Establishment of patient-derived non-small cell lung cancer xenografts as model for enrichment of cancer stem cell, and identification of lung cancer stem cell.

• Project/Area Number: 24791480
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Thoracic surgery
• Research Institution: 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所)
• Principal Investigator: NAKAGAWA Takayuki 地方独立行政法人宮城県立病院機構宮城県立がんセンター(研究所), がん幹細胞研究部, 研究員 (10626261)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 肺癌 / 癌幹細胞

Research Abstract

In order to develop the new therapeutic strategy for non-small cell lung cancer (NSCLC), I tried to establish patient-derived NSCLC xenografts on the NOG mice and explore the molecules involved in the characteristics of cancer stem cell. We established 15 passagable xenograft lines. A comparison of the histology of original sample with xenografted tumors revealed the similar histologic architecture of the primary and xenograft tumor. As cancer stem cell related molecule, I focused on large non-coding RNA HOTAIR. As expected, the expression of HOTAIR was found especially in aggressive NSCLC.

Research Products

• [Journal Article] Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer (2014)
  • Author(s): Nakagawa T, Endo H, Yokoyama M, Abe J, Tamai K, Tanaka N, Sato I, Takahashi S, Kondo T, Satoh K.
  • Journal Title: Biochem Biophy Res Commun Volume: 436 Issue: 2 Pages: 319-324
  • DOI: 10.1016/j.bbrc.2013.05.101
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer (2013)
  • Author(s): Nakagawa T, Endo H, Yokoyama M, Abe J, Tamai K, Tanaka N, Sato I, Takahashi S, Kondo T, Satoh K
  • Journal Title: Biochem Biophys Res Commun Volume: 436 Pages: 319-24
• [Journal Article] Enhanced expression of long non-coding RNA HOTAIR is associated with the development of gastric cancer (2013)
  • Author(s): Endo H, Shiroki T, Nakagawa T, Yokoyama M, Tamai K, Yamanami H, Fujiya T, Sato I, Yamaguchi K, Tanaka N, Iijima K, Shimosegawa T, Sugamura K, Satoh K
  • Journal Title: PLoS ONE Volume: 8
• [Journal Article] Enhanced expression of long non-coding RNA HOTAIR is associated with the development of gastric cancer. (2013)
  • Author(s): Endo H, Shiroki T, Nakagawa T, Yokoyama M, Tamai K, Yamanami H, Fujiya T, Sato I, Yamaguchi K, Tanaka N, Iijima K, Shimosegawa T, Sugamura K, Satoh K.
  • Journal Title: PlosONE Volume: 8 Issue: 10 Pages: e77070-e77070
  • DOI: 10.1371/journal.pone.0077070
  • Peer Reviewed
• [Presentation] 非小細胞肺癌におけるHOTAIRの発現と転移 (2012)
  • Author(s): 中川隆行, 田中伸幸, 菅村和夫, 佐藤郁郎, 佐藤賢一
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