| Project/Area Number |
24791479
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Thoracic surgery
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| Research Institution | Fukuoka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
TSUNODA Toshiyuki 福岡大学, 医学部, 准教授 (70444817)
NABESHIMA Kazuki 福岡大学, 医学部, 教授 (40189189)
SHIRASAWA Senji 福岡大学, 医学部, 教授 (10253535)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Fiscal Year 2013: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2012: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 肺癌 / KRAS / micropapillary pattern / 微小浸潤癌 / 微小浸潤肺癌 / 患者血清サンプル / 腫瘍組織サンプル / 縦隔リンパ節サンプル / 変異KRAS / 末梢分泌型miRNA |
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| Outline of Final Research Achievements |
It was analyzed whether there was KRAS mutation in a neoplastic cell in 110 cases of lung cancer removal.The KRAS mutation was confirmed in 7 cases in 110 cases.The pathological special quality was compared between the KRAS mutation positive case and the negative case.We found out that there are a lot of cases with micropapillary pattern in a KRAS mutation positive case.
Patients with MPP has been reported with weak cell adhesion, prone to metastasis.We proved that a cell adhesion is weak by a colon cancer cell line with KRAS mutation, to prove it equally by a lung cancer cell, We have produced cells that suppress the expression of a mutant KRAS from lung cancer cell lines that have KRAS mutations. We schedule to extract and analyze miRNA in the culture fluid using the lung cancer cell which suppressed mutation KRAS.
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