Establishment of lung cancer cell line harboring acquired resistance to receptor tyrosine kinase inhibitor

• Project/Area Number: 24791482
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Thoracic surgery
• Research Institution: Nagoya University
• Principal Investigator: FUKUI Takayuki 名古屋大学, 医学部附属病院, 病院助教 (70463198)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 細胞株 / 肺癌 / 中皮腫 / 胸腺腫 / 分子標的治療 / EGFR

Research Abstract

We started primary culture using surgical specimens or pleural effusions from the patients with malignant tumors treated in Nagoya University. We could get surgical specimens from 20 patients in two years and succeeded in the establishment of the cell lines from a mesothelioma and 3 thymic tumor cases. However, We can not able to establish a cell line harboring acquired resistance to epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) which I planned since we could not get the specimens from the patients who were resistant to EGFR-TKI. Thus, we could not able to analyze any gene alterations associated with the EGFR gene using those cell lines. However, several cell lines from mesothelioma and thymoma was successfully primary cultured and established. The cell lines from thoracic malignant tumors except the lung cancer was relatively rare and we performed immunostaining or the graft making with the mouse and confirmed the establishment of the cell lines.

Research Products

• [Journal Article] FDG PET/CT is useful for detecting infiltration to the port site in patients with malignant pleural mesothelioma. (2014)
  • Author(s): Kawaguchi K, Taniguchi T, Usami N, Fukui T, Ishiguro F, Nakamura S, Yokoi K.
  • Journal Title: Gen Thorac Cardiovasc Surg. Volume: 62 Issue: 3 Pages: 157-162
  • DOI: 10.1007/s11748-013-0345-y
  • Peer Reviewed
• [Journal Article] Significance of the serum carcinoembryonic antigen level during the follow-up of patients with completely resected non-small-cell lung cancer. (2014)
  • Author(s): Ozeki N, Fukui T, Taniguchi T, Usami N, Kawaguchi K, Ito S, Sakao Y, Mitsudomi T, Hirakawa A, Yokoi K.
  • Journal Title: Eur J Cardiothorac Surg. Volume: 45 Issue: 4 Pages: 687-692
  • DOI: 10.1093/ejcts/ezt424
  • Peer Reviewed
• [Journal Article] The association between baseline clinical-radiological characteristics and growth of pulmonary nodules with ground-glass opacity. (2014)
  • Author(s): Kobayashi Y, Sakao Y, Deshpande GA, Fukui T, Mizuno T, Kuroda H, Sakakura N, Usami N, Yatabe Y, Mitsudomi T.
  • Journal Title: Lung Cancer. Volume: 83 Issue: 1 Pages: 61-66
  • DOI: 10.1016/j.lungcan.2013.10.017
  • Peer Reviewed
• [Journal Article] Spontaneous regression of thymic epithelial tumours (2013)
  • Author(s): Fukui T, Taniguchi T, Kawaguchi K, Yokoi K
  • Journal Title: Interact Cardiovasc Thorac Surg Volume: 18 Pages: 399-401
  • Peer Reviewed
