Molecular analysis of lung cancer stem cell related gene, SMCP

• Project/Area Number: 24791467
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Thoracic surgery
• Research Institution: Sapporo Medical University
• Principal Investigator: TAKAHASHI Akari 札幌医科大学, 医学部, 研究員 (80457697)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 肺がん / がん幹細胞 / SMCP / 腫瘍起始能 / 造腫瘍

Outline of Final Research Achievements

We isolated lung cancer stem-like cells(CSCs)/cancer-initiating cells(CICs) by side population assay. Transcriptome anslysis revealed that a novel cancer-testis gene SMCP is preferentially expressed in lung CSCs/CICs. Gene overexpression and gene knockdown assay using siRNAs revealed that SMCP has a role in the tumorigenicity of lung CSCs/CICs. These observations indicate that novel cancer testis gene SMCP might be a promising target for CSC/CIC-targeting therapy.

Research Products

• [Journal Article] Cancer-Associated Oxidase ERO1-α Regulates the Expression of MHC Class I Molecule via Oxidative Folding (2015)
  • Author(s): Kukita K, Tamura Y, Tanaka T, Kajiwara T, Kutomi G, Saito K, Okuya K, Takaya A, Kanaseki T, Tsukahara T, Hirohashi Y, Torigoe T, Furuhata T, Hirata K, Sato N.
  • Journal Title: J Immunol. Volume: Epub
  • Peer Reviewed
• [Journal Article] Heat shock protein 90 targets a chaperoned peptide to the static early endosome for efficient cross-presentation by human dendritic cells (2015)
  • Author(s): Tsutomu Tanaka, Koichi Okuya, Goro Kutomi, Akari Takaya, Toshimitsu Kajiwara, Takayuki Kanaseki, Tomohide Tsukahara, Yoshihiko Hirohashi, Toshihiko Torigoe, Koichi Hirata, Yoshiharu Okamoto, Noriyuki Sato, Yasuaki Tamura
  • Journal Title: Cancer Science Volume: 106 Issue: 1 Pages: 18-24
  • DOI: 10.1111/cas.12570
  • NAID: 120005593448
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] PolyI:C and mouse survivin artificially embedding human 2B peptide induce a CD4+ T cell response to autologous survivin in HLA-A*2402 transgenic mice. (2015)
  • Author(s): Kasamatsu J, Takahashi S, Azuma M, Matsumoto M, Morii-Sakai A, Imamura M, Teshima T, Takahashi A, Hirohashi Y, Torigoe T, Sato N, Seya T.
  • Journal Title: Immunobiology Volume: 220 Issue: 1 Pages: 74-82
  • DOI: 10.1016/j.imbio.2014.08.017
  • Peer Reviewed
• [Journal Article] Heat shock protein DNAJB8 is a novel target for immunotherapy of colon cancer-initiating cells. (2014)
  • Author(s): Morita R, Nishizawa S, Torigoe T, Takahashi A, Tamura Y, Tsukahara T, Kanaseki T, Sokolovskaya A, Kochin V, Kondo T, Hashino S, Asaka M, Hara I, Hirohashi Y, Sato N.
  • Journal Title: Cancer Sci Volume: 105 Issue: 4 Pages: 389-395
  • DOI: 10.1111/cas.12362
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ectopically expressed variant form of sperm mitochondria-associated cysteine-rich protein augments tumorigenicity of the stem cell population of lung adenocarcinoma cells. (2013)
  • Author(s): Takahashi A, Hirohashi Y, Torigoe T, Tamura Y, Tsukahara T, Kanaseki T, Kochin V, Saijo H, Kubo T, Nakatsugawa M, Asanuma H, Hasegawa T, Kondo T, Sato N.
  • Journal Title: PLoS One Volume: 8 Issue: 11 Pages: e69095-e69095
  • DOI: 10.1371/journal.pone.0069095
  • Peer Reviewed
• [Journal Article] Ovarian cancer stem cells are enriched in side population and aldehyde dehydrogenase bright overlapping population. (2013)
  • Author(s): Yasuda K, Torigoe T, Morita R, Kuroda T, Takahashi A, Matsuzaki J, Kochin V, Asanuma H, Hasegawa T, Saito T, Hirohashi Y, Sato N.
  • Journal Title: PLoS One Volume: 8(8) Issue: 8 Pages: e68187-e68187
  • DOI: 10.1371/journal.pone.0068187
  • Peer Reviewed
• [Journal Article] Small proline-rich protein-1B is overexpressed in human oral squamous cell cancer stem-like cells and is related to their growth through activation of MAP kinase signal. (2013)
