiovascular Surgery Volume: Feb 15
  • NAID: 130003377823
  • Peer Reviewed
• [Journal Article] Chronic tension empyema. (2013)
  • Author(s): Sano A, Murakawa T, Nakajima J.
  • Journal Title: Asian Cardiovascular and Thoracic Annals Volume: 21 Issue: 1 Pages: 93-96
  • DOI: 10.1177/0218492312450020
  • Peer Reviewed
• [Journal Article] The ground glass opacity component can be eliminated from the T-factor assessment of lung adenocarcinoma. (2013)
  • Author(s): Tomohiro Murakawa, Chihiro Konoeda,Takuya Ito, Yuta Inoue, Atsushi Sano, Kazuhiro Nagayama, Jun Nakajima.
  • Journal Title: European journal of cardio-thoracicsurgery. Volume: 43(5) Issue: 5 Pages: 925-932
  • DOI: 10.1093/ejcts/ezs467
  • Peer Reviewed
• [Journal Article] Epithelial to Mesenchymal Transition in Murine Tracheal Allotransplantation: An Immunohistochemical Observation. (2013)
  • Author(s): Chihiro Konoeda
  • Journal Title: Transplantation Proceedings Volume: - Issue: 5 Pages: 1797-1801
  • DOI: 10.1016/j.transproceed.2012.11.024
  • Peer Reviewed
• [Journal Article] Chest wall deformity found in patients with primary spontaneous pneumothorax. (2013)
  • Author(s): Saita K, Murakawa T, Kawano H, Sano A, Nagayama K, Nakajima J.
  • Journal Title: Asian Cardiovascular and Thoracic Annals Volume: 21 Issue: 5 Pages: 582-587
  • DOI: 10.1177/0218492312467174
  • Peer Reviewed
• [Journal Article] CT画像からの3次元気管支画像の作成 (2013)
  • Author(s): 佐野 厚, 瀬尾 拡史, 後藤 悌, 吉川 充浩, 中川 恵一.
  • Journal Title: 呼吸と循環 Volume: 61 Pages: 970-974
  • NAID: 40019809975
  • Peer Reviewed
• [Journal Article] 発症2日後に急性膿胸を発症した自然気胸の1例 (2013)
  • Author(s): 佐野 厚, 土屋 武弘.
  • Journal Title: 日本臨床外科学会雑誌 Volume: 74 Pages: 2116-2118
  • NAID: 10031203071
  • Peer Reviewed
• [Journal Article] 肺癌術後16日目に肺血栓塞栓症を発症した1例 (2013)
  • Author(s): 土屋 武弘, 佐野 厚, 深見 武史.
  • Journal Title: 胸部外科 Volume: 66 Pages: 855-857
  • Peer Reviewed
• [Journal Article] 肺気腫と間質性肺炎を伴った原発性肺癌手術例の術後合併症についての検討 (2013)
  • Author(s): 佐野 厚, 三枝 良輔, 高橋 剛史, 一瀬 淳二, 此枝 千尋, 井上 雄太, 長山 和弘, 村川 知弘, 中島 淳.
  • Journal Title: 臨床呼吸生理 Volume: 45 Pages: 35-37
  • Peer Reviewed
• [Journal Article] 左側奇静脈食道陥凹様構造に形成されたブラ破裂による続発性自然気胸 (2013)
  • Author(s): 川島 光明, 村川 知弘, 高橋 剛史, 此枝 千尋, 井上 雄太, 佐野 厚, 中島 淳.
  • Journal Title: 胸部外科 Volume: 66 Pages: 563-565
  • Peer Reviewed
• [Journal Article] The ground glass opacity component can be eliminated from the T-factor assessment of lung adenocarcinoma (2013)
  • Author(s): Murakawa T, Konoeda C, Ito T, Inoue Y, Sano A, Nagayama K, Nakajima J.