• [Journal Article] Clinicoradiologic characteristics of patients with lung adenoarcinoma harboring EML4-ALK fusion oncogene (2013)
  • Author(s): Fukui T, Yatabe Y, Kobayashi Y, Tomizawa K, Ito S, Hatooka S, Matsuo K, Mitsudomi T
  • Journal Title: Lung Cancer Volume: 77 Issue: 2 Pages: 319-325
  • DOI: 10.1016/j.lungcan.2012.03.013
  • Peer Reviewed
• [Journal Article] Spontaneous regression of thymic epithelial tumours. (2013)
  • Author(s): Takayuki Fukui, Tetsuo Taniguchi, Koji Kawaguchi, Kohei Yokoi
  • Journal Title: Interact Cardiovasc Thorac Surg. Volume: 18 Issue: 3 Pages: 399-401
  • DOI: 10.1093/icvts/ivt496
  • Peer Reviewed
• [Journal Article] Prognostic impact of tumor size eliminating the ground glass opacity component: modified clinical T descriptors of the tumor, node, metastasis classification of lung cancer. (2013)
  • Author(s): Nakamura S, Fukui T, Taniguchi T, Usami N, Kawaguchi K, Ishiguro F, Hirakawa A, Yokoi K.
  • Journal Title: J Thorac Oncol. Volume: 8 Issue: 12 Pages: 1551-1557
  • DOI: 10.1097/jto.0000000000000020
  • Peer Reviewed
• [Presentation] 胸腺腫細胞株樹立の試み (2014)
  • Author(s): 石黒太志, 谷口哲郎, 宇佐美範恭, 川口晃司, 福井高幸, 中村彰太, 尾関直樹, 加藤毅人, 山谷千尋, 横井香平
  • Organizer: 第33回日本胸腺研究会
  • Place of Presentation: 東京大学(東京都文京区)
  • Year and Date: 2014-02-08
• [Presentation] A Comparison of Clinicopathologic Features and Survival Outcomes between the Lung Cancer Patients with Adenocarcinoma and Squamous Cell Carcinoma : Stage I Disease of Squamous Cell Carcinoma is there? (2013)
  • Author(s): T. Fukui, Y. Sakao, T. Taniguchi, N. Usami, K. Kawaguchi, F. Ishiguro, S. Nakamura, K. Yokoi
  • Organizer: 15th World Conference on Lung Cancer
  • Place of Presentation: Sydney, Australia
  • Year and Date: 2013-10-28
• [Presentation] 高齢者肺切除症例における周術期経過の比較検討 (2013)
  • Author(s): 福井高幸、尾関直樹、中村彰太、水野鉄也、石黒太志、川口晃司、石川義登、谷口哲郎、横井香平
  • Organizer: 第113回日本外科学会定期学術集会
  • Place of Presentation: 福岡国際会議場（ 福岡市）
• [Presentation] 抗凝固療法が必要な心合併症を有する肺癌切除例の周術期経過 (2013)
  • Author(s): 福井高幸、谷口哲郎、川口晃司、水野鉄也、石黒太志、中村彰太、羽切周平、尾関直樹、 横井香平
  • Organizer: 第30回日本呼吸器外科学会総会
  • Place of Presentation: 名古屋国際会議場（ 名古屋市）
• [Presentation] 心合併症を有する75歳以上高齢者肺癌切除例の周術期経過 (2013)
  • Author(s): 福井高幸、谷口哲郎、宇佐美範恭、川口晃司、石黒太志、中村彰太、尾関直樹、 山谷千尋、加藤毅人、横井香平
  • Organizer: 第66回日本胸部外科学会定期学術集会
  • Place of Presentation: 仙台国際センター（ 仙台市）
• [Presentation] A Comparison of Clinicopathologic Features and Survival Outcomes between the Lung Cancer Patients with Adenocarcinoma and Squamous Cell Carcinoma: Stage I Disease of Squamous Cell Carcinoma is there? (2013)
  • Author(s): Takayuki Fukui, Yukinori Sakao, Tetsuo Taniguchi, Noriyasu Usami, Koji Kawaguchi, Futoshi Ishiguro, Shota Nakamura, Kohei Yokoi
  • Organizer: 15th World Conference on Lung Cancer
  • Place of Presentation: シドニー、オーストラリア
• [Presentation] 腺癌と扁平上皮癌の予後および臨床病理学的相違 －扁平上皮癌にI期は存在するか？－ (2013)
  • Author(s): 福井高幸、谷口哲郎、宇佐美範恭、川口晃司、石黒太志、中村彰太、尾関直樹、 山谷千尋、加藤毅人、横井香平
  • Organizer: 第54回日本肺癌学会
  • Place of Presentation: ホテルニューオータニ東京（ 東京都千代田区）