  • Author(s): Michifuri Y, Hirohashi Y, Torigoe T, Miyazaki A, Fujino J, Tamura Y, Tsukahara T, Kanaseki T, Kobayashi J, Sasaki T, Takahashi A, Nakamori K, Yamaguchi A, Hiratsuka H, Sato N.
  • Journal Title: Biochem Biophys Res Commun Volume: 439 Issue: 1 Pages: 96-102
  • DOI: 10.1016/j.bbrc.2013.08.021
  • Peer Reviewed
• [Journal Article] AIDH1-high ovarian cancer stem-like cells can be isolated from serous and clear cell adenocarcinoma cells, and AIDH1 high expression is associated with poor prognosis. (2013)
  • Author(s): Kuroda T, Hirohashi Y, Torigoe T, Yasuda K, Takahashi A, Asanuma H, Morita R, Mariya T, Asano T, Mizuuchi M, Saito T, Sato N.
  • Journal Title: PLoS One Volume: 8(6) Issue: 6 Pages: e65158-e65158
  • DOI: 10.1371/journal.pone.0065158
  • Peer Reviewed
• [Journal Article] Preferential expression of cancer/testis genes in cancer stem-like cells : proposal of a novel sub-category, cancer/testis/stem gene. (2013)
  • Author(s): Yamada R, Takahashi A, Torigoe T, Morita R, Tamura Y, Tsukahara T, Kanaseki T, Kubo T, Watarai K, Kondo T, Hirohashi Y, Sato N.
  • Journal Title: Tissue Antigens. Volume: 81(6) Issue: 6 Pages: 428-34
  • DOI: 10.1111/tan.12113
  • Peer Reviewed
• [Journal Article] Prostate cancer stem-like cells/cancer-initiating cells have an autocrine system of hepatocyte growth factor. (2013)
  • Author(s): Nishida S, Hirohashi Y, Torigoe T, Inoue R, Kitamura H, Tanaka T, Takahashi A, Asanuma H, Masumori N, Tsukamoto T, Sato N.
  • Journal Title: Cancer Sci Volume: 104 Issue: 4 Pages: 431-6
  • DOI: 10.1111/cas.12104
  • Peer Reviewed
• [Journal Article] Expression and function of FERMT genes in colon carcinoma cells (2013)
  • Author(s): Kiriyama K, Hirohashi Y, Torigoe T, Kubo T, Tamura Y, Kanaseki T, Takahashi A, et al.
  • Journal Title: Anticancer Res Volume: 33 Pages: 167-73
  • Peer Reviewed
• [Journal Article] DNA methyltransferase 1 is essential for initiation of the colon cancers (2013)
  • Author(s): Morita R,Hirohashi Y,Suzuki H, et al
  • Journal Title: Experimental and Molecular Pathology Volume: 94 Issue: 2 Pages: 322-329
  • DOI: 10.1016/j.yexmp.2012.10.004
  • Peer Reviewed
• [Journal Article] Heat shock enhances the expression of cytotoxic granule proteins and augments the activities of tumor-associated antigen-specific cytotoxic T lymphocytes (2012)
  • Author(s): Takahashi A, Torigoe T, Tamura Y, Kanaseki T, Tsukahara T, Sasaki Y, Kameshima H, Tsuruma T, Hirata K, Tokino T, Hirohashi Y, Sato N
  • Journal Title: Cell Stress Chaperones Volume: 17 Issue: 6 Pages: 757-763
  • DOI: 10.1007/s12192-012-0348-0
  • Peer Reviewed
• [Presentation] Variant form of SMCP increases tumor initiation capacity of the stem cell population of lung adenocarcinoma cells (2013)
  • Author(s): Takahashi A, Torigoe T, Hirohashi Y, Tamura Y, Tsukahara T, Kanaseki T, Saijo H, Asanuma H, Sato N
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜
• [Presentation] Identification of the naturally processed peptide derived from FAM83B that is specifically expressed in cancer initiating cells (2013)
  • Author(s): Takahashi A, Kochin V, Kanaseki T, Hirohashi Y, Kawami S, Suzuki Y, Torigoe T, Sato N
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 幕張メッセ
• [Presentation] Single cell cloning of cancer stem cells revealed distinct plasticity among cancer cells
  • Author(s): 髙橋 あかり
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
• [Presentation] Moderate heat treatment enhances activities of human cytotoxic T lymphocytes
  • Author(s): 髙橋 あかり
  • Organizer: IHCO＆JCTM（The 11th International Congress of Hyperthermic Oncology (ICHO) & The 29th Japanese Congress of Thermal Medicine (JCTM)合同大会）
  • Place of Presentation: 京都