New therapy for spinal cord injury focused on spinal anterior horn cell

• Project/Area Number: 24791498
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cerebral neurosurgery
• Research Institution: Osaka University
• Principal Investigator: ISHIHARA Masahiro 大阪大学, 医学(系)研究科(研究院), 研究員 (00533803)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 脊髄損傷 / 廃用萎縮 / 脊髄前角神経

Research Abstract

Spinal cord injury is often followed by disuse muscle atrophy. The effect of disuse muscle atrophy on motor neurons below the level of spinal cord lesions is not fully understood. Alpha-motor neurons in L4/5 at 3 weeks postinjury showed signs of degeneration associated with disuse muscle atrophy. Muscle atrophy alone did not produce a significant alpha-motor neuronal degeneration.
Our results demonstrated GDNF was responsible for alpha-motor neuronal degeneration with disuse muscle atrophy and GDNF injection in muscle could prevent alpha-motor neuronal degeneration.

Research Products

• [Journal Article] Primary olfactory mucosal cells promote axonal outgrowth in a three-dimensional assay (2014)
  • Author(s): Ishihara M, Mochizuki-Oda N, Iwatsuki K, Kishima H, Ohnishi YI, Moriwaki T, Umegaki M, Yoshimine T
  • Journal Title: J Neurosci Res
• [Journal Article] Transplantation of Olfactory Mucosa as a Scaffold for Axonal Regeneration Following Spinal Cord Contusion in Rats (2014)
  • Author(s): Iwatsuki K, Yoshimine T, Sankai Y, Umegaki M, Ohnishi YI, Ishihara M, Moriwaki T, Oda N
  • Journal Title: Neuroscience & Medicine Volume: 4(2)
• [Journal Article] Primary olfactory mucosal cells promote axonal outgrowth in a three-dimensional assay (2014)
  • Author(s): Ishihara, Masahiro; Mochizuki-Oda, Noriko; Iwatsuki, Koichi et al.
  • Journal Title: Journal of Neuroscience Research Volume: ahead of print Issue: 7 Pages: 270-4
  • DOI: 10.1002/jnr.23367
  • Peer Reviewed
• [Journal Article] Adult olfactory sphere cells are a source of oligodendrocyte and Schwann cell progenitors (2013)
  • Author(s): Ohnishi YI, Iwatsuki K, Shinzawa K, Ishihara M, Moriwaki T, Umegaki M, Kishima H, Yoshimine T
  • Journal Title: Stem Cell Research Volume: 15;11(3) Pages: 1178-1190
• [Journal Article] Involuntary muscle spasm expressed as motor evoked potential after olfactory mucosa autograft in patients with chronic spinal cord injury and complete paraplegia (2013)
  • Author(s): Iwatsuki K, Yoshimine T, Sankai Y, Tajima F, Ishihara M, et al
  • Journal Title: Journal of Biomedical Science and Engineering Volume: (6) Pages: 9-9
• [Journal Article] Adult olfactory sphere cells are a source of oligodendrocyte and Schwann cell progenitors. (2013)
  • Author(s): Ohnishi Y, Iwatsuki K et al.
  • Journal Title: Stem Cell Research Volume: 11 Issue: 3 Pages: 1178-1190
  • DOI: 10.1016/j.scr.2013.08.005
  • Peer Reviewed
• [Journal Article] Involuntary muscle spasm expressed as motor evoked potential after olfactory mucosa autograft in patients with chronic spinal cord injury and complete paraplegia (2013)
  • Author(s): Koichi Iwatsuki, Toshiki Yoshimine et al.
  • Journal Title: J. Biomedical Science and Engineering, Volume: 6 Issue: 09 Pages: 908-916
  • DOI: 10.4236/jbise.2013.69111
  • Peer Reviewed
• [Journal Article] Transplantation of olfactory mucosa as a scaffold for axonal regeneration following spinal cord contusion in rats. (2013)
  • Author(s): Koichi Iwatsuki et al.
  • Journal Title: Neuroscience & Medicine Volume: 4 Issue: 02 Pages: 112-116
  • DOI: 10.4236/nm.2013.42018
  • Peer Reviewed
• [Journal Article] Intradural extramedullary spinal ependymoma: a case report of malignant transformation occurring (2013)
  • Author(s): Takashi Moriwaki, Koichi Iwatsuki ,Masahiro Ishihara
  • Journal Title: Asian Spine Journal Volume: 2
  • Peer Reviewed
• [Journal Article] Disuse muscle atrophy exacerbates motor neuronal degeneration caudal to the site of spinal cord injury (2012)
  • Author(s): Ohnishi Y, Iwatsuki K, Shinzawa K, Nakai Y, Ishihara M, Yoshimine T
  • Journal Title: Neuroreport Volume: 15;23(3) Pages: 157-61
• [Presentation] 脊髄損傷に対する嗅粘膜移植術 (2012)
  • Author(s): 石原 正浩
  • Organizer: 第71回日本脳神経外科学会
  • Year and Date: 2012-10-17
• [Presentation] 廃用性萎縮は脊髄損傷尾側での運動神経ニューロン変性を増悪させる. (2012)
  • Author(s): 大西論一朗 岩月幸一, 新沢康英, 石原正浩, 吉峰俊樹
  • Organizer: 第27回日本脊髄外科学会
  • Place of Presentation: 浦安
  • Year and Date: 2012-06-21
• [Presentation] 廃用性筋萎縮は脊髄損傷尾側での運動ニューロン変性を増悪させる (2012)
  • Author(s): 大西諭一郎、岩月幸一、新沢康英、石原正浩、吉峰俊樹
  • Organizer: 第27回日本脊髄外科学会
  • Place of Presentation: 浦安
• [Presentation] 脊髄損傷に対する嗅粘膜移植術 (2012)
  • Author(s): 石原正浩
  • Organizer: 第71回日本脳神経外科学会
  • Place of Presentation: 大阪