New BNCT with boron-peptide against malingnat brain tumor

• Project/Area Number: 24791502
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Cerebral neurosurgery
• Research Institution: Okayama University
• Principal Investigator: MICHIUE Hiroyuki 岡山大学, 医歯(薬)学総合研究科, 助教 (20572499)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: がん / 悪性脳腫瘍 / 粒子線治療 / ホウ素中性子捕捉療法 / ホウ素薬剤研究 / 細胞内導入 / 癌 / 癌治療 / ホウ素製剤 / PET / 膠芽腫 / ドラッグデリバリーシステム / 細胞膜通過ペプチド / BSH-peptide / がん治療 / 脳腫瘍 / ホウ素化合物 / BNCT / ペプチド

Outline of Final Research Achievements

Boron neutron capture therapy (BNCT) is an encouraging treatment under clinical investigation. In malignant cells, BNCT consists of two major factors: neutron radiation and boron uptake. To increase boron uptake in cells, we established new boron compound with peptide. New BSH-peptide will be very important drug for next generation BNCT.

Research Products

• [Journal Article] Novel leucine zipper motif-based hybrid peptide delivers functional peptide cargo inside cells. (2015)
  • Author(s): Hakata, Y., S. Tsuchiya, H. Michiue, T. Ohtsuki, H. Matsui, M. Miyazawa, and M. Kitamatsu
  • Journal Title: Chem. Comm. Volume: 51 Issue: 2 Pages: 413-416
  • DOI: 10.1039/c4cc07459a
  • Peer Reviewed / Open Access
• [Journal Article] Annexin A2 regulates angiogenesis and invasion phenotypes of malignant glioma. (2015)
  • Author(s): Onishi M, Ichikawa T, Kurozumi K, Inoue S, Maruo T, Otani Y, Fujii K, Ishida J, Shimazu Y, Yoshida K, Michiue H, Antonio Chiocca E, Date I.
  • Journal Title: Brain Tumor Pathol Volume: なし
  • Peer Reviewed
• [Journal Article] Cdk5rap1-mediated 2-methylthio modification of mitochondrial tRNAs governs protein translation and contributes to myopathy in mice and humans. (2015)
  • Author(s): Wei FY, Zhou B, Suzuki T, Miyata K, Ujihara Y, Horiguchi H, Takahashi N, Xie P, Michiue H, Fujimura A, Kaitsuka T, Matsui H, Koga Y, Mohri S, Suzuki T, Oike Y, Tomizawa K.
  • Journal Title: Cell Metab Volume: 21 Issue: 3 Pages: 413-6
  • DOI: 10.1016/j.cmet.2015.01.019
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The Use of the Ubiquitin-Proteasome System in Neurosurgery and the Clinical Applications of Protein Therapy (2014)
  • Author(s): Michiue H, Date I.
  • Journal Title: Neurological Surgery 脳神経外科 Volume: 42 Issue: 10 Pages: 899-906
  • DOI: 10.11477/mf.1436200003
  • ISSN: 0301-2603, 1882-1251
  • Year and Date: 2014-10-10
  • Peer Reviewed
• [Journal Article] The acceleration of boron neutron capture therapy using multi-linked mercaptoundecahydrododecaborate (BSH) fused cell-penetrating peptide. (2014)
  • Author(s): Michiue H, Sakurai Y, Kondo N, Kitamatsu M, Bin F, Nakajima K, Hirota Y, Kawabata S, Nishiki T, Ohmori I, Tomizawa K, Miyatake S, Ono K, Matsui H.
  • Journal Title: Biomaterials. Volume: 35 Issue: 10 Pages: 3396-3405
  • DOI: 10.1016/j.biomaterials.2013.12.055
  • Peer Reviewed
• [Journal Article] Reduced neurotoxicity with combined treatment of high-dose methotrexate, cyclophosphamide, doxorubicin, vincristine and prednisolone (M-CHOP) and deferred radiotherapy for primary central nervous system lymphoma. (2014)
  • Author(s): Ichikawa T, Kurozumi K, Michiue H, Ishida J, Maeda Y, Kondo E, Kawasaki A, Date I.
  • Journal Title: Clin Neurol Neurosurg Volume: 127 Pages: 106-11
  • DOI: 10.1016/j.clineuro.2014.10.011
  • Peer Reviewed
• [Journal Article] The acceleration of boron neutron capture therapy using multi-linked mercaptoundecahydrododecaborate (BSH) fused cell-penetrating peptide. (2014)
