| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Research Abstract |
We started primary culture using surgical specimens or pleural effusions from the patients with malignant tumors treated in Nagoya University. We could get surgical specimens from 20 patients in two years and succeeded in the establishment of the cell lines from a mesothelioma and 3 thymic tumor cases. However, We can not able to establish a cell line harboring acquired resistance to epidermal growth factor receptor-tyrosine kinase inhibitor (EGFR-TKI) which I planned since we could not get the specimens from the patients who were resistant to EGFR-TKI. Thus, we could not able to analyze any gene alterations associated with the EGFR gene using those cell lines. However, several cell lines from mesothelioma and thymoma was successfully primary cultured and established. The cell lines from thoracic malignant tumors except the lung cancer was relatively rare and we performed immunostaining or the graft making with the mouse and confirmed the establishment of the cell lines.
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