  • Journal Title: European Journal of Cardiothoracic Surgery Volume: 42
  • Peer Reviewed
• [Journal Article] Survival after extended thymectomy for thymoma (2012)
  • Author(s): Sakamoto M, Murakawa T, Konoeda C, Inoue Y, Kitano K, Sano A, Fukayama M, Nakajima J
  • Journal Title: European Journal of Cardiothoracic Surgery Volume: 41 Issue: 3 Pages: 623-627
  • DOI: 10.1093/ejcts/ezr026
  • Peer Reviewed
• [Journal Article] 骨肉腫治療後二次癌として発症した17歳の原発性肺癌 (2012)
  • Author(s): 此枝 千尋, 村川 知弘, 佐野 厚, 川島 光明, 中島 淳
  • Journal Title: 胸部外科 Volume: 65 Pages: 1127-1130
  • NAID: 40019497437
  • Peer Reviewed
• [Journal Article] CT上スリガラス状陰影を呈した肺毛細血管腫 (2012)
  • Author(s): 嶋田 喜文, 村川 知弘, 佐野 厚, 深見 武史, 吉田 幸弘, 井上 雄太, 森田 茂樹, 深山 正久, 中島 淳
  • Journal Title: 胸部外科 Volume: 65 Pages: 1038-1043
  • NAID: 40019467093
  • Peer Reviewed
• [Presentation] 後側方切開第10肋間開胸で切除した縦隔神経鞘腫． (2014)
  • Author(s): 佐野 厚，川島 光明．
  • Organizer: 第76回日本臨床外科学会総会
  • Place of Presentation: 郡山
  • Year and Date: 2014-11-22
• [Presentation] 二次救急医療機関における外傷性気胸症例の検討． (2014)
  • Author(s): 佐野 厚，川島 光明，乾 雅人．
  • Organizer: 第76回日本臨床外科学会総会
  • Place of Presentation: 郡山
  • Year and Date: 2014-11-20
• [Presentation] 輪状石灰化を伴った胸腺腫の一例． (2014)
  • Author(s): 佐野 厚，川島 光明．
  • Organizer: 第55回日本肺癌学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-11-15
• [Presentation] 非小細胞肺癌におけるmRNA 3’非翻訳領域の短縮と腫瘍の悪性度の関係 (2014)
  • Author(s): 一瀬 淳二、渡辺 広祐、佐野 厚、村川 知弘、高井 大哉、中島 淳
  • Organizer: 第55回日本肺癌学会総会
  • Place of Presentation: 京都
  • Year and Date: 2014-11-14
• [Presentation] 単孔式胸腔鏡手術後の再発気胸に対する胸腔鏡下手術の経験． (2014)
  • Author(s): 佐野 厚，川島 光明，乾 雅人．
  • Organizer: 第18回日本気胸・嚢胞性肺疾患学会総会
  • Place of Presentation: 福岡
  • Year and Date: 2014-09-05
• [Presentation] VIRTUAL BRONCHOSCOPY USING OsiriX. (2014)
  • Author(s): Sano A, Tsuchiya T, Kawashima M, Tashiro K, Hoshino M, Hara H, Fukuda T.
  • Organizer: 18th World Congress of Bronchology and Interventional Pulmonology.
  • Place of Presentation: 京都
  • Year and Date: 2014-04-14
• [Presentation] OsiriXによる仮想気管支鏡の作成． (2014)
  • Author(s): 佐野 厚
  • Organizer: 第37回日本呼吸器内視鏡学会学術集会
  • Place of Presentation: 京都
  • Year and Date: 2014-04-14
• [Presentation] Loss of PABPN1 and alternative polyadenylation yield poor prognosis in non-small cell lung cancer. (2014)
  • Author(s): Ichinose J, Watanabe K, Sano A, Nagase T, Nakajima J, Fukayama M, Yatomi Y, Ohishi N, Takai D.
  • Organizer: 3rd AACR-IASLC Joint Conference on the Molecular Origins of Lung Cancer
  • Place of Presentation: Sandiego, CA, USA.
• [Presentation] Virtual Bronchoscopy and Virtual Bronchoscopic Lung Biopsy using OsiriX. (2013)
  • Author(s): Sano A, Tsuchiya T.
  • Organizer: 15th World Conference on Lung Cancer
  • Place of Presentation: Sydney, Australia.
• [Presentation] 3DCTと3Dプリンタを用いた気管支実物大模型の作成 (2013)
  • Author(s): 佐野 厚, 浅井 偉信, 田代 研.
  • Organizer: 第36回日本呼吸器内視鏡学会
  • Place of Presentation: 埼玉
• [Presentation] Thoracic Ventによる外来ドレナージを行った家畜関連外傷性気胸の1例 (2013)
  • Author(s): 佐野 厚, 土屋 武弘, 長野 匡晃.
  • Organizer: 第17回日本気胸・嚢胞性肺疾患学会総会
  • Place of Presentation: 東京
• [Presentation] 両側多発肺動静脈瘻に対する一期的両側胸腔鏡下手術例 (2013)
  • Author(s): 佐野 厚, 土屋 武弘.
  • Organizer: 第75回日本臨床外科学会総会
  • Place of Presentation: 名古屋
• [Presentation] 脳死肺葉移植における左肺の肺葉の選択 (2012)
  • Author(s): 佐野 厚、長山 和弘、村川 知弘、中島 淳
  • Organizer: 第29回日本呼吸器外科学会総会
  • Place of Presentation: 秋田
• [Presentation] OsiriXによる仮想気管支鏡を用いた気管支鏡検査で診断された肺結核の1例 (2012)
  • Author(s): 佐野 厚、川島 光明、齊藤 未央、高橋 剛史、三枝 良輔、一瀬 淳二、此枝 千尋、井上 雄太、長山 和弘、村川 知弘、中島 淳
  • Organizer: 第141回 日本呼吸器内視鏡学会関東支部会
  • Place of Presentation: 東京
• [Presentation] CT画像からの3D気管支モデルの作成 (2012)
  • Author(s): 佐野 厚、瀬尾 拡史、後藤 悌、吉川 充浩、中川 恵一
  • Organizer: 第143回 日本呼吸器内視鏡学会関東支部会
  • Place of Presentation: 東京