  • Author(s): Michiue H, Sakurai Y, Kondo N, Kitamatsu M, Bin F, Nakajima K, Hirota Y, Kawabata S, Nishiki T, Ohmori I, Tomizawa K, Miyatake S, Ono K, Matsui H.
  • Journal Title: Biomaterials Volume: 35 Pages: 3396-3405
  • Peer Reviewed
• [Journal Article] A protein transduction method using oligo-arginine (3R) for the delivery of transcription factors into cell nuclei. (2012)
  • Author(s): Hitsuda T, Michiue H, Kitamatsu M, Fujimura A, Wang F, Yamamoto T, Han XJ, Tazawa H, Uneda A, Ohmori I, Nishiki T, Tomizawa K, Matsui H.
  • Journal Title: Biomaterials. Volume: 33(18) Pages: 4665-72
  • Peer Reviewed
• [Journal Article] Combining poly-arginine with the hydrophobic counter-anion 4-(1-pyrenyl)-butyric acid for protein transduction in transdermal delivery. (2012)
  • Author(s): Candan G, Michiue H, Ishikawa S, Fujimura A, Hayashi K, Uneda A, Mori A, Ohmori I, Nishiki T, Matsui H, Tomizawa K
  • Journal Title: Biomaterials Volume: 33(27) Pages: 6468-75
  • NAID: 120005232488
  • Peer Reviewed
• [Journal Article] Bimodal anti-glioma mechanisms of cilengitide demonstrated by novel invasive glioma models (2012)
  • Author(s): Onishi M, Ichikawa T, Kurozumi K, Fujii K, Yoshida K, Inoue S, Michiue H, Chiocca EA, Kaur B, Date I.
  • Journal Title: Neuropathology Volume: 19. Pages: 1440-1789
  • NAID: 120005311964
  • Peer Reviewed
• [Presentation] The development of whitening peptide with peptide percutaneous drug delivery system (2015)
  • Author(s): 道上宏之 大久保奈々子 北松瑞生 松下博昭 西木禎一 松井秀樹
  • Organizer: 第92回 日本生理学会大会
  • Place of Presentation: 神戸国際会議場・展示場 （神戸 ポートアイランド）
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] 脳血液関門通過可能なアクチン重合抑制薬による膠芽腫に対する抗浸潤効果の検討 (2014)
  • Author(s): 道上宏之 林桂一郎 山田浩司 中山大輝 黒住和彦 市川智継 松下博昭 西木禎一 竹居孝二 富澤一仁 松井秀樹
  • Organizer: 第３２回日本脳腫瘍学会学術大会
  • Place of Presentation: シェラトン・グランデ・トーキョー・ベイ・ホテル「
  • Year and Date: 2014-11-30 – 2014-12-02
• [Presentation] 臨床応用へ向けた新規BSH-peptideの開発およびその薬物動態評価 (2014)
  • Author(s): 林 祐里、道上宏之、井口佳哉、北松瑞生、竹中文章、松下博昭、西木禎一、松井秀樹
  • Organizer: 平成26年度京都大学原子炉実験所専門研究会
  • Place of Presentation: 京都大学原子炉実験所 事務棟大会議室
  • Year and Date: 2014-09-29 – 2014-09-30
• [Presentation] Short-Arginine domainを用いた新規BSH-peptideによる臨床応用へ向けた取り組み (2014)
  • Author(s): 林祐里,道上宏之, 井口佳哉,北松瑞生,松下博昭,西木禎一,富澤一仁,松井秀樹
  • Organizer: 第11回日本中性子捕捉療法学会学術大会
  • Place of Presentation: 大阪大学（吹田キャンパス）コンベンションセンター
  • Year and Date: 2014-07-05 – 2014-07-06
• [Presentation] Novel multi-linked mercaptoundecahydrododecaborate (BSH) fused cell-penetrating peptide accelerated boron neutron capture therapy (BNCT) (2014)
  • Author(s): Michiue H, Iguchi Y, Sakurai Y, Kondo N, Kitamatsu M, Kawabata S, Miyatake, Nishiki T, Tomizawa K, Ono K, Matsui H
  • Organizer: 16th International Congress on Neutron Capture Therapy
  • Place of Presentation: Pörssitalo, Fabianinkatu 14, 00100 Helsinki, Finland
  • Year and Date: 2014-06-14 – 2014-06-19
• [Presentation] The screening and functional analysis of novel anti-tumor effect GFP-peptide (2012)
  • Author(s): 道上宏之 山口晃正 北松瑞生 藤村篤史 林桂一郎 畝田篤仁 西木禎一 大守伊織 松井秀樹
  • Organizer: 第89回日本生理学会
  • Place of Presentation: 長野県松本市
• [Presentation] Multi-linked BSH fused cell-penetrating peptide (multi-BSH-peptide) accelerated Boron Neutron Capture Therapy (2012)
  • Author(s): Hiroyuki Michiue１, Akiko Mori１, Natsuko Kondo, Feng Bin3, Yoshinori Sakurai２ ,Shinji Kawabata4, Shin-ichi Miyatake4, Koji Ono２, Kazuhito Tomizawa5, Hideki Matsui１
  • Organizer: 15ｔh International Congress on Neutron Capture Therapy
  • Place of Presentation: Tsukuba city, Japan